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The map label for this gene is vipB [H]

Identifier: 50083379

GI number: 50083379

Start: 84248

End: 85279

Strand: Direct

Name: vipB [H]

Synonym: ACIAD0088

Alternate gene names: 50083379

Gene position: 84248-85279 (Clockwise)

Preceding gene: 50083378

Following gene: 50083380

Centisome position: 2.34

GC content: 47.09

Gene sequence:

>1032_bases
ATGACAGCTTATCAAAAACAATGTATCACATTGCAGGCCGCACCCAAGACTTGGTTGATTACAGGCGTTGCAGGGTTTAT
TGGCTCAAATTTGCTTGAGACACTGCTTAAGCTTGATCAACGCGTGATTGGGCTAGATAACTTTGCCACAGGGCACCAGC
ACAATCTGGATGAAGTTCAAGGTTTGGTGAGTCGCGAGCAGTGGAATAATTTTAAGTTTATTCGCGGTGATATCTGTCAG
TTGCAGGACTGTCAGGCTGCATGTGAAGGCGTGGATTATGTACTGCATCAGGCTGCACTGGGTTCTGTGCCACGCTCATT
AGTCGACCCGATTACGACCAATAGCGTGAACATTGGCGGCTTCTTAAATATGCTGGTGGCTGCGCGTGATGCACAAGTCA
AAAGTTTTACCTATGCTGCCAGTAGCTCCACTTATGGTGACCATCCTGCCTTGCCAAAACTGGAAGATCATATTGGCAAA
CCGCTTTCACCGTATGCGGTCACCAAATATGTTAATGAGCTGTATGCCGATGTATTTGCACGCAGTTATGGCTTTTACTG
TATTGGACTGCGTTACTTTAATGTATTTGGGCAGCGTCAGGACCCAAATGGTGCCTATGCAGCGGTCATCCCAAAATGGA
CCGCAGCCATGATTCAGGGCGAGGATGTTTTTATCAATGGTGATGGTGAAACCAGTCGCGATTTCTGTTTTATCGAAAAT
ACGGTTCAGGCCAATCTTTTGGCAGCCACAACGCAAGATGCAGCCGCTAAAAATCAGGTGTATAACGTGGCGGTGGGGGA
TCGAACCACACTTAACCAGTTATTTGCCACACTACAAAGTGCTCTGGCTGAAAATGGGATTCATTACAACAAGCCTGCGG
TATATCGTGAGTTTCGTGCAGGTGATGTGCGCCATTCACAGGCTGATATTGGCAAGATTAGTCAGTTACTGGGGTATCAA
CCAGAATACAGATTTGCGCAAGGGGTTCATAAAGCCATGCATTGGTATGTGCAGGACCTGACCTCAGCCTGA

Upstream 100 bases:

>100_bases
GACGCAGTAACCATGTGATTTATGATCTGAAATATGTGCTGGATCGTCAGCAGTCAGATATTCGTCTGTAAGTCGCACAC
TCAGGATGGATGATATGGTC

Downstream 100 bases:

>100_bases
TGTATAAAAAACTCCTGTCATCTTCGGTACTGGTGTTGCTGATTGGCATCGCCGTCAGCGCACTCGGTTTTATTAAGGAG
ATGCTGGTGGCGTACCATTT

Product: NAD-dependent epimerase/dehydratase (WbpP)

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTAYQKQCITLQAAPKTWLITGVAGFIGSNLLETLLKLDQRVIGLDNFATGHQHNLDEVQGLVSREQWNNFKFIRGDICQ
LQDCQAACEGVDYVLHQAALGSVPRSLVDPITTNSVNIGGFLNMLVAARDAQVKSFTYAASSSTYGDHPALPKLEDHIGK
PLSPYAVTKYVNELYADVFARSYGFYCIGLRYFNVFGQRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIEN
TVQANLLAATTQDAAAKNQVYNVAVGDRTTLNQLFATLQSALAENGIHYNKPAVYREFRAGDVRHSQADIGKISQLLGYQ
PEYRFAQGVHKAMHWYVQDLTSA

Sequences:

>Translated_343_residues
MTAYQKQCITLQAAPKTWLITGVAGFIGSNLLETLLKLDQRVIGLDNFATGHQHNLDEVQGLVSREQWNNFKFIRGDICQ
LQDCQAACEGVDYVLHQAALGSVPRSLVDPITTNSVNIGGFLNMLVAARDAQVKSFTYAASSSTYGDHPALPKLEDHIGK
PLSPYAVTKYVNELYADVFARSYGFYCIGLRYFNVFGQRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIEN
TVQANLLAATTQDAAAKNQVYNVAVGDRTTLNQLFATLQSALAENGIHYNKPAVYREFRAGDVRHSQADIGKISQLLGYQ
PEYRFAQGVHKAMHWYVQDLTSA
>Mature_342_residues
TAYQKQCITLQAAPKTWLITGVAGFIGSNLLETLLKLDQRVIGLDNFATGHQHNLDEVQGLVSREQWNNFKFIRGDICQL
QDCQAACEGVDYVLHQAALGSVPRSLVDPITTNSVNIGGFLNMLVAARDAQVKSFTYAASSSTYGDHPALPKLEDHIGKP
LSPYAVTKYVNELYADVFARSYGFYCIGLRYFNVFGQRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIENT
VQANLLAATTQDAAAKNQVYNVAVGDRTTLNQLFATLQSALAENGIHYNKPAVYREFRAGDVRHSQADIGKISQLLGYQP
EYRFAQGVHKAMHWYVQDLTSA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=28.2282282282282, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI7657641, Length=336, Percent_Identity=26.7857142857143, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=24.7093023255814, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=24.7093023255814, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=24.7093023255814, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI193211614, Length=285, Percent_Identity=25.2631578947368, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI8393516, Length=285, Percent_Identity=25.2631578947368, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=25.8928571428571, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=26.8571428571429, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=26.453488372093, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=25.2279635258359, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.1363636363636, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=25.9887005649718, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=25.4335260115607, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=356, Percent_Identity=23.876404494382, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=25.7396449704142, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37881; Mature: 37750

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYQKQCITLQAAPKTWLITGVAGFIGSNLLETLLKLDQRVIGLDNFATGHQHNLDEVQ
CCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GLVSREQWNNFKFIRGDICQLQDCQAACEGVDYVLHQAALGSVPRSLVDPITTNSVNIGG
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEHHH
FLNMLVAARDAQVKSFTYAASSSTYGDHPALPKLEDHIGKPLSPYAVTKYVNELYADVFA
HHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
RSYGFYCIGLRYFNVFGQRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIEN
HHCCCEEEHHHHHHHHCCCCCCCCCEEEECCHHHHHEEECCEEEEECCCCCCCCEEEEEC
TVQANLLAATTQDAAAKNQVYNVAVGDRTTLNQLFATLQSALAENGIHYNKPAVYREFRA
CHHHHHEEECCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GDVRHSQADIGKISQLLGYQPEYRFAQGVHKAMHWYVQDLTSA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAYQKQCITLQAAPKTWLITGVAGFIGSNLLETLLKLDQRVIGLDNFATGHQHNLDEVQ
CCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
GLVSREQWNNFKFIRGDICQLQDCQAACEGVDYVLHQAALGSVPRSLVDPITTNSVNIGG
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEHHH
FLNMLVAARDAQVKSFTYAASSSTYGDHPALPKLEDHIGKPLSPYAVTKYVNELYADVFA
HHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
RSYGFYCIGLRYFNVFGQRQDPNGAYAAVIPKWTAAMIQGEDVFINGDGETSRDFCFIEN
HHCCCEEEHHHHHHHHCCCCCCCCCEEEECCHHHHHEEECCEEEEECCCCCCCCEEEEEC
TVQANLLAATTQDAAAKNQVYNVAVGDRTTLNQLFATLQSALAENGIHYNKPAVYREFRA
CHHHHHEEECCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GDVRHSQADIGKISQLLGYQPEYRFAQGVHKAMHWYVQDLTSA
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]