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The map label for this gene is fadA

Identifier: 50083600

GI number: 50083600

Start: 329211

End: 330383

Strand: Reverse

Name: fadA

Synonym: ACIAD0334

Alternate gene names: 50083600

Gene position: 330383-329211 (Counterclockwise)

Preceding gene: 50083601

Following gene: 50083597

Centisome position: 9.18

GC content: 46.55

Gene sequence:

>1173_bases
ATGACTAATTTAAATCCACGTGACGTTGTGATTGTTGATGGTGTTCGTTCTGCCATGGGCAAATCTAAAAACGGAATGTT
CCGCAATGTACGCGCAGACAGCCTCTCTGCCGAGCTCGTTCGTGCACTTGTTGCCCGCAACCAGTTTGATGTTAATGAAG
TTGAAGACTTGATTTGGGGTTGTGTCAATCAGACCCTTGAACAAGGCATGAACATTGGCCGTAATATTGTTTTATTGGCC
GATTTACCTAAAACTGTTGCAGGTCAAACCGTCAACCGTTTGTGTGGTTCTTCTATGCAAGCGATTCATACCGCAGCAGC
GCAAATTGCGACCAATCAAGGTGATATCTTCATCATTGGTGGTGTAGAGCATATGGGTCATGTTGGAATGATGCACGGTA
TCGACTTGAACCCAGAAGCATCTAAACATTACGCTAAAGCATCGAATATGATGGGTTTAACTGCCGAAATGCTGGGTCGT
ATGAATGGTATTGGCCGTGAAGAGCAAGATGCTTTTGGTGTTGAATCTCACCGCCGTGCTTGGGCTGCAACTCAAGAAGG
TCGTTTCAAAAATGAAATCGTGGGTGTTGAAGGCCACGATGCCAATGGCTTTAAAATTCTCTGCGATATCGATGAAGTGA
TTCGTCCAGATGCAAACCTTGAAGCATTCAAAGCATTACGTCCTGTGTTTGATCCGAAAGGCGGTACTGTAACGGCAGCA
ACGTCTTCAGCTTTATCTGATGGTGCTTCTGCCATGCTATTAATGTCGGCTGAGCGCGCACAAGCATTAGGCTTAAAGCC
TCGTGCAGTGATTCGCTCTATGGCAGTTGCGGGTTGTGATGCAGCGATTATGGGTTATGGCCCAGTTCCAGCAACGCAGA
AAGCACTTAAACGTGCTGGTTTATCGGTTGCAGACATCCAAACAGTTGAACTGAATGAAGCATTTGCGGCTCAAGGTTTG
TCTGTCCTTAAAGGTCTGGGACTTTATGAGAAACAAGATATCGTCAACCTAAATGGCGGCGCGATTGCACTGGGTCACCC
GCTTGGCTGCTCGGGTGCACGTATCACAACGACCCTACTTAATGTGATGGAACAACAAGACACTCAAATTGGTCTTGCTA
CGATGTGTATTGGTCTCGGCCAAGGGATCGCAACCGTGATTGAACGTGTTTAA

Upstream 100 bases:

>100_bases
AACAAGTACGCACATTTGGGTAAAGCTTATGAAGCGCCGCAACTACTGCGCGACATGGCTGAAAACAACAAGAAATTTTA
CGGTTAAGGGAGCAACGTGA

Downstream 100 bases:

>100_bases
TTAATACCAAGATAAAAAATCCCCGCTCAATGCGGGGATTTTTATTTTATCGGCCTTTTAAACCGTCTTGAATTTGCTGC
TGTAGATTTATGAGGTCATT

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTNLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWGCVNQTLEQGMNIGRNIVLLA
DLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIGGVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGR
MNGIGREEQDAFGVESHRRAWAATQEGRFKNEIVGVEGHDANGFKILCDIDEVIRPDANLEAFKALRPVFDPKGGTVTAA
TSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAGLSVADIQTVELNEAFAAQGL
SVLKGLGLYEKQDIVNLNGGAIALGHPLGCSGARITTTLLNVMEQQDTQIGLATMCIGLGQGIATVIERV

Sequences:

>Translated_390_residues
MTNLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWGCVNQTLEQGMNIGRNIVLLA
DLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIGGVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGR
MNGIGREEQDAFGVESHRRAWAATQEGRFKNEIVGVEGHDANGFKILCDIDEVIRPDANLEAFKALRPVFDPKGGTVTAA
TSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAGLSVADIQTVELNEAFAAQGL
SVLKGLGLYEKQDIVNLNGGAIALGHPLGCSGARITTTLLNVMEQQDTQIGLATMCIGLGQGIATVIERV
>Mature_389_residues
TNLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWGCVNQTLEQGMNIGRNIVLLAD
LPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIGGVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGRM
NGIGREEQDAFGVESHRRAWAATQEGRFKNEIVGVEGHDANGFKILCDIDEVIRPDANLEAFKALRPVFDPKGGTVTAAT
SSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAGLSVADIQTVELNEAFAAQGLS
VLKGLGLYEKQDIVNLNGGAIALGHPLGCSGARITTTLLNVMEQQDTQIGLATMCIGLGQGIATVIERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=36.272040302267, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=38.4223918575064, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=35.75, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4504327, Length=434, Percent_Identity=32.4884792626728, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=32.9974811083123, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI194353979, Length=242, Percent_Identity=30.9917355371901, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI48994986, Length=384, Percent_Identity=64.84375, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=41.3366336633663, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=42.7860696517413, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=41, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=30.7692307692308, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=36.9346733668342, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=34.5771144278607, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=31.75, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=30.3944315545244, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=397, Percent_Identity=27.2040302267003, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=35.0877192982456, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=32.512315270936, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=38.0952380952381, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=34, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI17137578, Length=431, Percent_Identity=31.322505800464, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=31.5656565656566, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADA_ACIAD (Q6FF69)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045110.1
- ProteinModelPortal:   Q6FF69
- SMR:   Q6FF69
- STRING:   Q6FF69
- GeneID:   2881153
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0334
- NMPDR:   fig|62977.3.peg.811
- eggNOG:   COG0183
- HOGENOM:   HBG370930
- OMA:   KMEEKVN
- PhylomeDB:   Q6FF69
- ProtClustDB:   PRK08947
- BioCyc:   ASP62977:ACIAD0334-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01620
- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02445

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 41216; Mature: 41085

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 95-95 ACT_SITE 346-346 ACT_SITE 376-376

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWG
CCCCCCCCEEEECCHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CVNQTLEQGMNIGRNIVLLADLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIG
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEE
GVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGRMNGIGREEQDAFGVESHRRA
CHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHH
WAATQEGRFKNEIVGVEGHDANGFKILCDIDEVIRPDANLEAFKALRPVFDPKGGTVTAA
HHHCCCCCCHHCEEECCCCCCCCCEEEEECHHHHCCCCCHHHHHHHHHCCCCCCCEEEEE
TSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAG
CHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCC
LSVADIQTVELNEAFAAQGLSVLKGLGLYEKQDIVNLNGGAIALGHPLGCSGARITTTLL
CCCHHEEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHHH
NVMEQQDTQIGLATMCIGLGQGIATVIERV
HHHHHHCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
TNLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWG
CCCCCCCEEEECCHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CVNQTLEQGMNIGRNIVLLADLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIG
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEE
GVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGRMNGIGREEQDAFGVESHRRA
CHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHH
WAATQEGRFKNEIVGVEGHDANGFKILCDIDEVIRPDANLEAFKALRPVFDPKGGTVTAA
HHHCCCCCCHHCEEECCCCCCCCCEEEEECHHHHCCCCCHHHHHHHHHCCCCCCCEEEEE
TSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAG
CHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCC
LSVADIQTVELNEAFAAQGLSVLKGLGLYEKQDIVNLNGGAIALGHPLGCSGARITTTLL
CCCHHEEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHHHH
NVMEQQDTQIGLATMCIGLGQGIATVIERV
HHHHHHCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA