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The map label for this gene is shiA [H]

Identifier: 50083503

GI number: 50083503

Start: 223204

End: 224541

Strand: Reverse

Name: shiA [H]

Synonym: ACIAD0223

Alternate gene names: 50083503

Gene position: 224541-223204 (Counterclockwise)

Preceding gene: 50083504

Following gene: 50083502

Centisome position: 6.24

GC content: 44.62

Gene sequence:

>1338_bases
ATGGCTTCAAATTTATCATCTTCTGTCCCTTCAAATGAAAAACGTGCACGCAAAGCTGCTTTTAGTAGTTTTATCGGTGC
GGTTGTTGACTGGTACGATTTTCTCCTTTACGGCATTGTCGCTGCATTGATTTTTAATCAACAGTTTTTTCCAAGCATTA
GTCCTGCGATGGGAACTTTGGCCGCCTTTGCAACGTTTGGTGTAGGCTTCCTCTTCCGCCCGCTCGGTGGTGTGGTCTTT
GGACATTTTGGCGACCGTTTAGGTCGTAAAAAAATGCTATTTCTGACCGTATTCCTGATGGGTACTGCAACAATATTGAT
CGGTCTTTTACCCAACTTTGAAGCAATTGGCTGGTGGGCACCCGTCTTACTGGTGTTGTTGCGTGCTATTCAAGGCTTCG
CTGTCGGTGGTGAATGGGGTGGTGCTGCCTTAATGGCAGTTGAGAATGCGCCTGCGCACCGAAAAGCATTTTATAGCAGC
GGTGTACAGATCGGCTATGGCGTCGGTCTTATGTTGGCGACTGGTAGCGTATCCTTGATTATTTATATCTTTGGACAAGA
CGGCTTTGCTGACTGGGCATGGCGAATCCCTTTTCTTGCCAGTATCATTCTGGTGCTATTCGCACTTTGGGTTCGTGGCG
GTCAAGATGAGTCACATGAATTTGAACAAGAAGTGATTAAAAAACAGATCGCACCTGTTAAAATTCCTTTATTTGAAGCT
GTTCGTCAAAATCCAAAAGGTTTTTTCTACATTATCGCTTTGCGTCTAGCAGAACTGCTGACCATGTATCTGGTCACCAC
TTTTGCTCTAAATTATTCCACCAAAAATCTGGGCATGTCGTCTAGCTTATTTTTACACATTGGTCTGATGGTCGGTGCAA
TTAGCTGTTTGACGATTCCTTTCTTTGCACATATTTCAGATCGTTTTGGACGCCGACGCATGTGCGTGATTGGCGGTCTG
ATTGGTGCAATCTGTGCATTTCCATTTTTCTTTGCGCTTGAAAGTGGCTCTACCTTCTGGATCGTACTGTTTGCTATTTT
ATTGGCCAATCTGTCACATGATATGGTGGTCAGTGTTCATCAGCCTATTTTTACCGAACTCTTTGGTGCATCTTATCGTT
ATAGTGGTGCGGGCGTAGGTTATCAGGTGGCCAGTATTGTAGGCGGTGGGTTTACGCCTTTTATTGCAACAGGTCTGATC
ACACTAGGTCATGGGCACTGGCAATATGTCGCAATTTATTTAACCATCGGTTGTTTACTCACCACGTGGGTTGCAGCACG
AATGAAAGCCCAAGTACAATCAGTACATCCTTCTTCTGGTTATGTTACAGGCAAATAA

Upstream 100 bases:

>100_bases
GATACCTTTACTTATTGCATTATGTCCTGTAATTGGCGCGACGACAAAATCCGAATATTGCAGGGCATCCGACCTGAAAG
CATGGGATTCTCAATTATAA

Downstream 100 bases:

>100_bases
ATGAGCGTACAGCGCATACATGTAGAAAAACGCTTTTCTGAAATTGCCATTTCAGGCAATTTGGTTCATCTTGCGGGACA
GCTCGCAAGCGATACCCAGC

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MASNLSSSVPSNEKRARKAAFSSFIGAVVDWYDFLLYGIVAALIFNQQFFPSISPAMGTLAAFATFGVGFLFRPLGGVVF
GHFGDRLGRKKMLFLTVFLMGTATILIGLLPNFEAIGWWAPVLLVLLRAIQGFAVGGEWGGAALMAVENAPAHRKAFYSS
GVQIGYGVGLMLATGSVSLIIYIFGQDGFADWAWRIPFLASIILVLFALWVRGGQDESHEFEQEVIKKQIAPVKIPLFEA
VRQNPKGFFYIIALRLAELLTMYLVTTFALNYSTKNLGMSSSLFLHIGLMVGAISCLTIPFFAHISDRFGRRRMCVIGGL
IGAICAFPFFFALESGSTFWIVLFAILLANLSHDMVVSVHQPIFTELFGASYRYSGAGVGYQVASIVGGGFTPFIATGLI
TLGHGHWQYVAIYLTIGCLLTTWVAARMKAQVQSVHPSSGYVTGK

Sequences:

>Translated_445_residues
MASNLSSSVPSNEKRARKAAFSSFIGAVVDWYDFLLYGIVAALIFNQQFFPSISPAMGTLAAFATFGVGFLFRPLGGVVF
GHFGDRLGRKKMLFLTVFLMGTATILIGLLPNFEAIGWWAPVLLVLLRAIQGFAVGGEWGGAALMAVENAPAHRKAFYSS
GVQIGYGVGLMLATGSVSLIIYIFGQDGFADWAWRIPFLASIILVLFALWVRGGQDESHEFEQEVIKKQIAPVKIPLFEA
VRQNPKGFFYIIALRLAELLTMYLVTTFALNYSTKNLGMSSSLFLHIGLMVGAISCLTIPFFAHISDRFGRRRMCVIGGL
IGAICAFPFFFALESGSTFWIVLFAILLANLSHDMVVSVHQPIFTELFGASYRYSGAGVGYQVASIVGGGFTPFIATGLI
TLGHGHWQYVAIYLTIGCLLTTWVAARMKAQVQSVHPSSGYVTGK
>Mature_444_residues
ASNLSSSVPSNEKRARKAAFSSFIGAVVDWYDFLLYGIVAALIFNQQFFPSISPAMGTLAAFATFGVGFLFRPLGGVVFG
HFGDRLGRKKMLFLTVFLMGTATILIGLLPNFEAIGWWAPVLLVLLRAIQGFAVGGEWGGAALMAVENAPAHRKAFYSSG
VQIGYGVGLMLATGSVSLIIYIFGQDGFADWAWRIPFLASIILVLFALWVRGGQDESHEFEQEVIKKQIAPVKIPLFEAV
RQNPKGFFYIIALRLAELLTMYLVTTFALNYSTKNLGMSSSLFLHIGLMVGAISCLTIPFFAHISDRFGRRRMCVIGGLI
GAICAFPFFFALESGSTFWIVLFAILLANLSHDMVVSVHQPIFTELFGASYRYSGAGVGYQVASIVGGGFTPFIATGLIT
LGHGHWQYVAIYLTIGCLLTTWVAARMKAQVQSVHPSSGYVTGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=67.6674364896074, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1790550, Length=399, Percent_Identity=32.5814536340852, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=36.5914786967419, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=31.6708229426434, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082404, Length=391, Percent_Identity=24.2966751918159, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082231, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48395; Mature: 48263

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNLSSSVPSNEKRARKAAFSSFIGAVVDWYDFLLYGIVAALIFNQQFFPSISPAMGTL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHH
AAFATFGVGFLFRPLGGVVFGHFGDRLGRKKMLFLTVFLMGTATILIGLLPNFEAIGWWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
PVLLVLLRAIQGFAVGGEWGGAALMAVENAPAHRKAFYSSGVQIGYGVGLMLATGSVSLI
HHHHHHHHHHCCEECCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCEEEEECCCEEEE
IYIFGQDGFADWAWRIPFLASIILVLFALWVRGGQDESHEFEQEVIKKQIAPVKIPLFEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHH
VRQNPKGFFYIIALRLAELLTMYLVTTFALNYSTKNLGMSSSLFLHIGLMVGAISCLTIP
HHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FFAHISDRFGRRRMCVIGGLIGAICAFPFFFALESGSTFWIVLFAILLANLSHDMVVSVH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEHH
QPIFTELFGASYRYSGAGVGYQVASIVGGGFTPFIATGLITLGHGHWQYVAIYLTIGCLL
HHHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TTWVAARMKAQVQSVHPSSGYVTGK
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ASNLSSSVPSNEKRARKAAFSSFIGAVVDWYDFLLYGIVAALIFNQQFFPSISPAMGTL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHH
AAFATFGVGFLFRPLGGVVFGHFGDRLGRKKMLFLTVFLMGTATILIGLLPNFEAIGWWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
PVLLVLLRAIQGFAVGGEWGGAALMAVENAPAHRKAFYSSGVQIGYGVGLMLATGSVSLI
HHHHHHHHHHCCEECCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCEEEEECCCEEEE
IYIFGQDGFADWAWRIPFLASIILVLFALWVRGGQDESHEFEQEVIKKQIAPVKIPLFEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHH
VRQNPKGFFYIIALRLAELLTMYLVTTFALNYSTKNLGMSSSLFLHIGLMVGAISCLTIP
HHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FFAHISDRFGRRRMCVIGGLIGAICAFPFFFALESGSTFWIVLFAILLANLSHDMVVSVH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEHH
QPIFTELFGASYRYSGAGVGYQVASIVGGGFTPFIATGLITLGHGHWQYVAIYLTIGCLL
HHHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TTWVAARMKAQVQSVHPSSGYVTGK
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]