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The map label for this gene is lspA

Identifier: 50083316

GI number: 50083316

Start: 26232

End: 26771

Strand: Reverse

Name: lspA

Synonym: ACIAD0021

Alternate gene names: 50083316

Gene position: 26771-26232 (Counterclockwise)

Preceding gene: 50083317

Following gene: 50083315

Centisome position: 0.74

GC content: 40.19

Gene sequence:

>540_bases
ATGCCTAAGCTTGAAGCAAAAAAAGGCTTATTCCAGTTTTATCCGCATAATCTAATTTGGCTTGGACTTTCAATCCTTGC
CATTATTATTGATCAGTGGACAAAATGGATTGCAAGTACACATTTAAATTACGCTGATCCTGTACCTGTACTGCCTTTTT
TAAACTGGACACTGCTGCATAACTACGGTGCAGCCTTCAGTTTTCTCTCCGACGCTGGTGGCTGGCAGCATTATCTATTT
ACTGGGCTGGCGGGCATTGTTTCGATCATTTTTATTTTCTGGCTCATGCGTATGCCTAAAAATGCAATGATTTTACCTGC
GGCCATTGCCCTGATTTTGGGTGGTGCGCTGGGTAATCTAATTGATCGAATGACTTTGGGATATGTCGTGGATTTTATTC
ATATCTATTATCAAAATCATCATTTTCCAGCCTTTAACATTGCCGACAGTGCCATCACAATCGGTACAATTTTATTGTTA
ATTGACACGTTTTTTCTTGAAAAACAACGTATCCAACGCGCAGAAGTGAAGCATGACTGA

Upstream 100 bases:

>100_bases
TGCTGGCATGTTCTCCCCGATGTAAATACTCATGCAGATCATCCAGGTTTATGCGGTCGTTGCATTATTAACGTCACGGG
CAGTGGCGAAGTGAGAAAAT

Downstream 100 bases:

>100_bases
GATTATCCAACCCAACGAAGAAATTCGTATTCAGGAAGGTGCACATGTCGATTTGCATTTTTCAGTCGCCATTGAAAATG
GTGTCGAAATTGACAACACC

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPKLEAKKGLFQFYPHNLIWLGLSILAIIIDQWTKWIASTHLNYADPVPVLPFLNWTLLHNYGAAFSFLSDAGGWQHYLF
TGLAGIVSIIFIFWLMRMPKNAMILPAAIALILGGALGNLIDRMTLGYVVDFIHIYYQNHHFPAFNIADSAITIGTILLL
IDTFFLEKQRIQRAEVKHD

Sequences:

>Translated_179_residues
MPKLEAKKGLFQFYPHNLIWLGLSILAIIIDQWTKWIASTHLNYADPVPVLPFLNWTLLHNYGAAFSFLSDAGGWQHYLF
TGLAGIVSIIFIFWLMRMPKNAMILPAAIALILGGALGNLIDRMTLGYVVDFIHIYYQNHHFPAFNIADSAITIGTILLL
IDTFFLEKQRIQRAEVKHD
>Mature_178_residues
PKLEAKKGLFQFYPHNLIWLGLSILAIIIDQWTKWIASTHLNYADPVPVLPFLNWTLLHNYGAAFSFLSDAGGWQHYLFT
GLAGIVSIIFIFWLMRMPKNAMILPAAIALILGGALGNLIDRMTLGYVVDFIHIYYQNHHFPAFNIADSAITIGTILLLI
DTFFLEKQRIQRAEVKHD

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=156, Percent_Identity=41.6666666666667, Blast_Score=123, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_ACIAD (Q6FG03)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044826.1
- STRING:   Q6FG03
- MEROPS:   A08.001
- GeneID:   2878606
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0021
- NMPDR:   fig|62977.3.peg.1086
- eggNOG:   COG0597
- HOGENOM:   HBG724422
- OMA:   EYYWPAF
- PhylomeDB:   Q6FG03
- ProtClustDB:   PRK00376
- BioCyc:   ASP62977:ACIAD0021-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 20370; Mature: 20239

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 122-122 ACT_SITE 149-149

Signals:

None

Transmembrane regions:

HASH(0x13a564b4)-; HASH(0x14d5ba98)-; HASH(0x14d56c68)-; HASH(0x14d5a3f0)-; HASH(0x14d577c8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLEAKKGLFQFYPHNLIWLGLSILAIIIDQWTKWIASTHLNYADPVPVLPFLNWTLLH
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NYGAAFSFLSDAGGWQHYLFTGLAGIVSIIFIFWLMRMPKNAMILPAAIALILGGALGNL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IDRMTLGYVVDFIHIYYQNHHFPAFNIADSAITIGTILLLIDTFFLEKQRIQRAEVKHD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure 
PKLEAKKGLFQFYPHNLIWLGLSILAIIIDQWTKWIASTHLNYADPVPVLPFLNWTLLH
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NYGAAFSFLSDAGGWQHYLFTGLAGIVSIIFIFWLMRMPKNAMILPAAIALILGGALGNL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IDRMTLGYVVDFIHIYYQNHHFPAFNIADSAITIGTILLLIDTFFLEKQRIQRAEVKHD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA