Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 50083547

GI number: 50083547

Start: 270077

End: 271444

Strand: Direct

Name: murD [H]

Synonym: ACIAD0270

Alternate gene names: 50083547

Gene position: 270077-271444 (Clockwise)

Preceding gene: 50083546

Following gene: 50083548

Centisome position: 7.51

GC content: 46.2

Gene sequence:

>1368_bases
CTGTCACAATGCGGGGCATACATGCTAATTCAACGGGGTGGACTTAAAGTCGTTGCGGGTCTTGGAATCTCAGGGGTTTC
AGCCGTTAATTTTTTACATGAACAAGGCTACCGCGTTGCTGTCACAGATTCTCGAGAGACTCCACCTGGGCATGATCAGA
TTCCTCAAGATGTGCAAACCAGTTTTGGTCAGTTAGATACTGAACTACTCCTGCAAGCCGAAGAAATTATTTTAAGCCCA
GGACTGGCACCACAATTACCCGAAATCCAGCAAGCGATTGCACAAGGCATTCCAGTCATTGGAGATATTCAAGTCTTACG
CCGTGCGACTCAGGTTCCGATTGTTGCGATTACGGGTTCAAATGCCAAAAGTACCGTAACCACTTTGTTTGGTCAGATGG
CAAAAGATGCAGGTAAACGCGTGGCAGTAGGAGGTAATTTAGGGCGTCCAGGATTAGATTTGTTAAAAGACGAGCCTGAG
TTGCTGGTACTAGAGCTGTCAAGTTTTCAACTGGAAACGACATCGCACCTGAATGCTGAGGTGGCAGTCATATTAAATAT
GAGTGAGGATCATCTAGATCGTCATGGAGACATGCTGGGCTATCATCAGGCTAAACATCGTATTTTTCAGGGTGTAAAAA
AGGTGGTTTACAACCGTGATGACAGTTTGACACGCCCTCTGGTGCCAGATAGTACACCGATGCAAAGTTTTGGACTCAAT
GCACCCGACATTAAGCAATATGGCGTTTTACGTGAAGATGATGGAACCATGTGGCTGGCACGTGGCCGTACCCGCCTGAT
TAAGAGTTCTGAGCTGTATATTCAGGGCACACATAATATTGCCAATGCACTGGCTTGTCTGGCATTGGGTGAAGCGATAG
GTTTACCTGTCGAATCTATGCTTGAAACCTTGAAGCAGTTTAAAGGGCTTGAGCATCGCTGCGAATATGTAAAAACAGTC
AATGATGTACGTTATTATAATGATTCGAAAGGGACCAATGTGGGGGCAACGCTTGCTGCGATTGATGGTCTTGGTGCTGC
AATTGAACCCAAACAAGGCAAAGTCATTGTGATTTTGGGCGGCCAGGGTAAAGGCCAGGATTTTAGCTTGCTGCGCCAGT
CTGTTGAGAAATATGTGAAGGCTGCGATTCTGATCGGTGAAGATGCTCAGCAGATTGAGCAGGCGTTGACAGAAACAACT
CAGTTGGTGCATGCATCCACTTTAAAAGATGCGGTGACACAGGCACAACAGTATGCACAGCCTGAAGACGTCGTGTTATT
GTCACCTGCATGTGCAAGTTTTGATATGTTTAAAGGTTATCCAGATCGGGGACATCAATTTGTTGCCTGTGTGGATGCGC
TGGTTTAA

Upstream 100 bases:

>100_bases
TTGACGGCTTTTTTACGCGCTGACACAAAAAACGTTGAATATAAGTGAAACTGAAAAAGCATTCAAAACCCCAAAGTGCT
AACATTTTGCCAATCAATTT

Downstream 100 bases:

>100_bases
AACAAAAATGAGAAGGAAAATGTGATGGCTGGCTTTGCTCAGACAACCATAACCAAGATTAATCAGTTTTATGAACGCTG
GATGCCACGCCTGCCCGCAG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSQCGAYMLIQRGGLKVVAGLGISGVSAVNFLHEQGYRVAVTDSRETPPGHDQIPQDVQTSFGQLDTELLLQAEEIILSP
GLAPQLPEIQQAIAQGIPVIGDIQVLRRATQVPIVAITGSNAKSTVTTLFGQMAKDAGKRVAVGGNLGRPGLDLLKDEPE
LLVLELSSFQLETTSHLNAEVAVILNMSEDHLDRHGDMLGYHQAKHRIFQGVKKVVYNRDDSLTRPLVPDSTPMQSFGLN
APDIKQYGVLREDDGTMWLARGRTRLIKSSELYIQGTHNIANALACLALGEAIGLPVESMLETLKQFKGLEHRCEYVKTV
NDVRYYNDSKGTNVGATLAAIDGLGAAIEPKQGKVIVILGGQGKGQDFSLLRQSVEKYVKAAILIGEDAQQIEQALTETT
QLVHASTLKDAVTQAQQYAQPEDVVLLSPACASFDMFKGYPDRGHQFVACVDALV

Sequences:

>Translated_455_residues
MSQCGAYMLIQRGGLKVVAGLGISGVSAVNFLHEQGYRVAVTDSRETPPGHDQIPQDVQTSFGQLDTELLLQAEEIILSP
GLAPQLPEIQQAIAQGIPVIGDIQVLRRATQVPIVAITGSNAKSTVTTLFGQMAKDAGKRVAVGGNLGRPGLDLLKDEPE
LLVLELSSFQLETTSHLNAEVAVILNMSEDHLDRHGDMLGYHQAKHRIFQGVKKVVYNRDDSLTRPLVPDSTPMQSFGLN
APDIKQYGVLREDDGTMWLARGRTRLIKSSELYIQGTHNIANALACLALGEAIGLPVESMLETLKQFKGLEHRCEYVKTV
NDVRYYNDSKGTNVGATLAAIDGLGAAIEPKQGKVIVILGGQGKGQDFSLLRQSVEKYVKAAILIGEDAQQIEQALTETT
QLVHASTLKDAVTQAQQYAQPEDVVLLSPACASFDMFKGYPDRGHQFVACVDALV
>Mature_454_residues
SQCGAYMLIQRGGLKVVAGLGISGVSAVNFLHEQGYRVAVTDSRETPPGHDQIPQDVQTSFGQLDTELLLQAEEIILSPG
LAPQLPEIQQAIAQGIPVIGDIQVLRRATQVPIVAITGSNAKSTVTTLFGQMAKDAGKRVAVGGNLGRPGLDLLKDEPEL
LVLELSSFQLETTSHLNAEVAVILNMSEDHLDRHGDMLGYHQAKHRIFQGVKKVVYNRDDSLTRPLVPDSTPMQSFGLNA
PDIKQYGVLREDDGTMWLARGRTRLIKSSELYIQGTHNIANALACLALGEAIGLPVESMLETLKQFKGLEHRCEYVKTVN
DVRYYNDSKGTNVGATLAAIDGLGAAIEPKQGKVIVILGGQGKGQDFSLLRQSVEKYVKAAILIGEDAQQIEQALTETTQ
LVHASTLKDAVTQAQQYAQPEDVVLLSPACASFDMFKGYPDRGHQFVACVDALV

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=432, Percent_Identity=45.3703703703704, Blast_Score=317, Evalue=7e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49202; Mature: 49071

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQCGAYMLIQRGGLKVVAGLGISGVSAVNFLHEQGYRVAVTDSRETPPGHDQIPQDVQT
CCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHH
SFGQLDTELLLQAEEIILSPGLAPQLPEIQQAIAQGIPVIGDIQVLRRATQVPIVAITGS
HHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCEEEEECC
NAKSTVTTLFGQMAKDAGKRVAVGGNLGRPGLDLLKDEPELLVLELSSFQLETTSHLNAE
CCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCEEEEEECCCEEEHHCCCCCE
VAVILNMSEDHLDRHGDMLGYHQAKHRIFQGVKKVVYNRDDSLTRPLVPDSTPMQSFGLN
EEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCC
APDIKQYGVLREDDGTMWLARGRTRLIKSSELYIQGTHNIANALACLALGEAIGLPVESM
CCCHHHCCCEECCCCCEEEECCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHCCCHHHH
LETLKQFKGLEHRCEYVKTVNDVRYYNDSKGTNVGATLAAIDGLGAAIEPKQGKVIVILG
HHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEC
GQGKGQDFSLLRQSVEKYVKAAILIGEDAQQIEQALTETTQLVHASTLKDAVTQAQQYAQ
CCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDVVLLSPACASFDMFKGYPDRGHQFVACVDALV
CCCEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHC
>Mature Secondary Structure 
SQCGAYMLIQRGGLKVVAGLGISGVSAVNFLHEQGYRVAVTDSRETPPGHDQIPQDVQT
CCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHH
SFGQLDTELLLQAEEIILSPGLAPQLPEIQQAIAQGIPVIGDIQVLRRATQVPIVAITGS
HHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCEEEEECC
NAKSTVTTLFGQMAKDAGKRVAVGGNLGRPGLDLLKDEPELLVLELSSFQLETTSHLNAE
CCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCCCEEEEEECCCEEEHHCCCCCE
VAVILNMSEDHLDRHGDMLGYHQAKHRIFQGVKKVVYNRDDSLTRPLVPDSTPMQSFGLN
EEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCC
APDIKQYGVLREDDGTMWLARGRTRLIKSSELYIQGTHNIANALACLALGEAIGLPVESM
CCCHHHCCCEECCCCCEEEECCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHCCCHHHH
LETLKQFKGLEHRCEYVKTVNDVRYYNDSKGTNVGATLAAIDGLGAAIEPKQGKVIVILG
HHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEC
GQGKGQDFSLLRQSVEKYVKAAILIGEDAQQIEQALTETTQLVHASTLKDAVTQAQQYAQ
CCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDVVLLSPACASFDMFKGYPDRGHQFVACVDALV
CCCEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA