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The map label for this gene is epsM [H]

Identifier: 50083377

GI number: 50083377

Start: 81192

End: 82610

Strand: Reverse

Name: epsM [H]

Synonym: ACIAD0086

Alternate gene names: 50083377

Gene position: 82610-81192 (Counterclockwise)

Preceding gene: 50083394

Following gene: 50083363

Centisome position: 2.3

GC content: 42.14

Gene sequence:

>1419_bases
ATGACAACTAAAATCTTACCTGTCATCATGGCAGGTGGTAGTGGTACACGATTATGGCCCTTATCACGTACACAATATCC
GAAACAATTTTTAAAACTTTCTCCTGATGGCTATACATTATTACAAGCCACGCTATTGCGCTTAAAAAATCTGGATTGTG
CAGACCCACTACTCATTTGTAATGAAGAGCATCGCTTTTTGGCAGCCGAGCAAATGCGTGAAATAGGTATCTCTGCCAAG
ATTATTCTGGAGCCAGAAGGTAAAAATACTGCACCTGCAATTACTCTTGCTGCACTGTATCAAATTCAACAGGCTCAAGA
CTCAGATACAATCATGCTAGTGTTAGCAGCCGATCATGTCATTACCGAACAAGACAAATTTGAACAGAGTATTACGCAAG
CACTAGAACTTGCCAAACTCGATAAACTGGTCACCTTCGGGATTGTGCCTACACATGCAGAGACAGGCTACGGTTATATC
GAAAAAGGCAACAGCGAGCTTAATGGCTTTCAGGTACAACGCTTCGTAGAGAAACCCGATGCTGCCACAGCCCAAGAATA
CTTAGAGAGTCAGAAGTTTCTTTGGAACAGCGGCATGTTCATGTTTAAAGCCGATATTTATTTAAACGAACTGCAACAGC
ATGCACAGGATATTTATAACTCATGCGTTGCTTCCATGCAGGACACCAAAGCAGATCTAGATTTTATCCGTATTGATAAA
GAGGCCTTTAAGCAATGCCGTTCTGAGTCAATTGACTATGCTGTGATGGAACAAACCAAAGATGCTGTGGTCGTGCCGCT
CGATGCCAATTGGAACGATGTTGGCTCTTGGTCTGCGCTTTGGGATATTCAGGATAAAGACGACCAAGGCAATGTGATTC
AAGGTGATGTCATTACTGTAAAAAGCCAAAATAATTATGTCTTTAGCGAAAGCCGTTTGGTCAGTTTACTGGGCGTTGAC
AATCTGGTGGTGATTGAAACCAAAGATGCGATACTGGTTGCAGACAAATCCAAAGTACAAGACATTAAAAAGATTGTCGA
AAGTATTAAGGAGCAAGGCCGTACTGAACATTTCTGCCACCGCGAAGTGTACAGACCATGGGGCAAATACGATAGTATTG
ACCATGCAGAGCGCTATCAGGTAAAACGAATTACGGTGAAACCTGGTCAGAAGCTCTCGATCCAGATGCATCATCATCGC
TCTGAACACTGGATTGTGGTGAATGGCACCGCCAAAATTCACAAAGGAAAAGAGTCTTTTTTACTCACAGAAAATCAATC
GACTTATATTCCTTTAGGTGAGATACATGCCCTTGAAAATCCAGGTAAAGTGCCTTTAGAGCTGATTGAAGTACAATCGG
GATCTTACTTAGGTGAAGATGATATTGTTCGTTTCGAAGACTTATATGGTCGTTGTTGA

Upstream 100 bases:

>100_bases
TACATGGCTTTATTTTTTTTTACAAAGCAATCATGATGCTCAATTTTTAGTGTATATCTAATTTCTGTATTCAGGTACAT
ATTCAAAGTAAAGGTTTAAC

Downstream 100 bases:

>100_bases
TTCGAAAACGAAATGGATAAAATAACTTTATCCATTTCGTTGTTTTAAGCATTTTCCATTGAGTGAGCTTCTCTGAGCAT
CATACAACCAGCCCCACTTA

Product: bifunctional mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTTKILPVIMAGGSGTRLWPLSRTQYPKQFLKLSPDGYTLLQATLLRLKNLDCADPLLICNEEHRFLAAEQMREIGISAK
IILEPEGKNTAPAITLAALYQIQQAQDSDTIMLVLAADHVITEQDKFEQSITQALELAKLDKLVTFGIVPTHAETGYGYI
EKGNSELNGFQVQRFVEKPDAATAQEYLESQKFLWNSGMFMFKADIYLNELQQHAQDIYNSCVASMQDTKADLDFIRIDK
EAFKQCRSESIDYAVMEQTKDAVVVPLDANWNDVGSWSALWDIQDKDDQGNVIQGDVITVKSQNNYVFSESRLVSLLGVD
NLVVIETKDAILVADKSKVQDIKKIVESIKEQGRTEHFCHREVYRPWGKYDSIDHAERYQVKRITVKPGQKLSIQMHHHR
SEHWIVVNGTAKIHKGKESFLLTENQSTYIPLGEIHALENPGKVPLELIEVQSGSYLGEDDIVRFEDLYGRC

Sequences:

>Translated_472_residues
MTTKILPVIMAGGSGTRLWPLSRTQYPKQFLKLSPDGYTLLQATLLRLKNLDCADPLLICNEEHRFLAAEQMREIGISAK
IILEPEGKNTAPAITLAALYQIQQAQDSDTIMLVLAADHVITEQDKFEQSITQALELAKLDKLVTFGIVPTHAETGYGYI
EKGNSELNGFQVQRFVEKPDAATAQEYLESQKFLWNSGMFMFKADIYLNELQQHAQDIYNSCVASMQDTKADLDFIRIDK
EAFKQCRSESIDYAVMEQTKDAVVVPLDANWNDVGSWSALWDIQDKDDQGNVIQGDVITVKSQNNYVFSESRLVSLLGVD
NLVVIETKDAILVADKSKVQDIKKIVESIKEQGRTEHFCHREVYRPWGKYDSIDHAERYQVKRITVKPGQKLSIQMHHHR
SEHWIVVNGTAKIHKGKESFLLTENQSTYIPLGEIHALENPGKVPLELIEVQSGSYLGEDDIVRFEDLYGRC
>Mature_471_residues
TTKILPVIMAGGSGTRLWPLSRTQYPKQFLKLSPDGYTLLQATLLRLKNLDCADPLLICNEEHRFLAAEQMREIGISAKI
ILEPEGKNTAPAITLAALYQIQQAQDSDTIMLVLAADHVITEQDKFEQSITQALELAKLDKLVTFGIVPTHAETGYGYIE
KGNSELNGFQVQRFVEKPDAATAQEYLESQKFLWNSGMFMFKADIYLNELQQHAQDIYNSCVASMQDTKADLDFIRIDKE
AFKQCRSESIDYAVMEQTKDAVVVPLDANWNDVGSWSALWDIQDKDDQGNVIQGDVITVKSQNNYVFSESRLVSLLGVDN
LVVIETKDAILVADKSKVQDIKKIVESIKEQGRTEHFCHREVYRPWGKYDSIDHAERYQVKRITVKPGQKLSIQMHHHRS
EHWIVVNGTAKIHKGKESFLLTENQSTYIPLGEIHALENPGKVPLELIEVQSGSYLGEDDIVRFEDLYGRC

Specific function: Involved in the biosynthesis of the capsular polysaccharide colanic acid [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=475, Percent_Identity=58.7368421052632, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 53453; Mature: 53322

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKILPVIMAGGSGTRLWPLSRTQYPKQFLKLSPDGYTLLQATLLRLKNLDCADPLLIC
CCCEEEEEEEECCCCCEEEECCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCCCCEEEE
NEEHRFLAAEQMREIGISAKIILEPEGKNTAPAITLAALYQIQQAQDSDTIMLVLAADHV
CCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCH
ITEQDKFEQSITQALELAKLDKLVTFGIVPTHAETGYGYIEKGNSELNGFQVQRFVEKPD
HCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHCCCC
AATAQEYLESQKFLWNSGMFMFKADIYLNELQQHAQDIYNSCVASMQDTKADLDFIRIDK
CHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECH
EAFKQCRSESIDYAVMEQTKDAVVVPLDANWNDVGSWSALWDIQDKDDQGNVIQGDVITV
HHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEECCEEEE
KSQNNYVFSESRLVSLLGVDNLVVIETKDAILVADKSKVQDIKKIVESIKEQGRTEHFCH
ECCCCEEECHHHEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHH
REVYRPWGKYDSIDHAERYQVKRITVKPGQKLSIQMHHHRSEHWIVVNGTAKIHKGKESF
HHHHCCCCCCCCCCCHHHEEEEEEEECCCCEEEEEEEECCCCCEEEEECCEEEECCCCCE
LLTENQSTYIPLGEIHALENPGKVPLELIEVQSGSYLGEDDIVRFEDLYGRC
EEECCCCCEECCHHEEECCCCCCCCEEEEEECCCCCCCCCCCEEHHHHCCCC
>Mature Secondary Structure 
TTKILPVIMAGGSGTRLWPLSRTQYPKQFLKLSPDGYTLLQATLLRLKNLDCADPLLIC
CCEEEEEEEECCCCCEEEECCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCCCCCEEEE
NEEHRFLAAEQMREIGISAKIILEPEGKNTAPAITLAALYQIQQAQDSDTIMLVLAADHV
CCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCH
ITEQDKFEQSITQALELAKLDKLVTFGIVPTHAETGYGYIEKGNSELNGFQVQRFVEKPD
HCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHCCCC
AATAQEYLESQKFLWNSGMFMFKADIYLNELQQHAQDIYNSCVASMQDTKADLDFIRIDK
CHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECH
EAFKQCRSESIDYAVMEQTKDAVVVPLDANWNDVGSWSALWDIQDKDDQGNVIQGDVITV
HHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEECCEEEE
KSQNNYVFSESRLVSLLGVDNLVVIETKDAILVADKSKVQDIKKIVESIKEQGRTEHFCH
ECCCCEEECHHHEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHH
REVYRPWGKYDSIDHAERYQVKRITVKPGQKLSIQMHHHRSEHWIVVNGTAKIHKGKESF
HHHHCCCCCCCCCCCHHHEEEEEEEECCCCEEEEEEEECCCCCEEEEECCEEEECCCCCE
LLTENQSTYIPLGEIHALENPGKVPLELIEVQSGSYLGEDDIVRFEDLYGRC
EEECCCCCEECCHHEEECCCCCCCCEEEEEECCCCCCCCCCCEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1712067; 11677609 [H]