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The map label for this gene is def [H]

Identifier: 50083493

GI number: 50083493

Start: 207295

End: 207819

Strand: Direct

Name: def [H]

Synonym: ACIAD0211

Alternate gene names: 50083493

Gene position: 207295-207819 (Clockwise)

Preceding gene: 50083489

Following gene: 50083494

Centisome position: 5.76

GC content: 41.71

Gene sequence:

>525_bases
ATGGCCTTGTTACCAATTTTAAGTTTTCCTGATGCTCGCCTACGTACCATCGCAAAACCTGTTGAAAAAGTGACAGATGA
AATTCGTCAACTTGCAGCAGACATGCTTGAAACCATGTATGAAGCACCAGGCATTGGTCTGGCTGCAACTCAGGTTGATC
GTCATATTCAGCTCATTGTCATGGACTTGTCTGAAGAGAAAGATCAGCCTATGGTCTTTATTAACCCCAAAATTACCCCA
CTCACCGAAGAAACACAGCCTTATGAAGAGGGCTGCTTATCGGTTCCTCAAATATACGACAAAGTTAACCGTACGTCACG
TGTCAAGATTGAGGCAATTAATCTGAATGATGAAGCATTTGAGATTGAAGCTGACGGTCTCCTGGCTGTATGTATTCAAC
ATGAAATGGATCACTTAAATGGCAAATTATTTGTCGATTATTTATCGCCATTAAAGCGCCAAAGAGCCCGCGAAAAAGTT
GAAAAAGTAGTACGCCAACGTGAAAAAGTAGCGGTAAAACGTTAG

Upstream 100 bases:

>100_bases
ATGTCTGTAGTTGCGTTATAATAACGACAATACACACATTTTTTTATACTGAGTTGCCTTCATTGGGTTATCTGATCAAT
GGCACAAATGAGGAGACACT

Downstream 100 bases:

>100_bases
TTTCGATTAACCTATCGCATGAACAGCCCAATTTTGATTGGGCTTTGTTATACTATAACGCTGAAACTTCTGAGAACATC
GCACTTGTTTAAAATTGGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MALLPILSFPDARLRTIAKPVEKVTDEIRQLAADMLETMYEAPGIGLAATQVDRHIQLIVMDLSEEKDQPMVFINPKITP
LTEETQPYEEGCLSVPQIYDKVNRTSRVKIEAINLNDEAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRAREKV
EKVVRQREKVAVKR

Sequences:

>Translated_174_residues
MALLPILSFPDARLRTIAKPVEKVTDEIRQLAADMLETMYEAPGIGLAATQVDRHIQLIVMDLSEEKDQPMVFINPKITP
LTEETQPYEEGCLSVPQIYDKVNRTSRVKIEAINLNDEAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRAREKV
EKVVRQREKVAVKR
>Mature_173_residues
ALLPILSFPDARLRTIAKPVEKVTDEIRQLAADMLETMYEAPGIGLAATQVDRHIQLIVMDLSEEKDQPMVFINPKITPL
TEETQPYEEGCLSVPQIYDKVNRTSRVKIEAINLNDEAFEIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRAREKVE
KVVRQREKVAVKR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=31.6129032258064, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=57.5757575757576, Blast_Score=190, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24645728, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19881; Mature: 19750

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILSFPDARLRTIAKPVEKVTDEIRQLAADMLETMYEAPGIGLAATQVDRHIQLIV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
MDLSEEKDQPMVFINPKITPLTEETQPYEEGCLSVPQIYDKVNRTSRVKIEAINLNDEAF
EECCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCEE
EIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRAREKVEKVVRQREKVAVKR
EEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLPILSFPDARLRTIAKPVEKVTDEIRQLAADMLETMYEAPGIGLAATQVDRHIQLIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
MDLSEEKDQPMVFINPKITPLTEETQPYEEGCLSVPQIYDKVNRTSRVKIEAINLNDEAF
EECCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCEE
EIEADGLLAVCIQHEMDHLNGKLFVDYLSPLKRQRAREKVEKVVRQREKVAVKR
EEECCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA