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The map label for this gene is tufA

Identifier: 161349968

GI number: 161349968

Start: 868301

End: 869491

Strand: Direct

Name: tufA

Synonym: ACIAD0885

Alternate gene names: 161349968

Gene position: 868301-869491 (Clockwise)

Preceding gene: 50084094

Following gene: 50084096

Centisome position: 24.13

GC content: 46.77

Gene sequence:

>1191_bases
ATGGCTAAGGCTAAGTTTGAACGTAATAAACCACACGTAAACGTGGGCACAATTGGTCACGTTGACCATGGTAAAACAAC
TCTTACTGCTGCGATCGCAACAATCTGTGCGAAAACTTACGGCGGTGAAGCGAAAGATTACTCACAAATCGACTCAGCTC
CAGAAGAAAAAGCACGTGGTATTACCATTAATACTTCACACGTAGAATACGACTCTCCAATTCGTCACTACGCGCACGTT
GACTGCCCGGGCCACGCCGACTACGTTAAAAACATGATTACTGGTGCTGCTCAGATGGACGGCGCGATCCTTGTTTGTGC
TGCGACTGACGGTCCTATGCCACAAACTCGTGAGCACATCCTTCTTTCTCGTCAGGTAGGTGTACCTTACATCGTTGTAT
TCTTGAACAAATGCGACCTTGTAGACGACGAAGAGCTTCTTGAGCTTGTAGAAATGGAAGTTCGTGAACTTCTTTCTACT
TATGACTTCCCAGGCGACGACACTCCAGTGATTCGTGGTTCTGCGCTTAAAGCGTTAGAAGGTGATGCTGGTCAATATGG
CGAGTCTTCAGTTCTTGCGCTTGTAGAAGCACTTGACACTTACATCCCAGAACCAGAGCGCGCAATCGACAAAGCATTCT
TGATGCCAATCGAAGATGTATTCTCGATTTCTGGTCGTGGTACAGTTGTTACTGGCCGTGTAGAAGCTGGTATCGTGAAA
GTGGGCGAATCAGTTGAAATCGTAGGTATCCGTGATACTCAAACTACGACAGTAACTGGCGTTGAAATGTTCCGTAAACT
TCTTGACGAAGGCCGTGCGGGCGAGAACTGTGGTGTTCTTCTTCGTGGTACTAAGCGTGAAGACGTACAACGTGGTCAAG
TACTTGCTAAACCAGGTACAATCAAGCCGCACACTAAATTCGACGCAGAAGTATACGTACTTTCTAAAGAAGAAGGTGGT
CGTCATACTCCATTCCTTAACGGTTACCGTCCACAGTTCTATTTCCGTACCACTGACGTAACTGGTGCAATCCAGTTGAA
AGAAGGCGTTGAAATGGTAATGCCAGGTGACAACGTTGAAATGTCAGTAGAATTGATCCACCCAATCGCAATGGACCCAG
GTCTACGTTTTGCGATCCGTGAAGGTGGTCGTACTGTAGGTGCTGGTGTTGTTGCGAAAGTAACTGCATAA

Upstream 100 bases:

>100_bases
GACGAGTAATCTTTCGATTACTATAAGCCCAAACTAATTTTTAGTTAAAAACCAAGCGCTCATGAGCGATCCTCATGAGC
AGTTTATAAAGGAAAAACTC

Downstream 100 bases:

>100_bases
TCAGTGCTCGAAAAAAAAGCGTCCCCTTGGGGCGCTTTTTTTATATCTACATTTAAACATTACTTATTTAAGAATAATCC
CTTTAATTTCTCCTTCAAAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEAKDYSQIDSAPEEKARGITINTSHVEYDSPIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMPQTREHILLSRQVGVPYIVVFLNKCDLVDDEELLELVEMEVRELLST
YDFPGDDTPVIRGSALKALEGDAGQYGESSVLALVEALDTYIPEPERAIDKAFLMPIEDVFSISGRGTVVTGRVEAGIVK
VGESVEIVGIRDTQTTTVTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGTIKPHTKFDAEVYVLSKEEGG
RHTPFLNGYRPQFYFRTTDVTGAIQLKEGVEMVMPGDNVEMSVELIHPIAMDPGLRFAIREGGRTVGAGVVAKVTA

Sequences:

>Translated_396_residues
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEAKDYSQIDSAPEEKARGITINTSHVEYDSPIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMPQTREHILLSRQVGVPYIVVFLNKCDLVDDEELLELVEMEVRELLST
YDFPGDDTPVIRGSALKALEGDAGQYGESSVLALVEALDTYIPEPERAIDKAFLMPIEDVFSISGRGTVVTGRVEAGIVK
VGESVEIVGIRDTQTTTVTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGTIKPHTKFDAEVYVLSKEEGG
RHTPFLNGYRPQFYFRTTDVTGAIQLKEGVEMVMPGDNVEMSVELIHPIAMDPGLRFAIREGGRTVGAGVVAKVTA
>Mature_395_residues
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEAKDYSQIDSAPEEKARGITINTSHVEYDSPIRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCAATDGPMPQTREHILLSRQVGVPYIVVFLNKCDLVDDEELLELVEMEVRELLSTY
DFPGDDTPVIRGSALKALEGDAGQYGESSVLALVEALDTYIPEPERAIDKAFLMPIEDVFSISGRGTVVTGRVEAGIVKV
GESVEIVGIRDTQTTTVTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGTIKPHTKFDAEVYVLSKEEGGR
HTPFLNGYRPQFYFRTTDVTGAIQLKEGVEMVMPGDNVEMSVELIHPIAMDPGLRFAIREGGRTVGAGVVAKVTA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.4556962025316, Blast_Score=467, Evalue=1e-132,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.0178571428571, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=27.4157303370787, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=27.4157303370787, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=28.4431137724551, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=28.4431137724551, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=28.4431137724551, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=335, Percent_Identity=28.955223880597, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI54607086, Length=317, Percent_Identity=27.7602523659306, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=27.2388059701493, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=83.8383838383838, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=84.2639593908629, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=336, Percent_Identity=30.952380952381, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789108, Length=319, Percent_Identity=30.7210031347962, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=31.5126050420168, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=32.4137931034483, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=53.0150753768844, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=46.2337662337662, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=31.2775330396476, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=31.2775330396476, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=30.2752293577982, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32566629, Length=448, Percent_Identity=26.5625, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=447, Percent_Identity=27.2930648769575, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=447, Percent_Identity=27.2930648769575, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=28.6666666666667, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=34.6938775510204, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=275, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.8481012658228, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.1414141414141, Blast_Score=522, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=29.6380090497738, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=29.6380090497738, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=30.6122448979592, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=453, Percent_Identity=22.9580573951435, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.9245283018868, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=55.7840616966581, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=55.7840616966581, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=28, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=28, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=28, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=28, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=31.1572700296736, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=440, Percent_Identity=27.7272727272727, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=28.6184210526316, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=26.3940520446097, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=26.3940520446097, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28572034, Length=163, Percent_Identity=36.1963190184049, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_ACIAD (Q6FF97)

Other databases:

- EMBL:   CR543861
- EMBL:   CR543861
- RefSeq:   YP_045082.1
- RefSeq:   YP_045605.2
- HSSP:   P0A6N1
- ProteinModelPortal:   Q6FF97
- SMR:   Q6FF97
- STRING:   Q6FF97
- GeneID:   2880795
- GeneID:   2880796
- GenomeReviews:   CR543861_GR
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0299
- KEGG:   aci:ACIAD0885
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- PhylomeDB:   Q6FF97
- BioCyc:   ASP62977:ACIAD0299-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 42955; Mature: 42824

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEAKDYSQIDSAPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCC
ITINTSHVEYDSPIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMPQTREHI
EEEECCCCCCCCCHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLSRQVGVPYIVVFLNKCDLVDDEELLELVEMEVRELLSTYDFPGDDTPVIRGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAGQYGESSVLALVEALDTYIPEPERAIDKAFLMPIEDVFSISGRGTVVTGRVEAGIVK
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEEEEECEEE
VGESVEIVGIRDTQTTTVTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGT
ECCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCC
IKPHTKFDAEVYVLSKEEGGRHTPFLNGYRPQFYFRTTDVTGAIQLKEGVEMVMPGDNVE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEECCCCCE
MSVELIHPIAMDPGLRFAIREGGRTVGAGVVAKVTA
EEEEEEEEEECCCCCEEEEECCCCEECCCEEEEECC
>Mature Secondary Structure 
AKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEAKDYSQIDSAPEEKARG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCC
ITINTSHVEYDSPIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMPQTREHI
EEEECCCCCCCCCHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLSRQVGVPYIVVFLNKCDLVDDEELLELVEMEVRELLSTYDFPGDDTPVIRGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAGQYGESSVLALVEALDTYIPEPERAIDKAFLMPIEDVFSISGRGTVVTGRVEAGIVK
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEEEEECEEE
VGESVEIVGIRDTQTTTVTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGT
ECCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCC
IKPHTKFDAEVYVLSKEEGGRHTPFLNGYRPQFYFRTTDVTGAIQLKEGVEMVMPGDNVE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEECCCCCE
MSVELIHPIAMDPGLRFAIREGGRTVGAGVVAKVTA
EEEEEEEEEECCCCCEEEEECCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA