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The map label for this gene is sfcA

Identifier: 50083451

GI number: 50083451

Start: 165044

End: 166744

Strand: Direct

Name: sfcA

Synonym: ACIAD0166

Alternate gene names: 50083451

Gene position: 165044-166744 (Clockwise)

Preceding gene: 50083447

Following gene: 50083452

Centisome position: 4.59

GC content: 43.5

Gene sequence:

>1701_bases
ATGACAGCTGAAACCAATACAAATAAGCACCCATTATATATTCCTTATGCGGGATATACCCTCCTAGAACTCCCACTTCT
CAATAAGGGGTCGGCTTTTACTCAGGAAGAGCGTAGCCATTTTAATTTGCACGGTTTAATTCCGCATGTGATTGAAACCA
TTGAAGAGCAGAGCCAGCGCTCATATCAGCAATATGGCGCATTTAATGACGACATCAACAAGCATATTTATTTAAGAAAT
ATTCAAGACACCAATGAAACGCTATTTTACCGTTTGATCAATGATCATCTGGAAGAAATGATGCCGATCATCTACACACC
AACCGTTGGTGAGGCATGTCAGCGTTTCTCTGATATTTACCGCCGTCATCGCGGAATCTTTATCTCTTATCCAGATCGTG
AACATATCGATGACATTCTGCAAAATGTGAGCAAGCGCAATGTTAAAGTGATTGTAATCACAGATGGTGAGCGTATTTTG
GGTCTAGGCGATCAGGGTATCGGTGGAATGGGGATTCCAATTGGTAAACTGTCGCTTTATACCGCATGTGGTGGTATTAG
CCCTGCCTATACATTGCCAATTACCATTGACGTGGGTACCAATAATCAGCAATTACTCAACGATCCGATTTACATGGGCT
GGCGTCAGCCACGCATTAGTGGTGATGAGTATTACGAATTTATCGATCAGGTGTTAACAGGGATTCGTCGTCGTTGGCCA
CATGCTTTGATTCAGTTTGAAGATTTCGCTCAGAAAAATGCGATGCCGTTACTCACCAAGTATCGTGACAAATTCTGTTG
CTTTAATGACGATATTCAAGGAACTGCTGCAGTTTCGGTGGGCAGTTTGATTGCGGCAAGTCGTGCGGCTGGTAAACAGT
TAAAAGATCAAACCATTACCTTTTTGGGTGCAGGTTCTGCTGGATGTGGTATTGCCGAGCAGATTGTGGCACAAATGGTC
GCAGAAGGTCTGACAGATGCACAGGCGAGAGCACGGGTGTATATGGTAGATCGTTTTGGATTAATCACAGAAAATCAGCC
AAACTTGCTAGATTTCCAGCGCAAGCTTGCACAAAAAGCTGAAGTAGTATCAGACTGGGGCAACGTTGAAGAAGTCATTT
CACTACTCGATGTGGTTAAGAATGCTAAGCCAACGGTATTGATTGGTGTGTCAGGTCAACCAGGTCTATTTACCGAAGAA
GTGATTCGTACTTTAGCGGAAAATTGTGAGCGTCCGATTGTGATGCCACTGTCTAATCCAACGTCACGTGTTGAAGCATT
GCCTTCAGATGTTATTCAATGGACAAATGGTCGAGCCTTGATTGCAACAGGTAGCCCATTTGCACCAGTCAATTATCAAG
GTAAGCTCTACAATATTTCACAGTGCAACAACTCGTATATTTTTCCTGGGATTGGTTTGGGGGTGATTGCTTCAGGTGCC
AAGCGCGTGACAGACAACATGCTGATGGCTTCAAGTAATGCCTTGGCGGATTGTTCACCATTGTTGCAAAATCCAAATGC
CGACCTATTGCCAGCGATTGCTGATATTCAGGATGTTTCCAAACGTATCGCATTCAAAGTGGCGAAGGCTGCCATTGAAG
ATGGTGTGGCATTGAATATGAGTGATGAGGTGCTGTTACAGAATATTGAAAAAGAGTTCTGGAAACCCAAATATCGTGGT
TATAAACGTGTTCCGTTTTAA

Upstream 100 bases:

>100_bases
TTCGAAATGCCCATTTTGCTTCAAAACGGCTTAAGCTATAGCAGTAGATGAATAGATAAGCAACCTTTTAGGGATTGATC
CCTGAAACATGAGATAGGCA

Downstream 100 bases:

>100_bases
TCCATAATTTAAAAAAAAGAGCTTCGGCTCTTTTTTTTTTGGAAAAAAGATAACAAAGCCTATAGACGGGACTGTCTGTA
TTTTTTAAAATTTGTAGACA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQRSYQQYGAFNDDINKHIYLRN
IQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIYRRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERIL
GLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWP
HALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTITFLGAGSAGCGIAEQIVAQMV
AEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKAEVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEE
VIRTLAENCERPIVMPLSNPTSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGA
KRVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNMSDEVLLQNIEKEFWKPKYRG
YKRVPF

Sequences:

>Translated_566_residues
MTAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQRSYQQYGAFNDDINKHIYLRN
IQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIYRRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERIL
GLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWP
HALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTITFLGAGSAGCGIAEQIVAQMV
AEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKAEVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEE
VIRTLAENCERPIVMPLSNPTSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGA
KRVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNMSDEVLLQNIEKEFWKPKYRG
YKRVPF
>Mature_565_residues
TAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQRSYQQYGAFNDDINKHIYLRNI
QDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIYRRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWPH
ALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTITFLGAGSAGCGIAEQIVAQMVA
EGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKAEVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEEV
IRTLAENCERPIVMPLSNPTSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGAK
RVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNMSDEVLLQNIEKEFWKPKYRGY
KRVPF

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI4505143, Length=522, Percent_Identity=44.6360153256705, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI62420882, Length=529, Percent_Identity=42.9111531190926, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI62420880, Length=529, Percent_Identity=42.9111531190926, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI239049447, Length=529, Percent_Identity=42.9111531190926, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4505145, Length=552, Percent_Identity=42.0289855072464, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI270265879, Length=473, Percent_Identity=45.2431289640592, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI87081919, Length=561, Percent_Identity=67.7361853832442, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1788806, Length=450, Percent_Identity=24.2222222222222, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=538, Percent_Identity=43.6802973977695, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322823, Length=570, Percent_Identity=41.9298245614035, Blast_Score=449, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281362674, Length=553, Percent_Identity=45.0271247739602, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21356279, Length=553, Percent_Identity=45.0271247739602, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI281362672, Length=553, Percent_Identity=45.0271247739602, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24646388, Length=524, Percent_Identity=43.8931297709924, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24646386, Length=524, Percent_Identity=43.8931297709924, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI78707242, Length=534, Percent_Identity=38.5767790262172, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI78707238, Length=534, Percent_Identity=38.5767790262172, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI78707240, Length=515, Percent_Identity=38.6407766990291, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI78707232, Length=509, Percent_Identity=37.721021611002, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281363505, Length=534, Percent_Identity=36.3295880149813, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78707236, Length=534, Percent_Identity=36.3295880149813, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281363503, Length=532, Percent_Identity=36.0902255639098, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI19922384, Length=534, Percent_Identity=28.4644194756554, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO1_ACIAD (Q6FFL8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044961.1
- ProteinModelPortal:   Q6FFL8
- SMR:   Q6FFL8
- STRING:   Q6FFL8
- GeneID:   2879295
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0166
- NMPDR:   fig|62977.3.peg.256
- eggNOG:   COG0281
- HOGENOM:   HBG289821
- OMA:   IQDNNET
- PhylomeDB:   Q6FFL8
- ProtClustDB:   PRK13529
- BioCyc:   ASP62977:ACIAD0166-MONOMER
- HAMAP:   MF_01619
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 62970; Mature: 62839

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 105-105 ACT_SITE 176-176 BINDING 158-158 BINDING 271-271 BINDING 419-419

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQR
CCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
SYQQYGAFNDDINKHIYLRNIQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIY
HHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERILGLGDQGIGGMGIPIGKLSLY
HHHCCEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHH
TACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWP
HHHCCCCCCEEEEEEEEECCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTIT
HHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
FLGAGSAGCGIAEQIVAQMVAEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKA
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEEECCEEECCCCCHHHHHHHHHHHH
EVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEEVIRTLAENCERPIVMPLSNP
HHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
TSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGA
HHHHHHCHHHHHEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCEECCCCCHHHHHCCH
KRVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNM
HHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
SDEVLLQNIEKEFWKPKYRGYKRVPF
CHHHHHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
TAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQR
CCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
SYQQYGAFNDDINKHIYLRNIQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIY
HHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERILGLGDQGIGGMGIPIGKLSLY
HHHCCEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHH
TACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWP
HHHCCCCCCEEEEEEEEECCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTIT
HHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
FLGAGSAGCGIAEQIVAQMVAEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKA
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHEEEEEEEECCEEECCCCCHHHHHHHHHHHH
EVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEEVIRTLAENCERPIVMPLSNP
HHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
TSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGA
HHHHHHCHHHHHEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCEECCCCCHHHHHCCH
KRVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNM
HHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
SDEVLLQNIEKEFWKPKYRGYKRVPF
CHHHHHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA