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The map label for this gene is tolC [H]

Identifier: 50083554

GI number: 50083554

Start: 278147

End: 279514

Strand: Direct

Name: tolC [H]

Synonym: ACIAD0278

Alternate gene names: 50083554

Gene position: 278147-279514 (Clockwise)

Preceding gene: 50083548

Following gene: 50083555

Centisome position: 7.73

GC content: 42.54

Gene sequence:

>1368_bases
ATGACCATCAAAAAAATTATACTTGTGACAGGTTTATGGGGATGCTCTGTTCCTGTTTTTGCACTGGATCTTGTGGGTGT
ATATCAGCTTGCCAAAAATAATGACCCTGCCTTACAGGCCGATCAATATCAATTTCAGGCCGATCAGCTCAATTTAGGCC
TGGCAACAGGGAATTTATTACCCACAGTGACTTTAGCTGGAAATGTTACCCGTGATCGTCAGACGGTTAAAAATGATCAA
TCTTTTGAATTACCTGCTTTTGGTGATGCATCCAATGCATTGGTGAGTAATACTTCTACCAAAAAACAAGTGTCTTTAAC
CGCCAGACAGCCTATCTTTCGTATGGATGCATGGCAAGGCTACAAACAGGTTAAAACATCGGTATTGTTAAGTGAAGTCA
CCTTAAAAATACAAAAACAGCAACATATTCTTGATGTCGCTGAGGCGTATTTTAATGTGCTGCGACAGCAGGCTTTAAGC
GCTGCATATCTGCAGGAAGAGCAAGCCTTGTTGCAGCAGCTTGACATGATGAATGCCAAACTCAAACAAGGATTCGTTGC
AAAAAGTGATGTGAGTGAGGCCAATGCACAATATCAAAATGCACGTGCCAATCGTATTGCTGGAGAAGTACAAATGATGC
TGGCTCAGGAACAATTGAACCAGTATATTGGCGCAAATAGCACACAAAATTTGGCTACACTCAGACCAGATTTTCAATTC
CAGTCTCCGTATCCAGCTCAGTTGCAAGATTGGTTAATGTTAGCTCAGACACATAACTTGGCGATTCAGCAAGCTACATT
GCAACGACAGTATGCCGAGGATGGACGGCGTGTGGAAAAGGCAGCACTTTATCCACAGCTCAACGCAGTTGCCACTTATG
GTTATACCGATCAAAGCCCCGAAACATTGATTTCAACCAATGGTCAGTTTGACCAGATAGGTCTTGAGTTAAGCTGGAAT
GTCTTTAATGGTGGACAAACCCGAACTTCAATTAAAAAAGCCAGTATTTTAATCGATAAGGCCCAATCACAGCTTGATAC
GGCCAAACGCAATGCTTCAGTCGACGTTAAAAAAGCATTTTTACAACTGGAAACAGATCAATCCAAGTTGCAGGCTCGAA
AAGCGGCTATGAGTTCGGCCGAGTTAGTCTCACAGTCGTCTCGTGCCAGTTACAACGAAGGCCTCAAAACTATGGTGGAT
GTTCTTTTGGCACAAAGAAATGCTTTTTCTGCAAAGCAGGATTACTTAAATGCGCAATATGATTATCTGGTCAACGTTTT
AAAACTAAAAGCCGCGGTAGGGCAGCTTTCAGAGCAAGATCTGGCTGAGCTGAATGCATGGTTGATGCCTGAAAAAATAC
AGGACTAA

Upstream 100 bases:

>100_bases
AGAAAAATCTTTTAAATGTAAATCTATAATCATGTGAATTGTAATTTGCATGGTTTTTTCTTAGTCTACATAAGCATTGT
TCATCGTTGATCATCAATTC

Downstream 100 bases:

>100_bases
ATTTTATCGCTGTCATTAAAGTTTCATACAGAATGTGCTTTAATTCACAATTATATATGTTCAGCGCATTAAGCCGGTTC
GGAGGCTGTCTTGAGTCCAA

Product: channel-tunnel spanning the outer membrane and periplasm segregation of daughter chromosomes

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTIKKIILVTGLWGCSVPVFALDLVGVYQLAKNNDPALQADQYQFQADQLNLGLATGNLLPTVTLAGNVTRDRQTVKNDQ
SFELPAFGDASNALVSNTSTKKQVSLTARQPIFRMDAWQGYKQVKTSVLLSEVTLKIQKQQHILDVAEAYFNVLRQQALS
AAYLQEEQALLQQLDMMNAKLKQGFVAKSDVSEANAQYQNARANRIAGEVQMMLAQEQLNQYIGANSTQNLATLRPDFQF
QSPYPAQLQDWLMLAQTHNLAIQQATLQRQYAEDGRRVEKAALYPQLNAVATYGYTDQSPETLISTNGQFDQIGLELSWN
VFNGGQTRTSIKKASILIDKAQSQLDTAKRNASVDVKKAFLQLETDQSKLQARKAAMSSAELVSQSSRASYNEGLKTMVD
VLLAQRNAFSAKQDYLNAQYDYLVNVLKLKAAVGQLSEQDLAELNAWLMPEKIQD

Sequences:

>Translated_455_residues
MTIKKIILVTGLWGCSVPVFALDLVGVYQLAKNNDPALQADQYQFQADQLNLGLATGNLLPTVTLAGNVTRDRQTVKNDQ
SFELPAFGDASNALVSNTSTKKQVSLTARQPIFRMDAWQGYKQVKTSVLLSEVTLKIQKQQHILDVAEAYFNVLRQQALS
AAYLQEEQALLQQLDMMNAKLKQGFVAKSDVSEANAQYQNARANRIAGEVQMMLAQEQLNQYIGANSTQNLATLRPDFQF
QSPYPAQLQDWLMLAQTHNLAIQQATLQRQYAEDGRRVEKAALYPQLNAVATYGYTDQSPETLISTNGQFDQIGLELSWN
VFNGGQTRTSIKKASILIDKAQSQLDTAKRNASVDVKKAFLQLETDQSKLQARKAAMSSAELVSQSSRASYNEGLKTMVD
VLLAQRNAFSAKQDYLNAQYDYLVNVLKLKAAVGQLSEQDLAELNAWLMPEKIQD
>Mature_454_residues
TIKKIILVTGLWGCSVPVFALDLVGVYQLAKNNDPALQADQYQFQADQLNLGLATGNLLPTVTLAGNVTRDRQTVKNDQS
FELPAFGDASNALVSNTSTKKQVSLTARQPIFRMDAWQGYKQVKTSVLLSEVTLKIQKQQHILDVAEAYFNVLRQQALSA
AYLQEEQALLQQLDMMNAKLKQGFVAKSDVSEANAQYQNARANRIAGEVQMMLAQEQLNQYIGANSTQNLATLRPDFQFQ
SPYPAQLQDWLMLAQTHNLAIQQATLQRQYAEDGRRVEKAALYPQLNAVATYGYTDQSPETLISTNGQFDQIGLELSWNV
FNGGQTRTSIKKASILIDKAQSQLDTAKRNASVDVKKAFLQLETDQSKLQARKAAMSSAELVSQSSRASYNEGLKTMVDV
LLAQRNAFSAKQDYLNAQYDYLVNVLKLKAAVGQLSEQDLAELNAWLMPEKIQD

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=438, Percent_Identity=30.5936073059361, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50589; Mature: 50458

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKKIILVTGLWGCSVPVFALDLVGVYQLAKNNDPALQADQYQFQADQLNLGLATGNLL
CCHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHEEEEEEECCCC
PTVTLAGNVTRDRQTVKNDQSFELPAFGDASNALVSNTSTKKQVSLTARQPIFRMDAWQG
EEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCHHHHHHCC
YKQVKTSVLLSEVTLKIQKQQHILDVAEAYFNVLRQQALSAAYLQEEQALLQQLDMMNAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQGFVAKSDVSEANAQYQNARANRIAGEVQMMLAQEQLNQYIGANSTQNLATLRPDFQF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
QSPYPAQLQDWLMLAQTHNLAIQQATLQRQYAEDGRRVEKAALYPQLNAVATYGYTDQSP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
ETLISTNGQFDQIGLELSWNVFNGGQTRTSIKKASILIDKAQSQLDTAKRNASVDVKKAF
CHHEECCCCEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQLETDQSKLQARKAAMSSAELVSQSSRASYNEGLKTMVDVLLAQRNAFSAKQDYLNAQY
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHH
DYLVNVLKLKAAVGQLSEQDLAELNAWLMPEKIQD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TIKKIILVTGLWGCSVPVFALDLVGVYQLAKNNDPALQADQYQFQADQLNLGLATGNLL
CHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHEEEEEEECCCC
PTVTLAGNVTRDRQTVKNDQSFELPAFGDASNALVSNTSTKKQVSLTARQPIFRMDAWQG
EEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCHHHHHHCC
YKQVKTSVLLSEVTLKIQKQQHILDVAEAYFNVLRQQALSAAYLQEEQALLQQLDMMNAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQGFVAKSDVSEANAQYQNARANRIAGEVQMMLAQEQLNQYIGANSTQNLATLRPDFQF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
QSPYPAQLQDWLMLAQTHNLAIQQATLQRQYAEDGRRVEKAALYPQLNAVATYGYTDQSP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
ETLISTNGQFDQIGLELSWNVFNGGQTRTSIKKASILIDKAQSQLDTAKRNASVDVKKAF
CHHEECCCCEEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQLETDQSKLQARKAAMSSAELVSQSSRASYNEGLKTMVDVLLAQRNAFSAKQDYLNAQY
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHH
DYLVNVLKLKAAVGQLSEQDLAELNAWLMPEKIQD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8801424 [H]