Click here to switch to the map view.

The map label for this gene is dnaA

Identifier: 50083298

GI number: 50083298

Start: 201

End: 1598

Strand: Direct

Name: dnaA

Synonym: ACIAD0001

Alternate gene names: 50083298

Gene position: 201-1598 (Clockwise)

Preceding gene: NA

Following gene: 50083299

Centisome position: 0.01

GC content: 43.85

Gene sequence:

>1398_bases
ATGCTTTGGACAGACTGCTTAACTCGCTTGCGACAAGAGCTATCTGATAACGTCTTTGCGATGTGGATTCGTCCTTTAGT
AGCTGAAGAGACGACAGATAGTCTACGCCTGTATGCGCCGAACCCTTACTGGACGCGTTATATTCAGGAACATCATCTTG
AGTTGATCTCTATTCTGGTTGAACAACTTTCTGAAGGGCGGATTCGCCAGGTCGAAATTTTGGTTGACTCGCGTCCTGGT
GCAATTTTATCCCCAGCAGAACAGCCAGCGACAACCACTGCTGCTCTGTCAAGTACACCAGTTGTACCTCAGCGCGTTAA
AAAAGAAGTTGTCGAACCAGCAGCTACGCAAAGTAATAAAATTTTAAATTCAAAAAAACGTTTACTTAACCCACTGTTTA
CCTTCTCATTGTTTGTCGAGGGCCGTTCTAACCAGATGGCTGCTGAGACTTGCCGAAAAGTTTTAACTCAACTTGGTGCC
TCCCAGCACAATCCTTTGTTTTTATATGGCCCGACAGGTTTAGGTAAAACTCACTTGATGCAGGCTGTGGGTAACGCATT
ACTTCAAGCCAAACCAAATGCACGTGTGATGTATATGACGGCAGAAAGTTTTGTACAGGATTTTGTGAGCTCGTTACAAA
AAGGCAAGGTTGAAGAGTTTAAGAAAAACTGTCGTTCACTTGATCTACTTTTGGTCGATGACATTCACTTGCTGGCGGGT
AAAGAAGCCAGTCTGGTCGAATTTTTCTATACCTTTAACGCCCTATTGGATGAATCTAAACAGATTATTTTAACTTCAGA
CCGTTATCCAAAAGAATTAACCGAGCTGGATCCGCGTCTGGTGTCACGTTTTTCTTGGGGATTATCTGTTGGTGTTGAGC
CACCAGATATTGAAACGCGTATCGAGATTTTGCTTAAAAAAGCAGAAAATAGCGGTGTGGATTTACCTCGTAATTGTGCA
TTGTTTATTGCCCAGCAAGTGGTGGCCAATGTACGTGAACTGGAAGGTGCACTCAATAAAGTCGTAGCAATTGCACGTTT
TAAAGGTTCACAGATCGATTTGGATGTGGTGCGTGAATCCCTTAAAGATGTATTGGCGATTCGTGCGCGAACCATTAGTG
TGGAAAATATTCAGCGTGTGGTCAGCGAATATTTTCGTATTCCGCTCAAAGAGTTGATTGGACCGAAGCGGACACGTATC
TATGCGCGTCCACGCCAGCTGGCGATGGGATTGGCACGTGAACTGACAGGCGATAGTTTCCCTGAAATTGGGATGGCTTT
TGGCGGACGCGATCACAGTACAGTGATGCACGCCTGTGAAAAAGTACAGAGCTTAAAACAAGAAGATCCAATTTTTAATG
AGGATTATAAAAACCTGTTGCGCTTATTACAGAGTTAA

Upstream 100 bases:

>100_bases
TTCATGACAGATGTGGATAACTTGGTTAGAATGGCGACCCCTTTCATCTGAGGGGGATGTTGTGGACTATTTTTGAATTT
AAAACGGACAGGGGATTCAC

Downstream 100 bases:

>100_bases
TAGGTTACTCTTAAGTATATGTGTGTACTTTTAAGTTATTGTGATCGCAAGATTTAATGTGTGTTGCGATGCCTGGCTTA
AGGTAAAAATTCACAGCTTA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MLWTDCLTRLRQELSDNVFAMWIRPLVAEETTDSLRLYAPNPYWTRYIQEHHLELISILVEQLSEGRIRQVEILVDSRPG
AILSPAEQPATTTAALSSTPVVPQRVKKEVVEPAATQSNKILNSKKRLLNPLFTFSLFVEGRSNQMAAETCRKVLTQLGA
SQHNPLFLYGPTGLGKTHLMQAVGNALLQAKPNARVMYMTAESFVQDFVSSLQKGKVEEFKKNCRSLDLLLVDDIHLLAG
KEASLVEFFYTFNALLDESKQIILTSDRYPKELTELDPRLVSRFSWGLSVGVEPPDIETRIEILLKKAENSGVDLPRNCA
LFIAQQVVANVRELEGALNKVVAIARFKGSQIDLDVVRESLKDVLAIRARTISVENIQRVVSEYFRIPLKELIGPKRTRI
YARPRQLAMGLARELTGDSFPEIGMAFGGRDHSTVMHACEKVQSLKQEDPIFNEDYKNLLRLLQS

Sequences:

>Translated_465_residues
MLWTDCLTRLRQELSDNVFAMWIRPLVAEETTDSLRLYAPNPYWTRYIQEHHLELISILVEQLSEGRIRQVEILVDSRPG
AILSPAEQPATTTAALSSTPVVPQRVKKEVVEPAATQSNKILNSKKRLLNPLFTFSLFVEGRSNQMAAETCRKVLTQLGA
SQHNPLFLYGPTGLGKTHLMQAVGNALLQAKPNARVMYMTAESFVQDFVSSLQKGKVEEFKKNCRSLDLLLVDDIHLLAG
KEASLVEFFYTFNALLDESKQIILTSDRYPKELTELDPRLVSRFSWGLSVGVEPPDIETRIEILLKKAENSGVDLPRNCA
LFIAQQVVANVRELEGALNKVVAIARFKGSQIDLDVVRESLKDVLAIRARTISVENIQRVVSEYFRIPLKELIGPKRTRI
YARPRQLAMGLARELTGDSFPEIGMAFGGRDHSTVMHACEKVQSLKQEDPIFNEDYKNLLRLLQS
>Mature_465_residues
MLWTDCLTRLRQELSDNVFAMWIRPLVAEETTDSLRLYAPNPYWTRYIQEHHLELISILVEQLSEGRIRQVEILVDSRPG
AILSPAEQPATTTAALSSTPVVPQRVKKEVVEPAATQSNKILNSKKRLLNPLFTFSLFVEGRSNQMAAETCRKVLTQLGA
SQHNPLFLYGPTGLGKTHLMQAVGNALLQAKPNARVMYMTAESFVQDFVSSLQKGKVEEFKKNCRSLDLLLVDDIHLLAG
KEASLVEFFYTFNALLDESKQIILTSDRYPKELTELDPRLVSRFSWGLSVGVEPPDIETRIEILLKKAENSGVDLPRNCA
LFIAQQVVANVRELEGALNKVVAIARFKGSQIDLDVVRESLKDVLAIRARTISVENIQRVVSEYFRIPLKELIGPKRTRI
YARPRQLAMGLARELTGDSFPEIGMAFGGRDHSTVMHACEKVQSLKQEDPIFNEDYKNLLRLLQS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=472, Percent_Identity=46.8220338983051, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI226510964, Length=247, Percent_Identity=23.8866396761134, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_ACIAD (Q6FG21)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_044808.1
- ProteinModelPortal:   Q6FG21
- STRING:   Q6FG21
- GeneID:   2881052
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0001
- NMPDR:   fig|62977.3.peg.1006
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- PhylomeDB:   Q6FG21
- ProtClustDB:   PRK00149
- BioCyc:   ASP62977:ACIAD0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52443; Mature: 52443

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWTDCLTRLRQELSDNVFAMWIRPLVAEETTDSLRLYAPNPYWTRYIQEHHLELISILV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EQLSEGRIRQVEILVDSRPGAILSPAEQPATTTAALSSTPVVPQRVKKEVVEPAATQSNK
HHHCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHH
ILNSKKRLLNPLFTFSLFVEGRSNQMAAETCRKVLTQLGASQHNPLFLYGPTGLGKTHLM
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
QAVGNALLQAKPNARVMYMTAESFVQDFVSSLQKGKVEEFKKNCRSLDLLLVDDIHLLAG
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCHHHHCC
KEASLVEFFYTFNALLDESKQIILTSDRYPKELTELDPRLVSRFSWGLSVGVEPPDIETR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCHHHH
IEILLKKAENSGVDLPRNCALFIAQQVVANVRELEGALNKVVAIARFKGSQIDLDVVRES
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKDVLAIRARTISVENIQRVVSEYFRIPLKELIGPKRTRIYARPRQLAMGLARELTGDSF
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCHHEEEECHHHHHHHHHHHHCCCCC
PEIGMAFGGRDHSTVMHACEKVQSLKQEDPIFNEDYKNLLRLLQS
CCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLWTDCLTRLRQELSDNVFAMWIRPLVAEETTDSLRLYAPNPYWTRYIQEHHLELISILV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EQLSEGRIRQVEILVDSRPGAILSPAEQPATTTAALSSTPVVPQRVKKEVVEPAATQSNK
HHHCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHH
ILNSKKRLLNPLFTFSLFVEGRSNQMAAETCRKVLTQLGASQHNPLFLYGPTGLGKTHLM
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHH
QAVGNALLQAKPNARVMYMTAESFVQDFVSSLQKGKVEEFKKNCRSLDLLLVDDIHLLAG
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCHHHHCC
KEASLVEFFYTFNALLDESKQIILTSDRYPKELTELDPRLVSRFSWGLSVGVEPPDIETR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCHHHH
IEILLKKAENSGVDLPRNCALFIAQQVVANVRELEGALNKVVAIARFKGSQIDLDVVRES
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKDVLAIRARTISVENIQRVVSEYFRIPLKELIGPKRTRIYARPRQLAMGLARELTGDSF
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCHHEEEECHHHHHHHHHHHHCCCCC
PEIGMAFGGRDHSTVMHACEKVQSLKQEDPIFNEDYKNLLRLLQS
CCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA