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The map label for this gene is eutD

Identifier: 49481657

GI number: 49481657

Start: 5130478

End: 5131449

Strand: Reverse

Name: eutD

Synonym: BT9727_5068

Alternate gene names: 49481657

Gene position: 5131449-5130478 (Counterclockwise)

Preceding gene: 49480438

Following gene: 49478982

Centisome position: 97.97

GC content: 39.09

Gene sequence:

>972_bases
GTGAGCAACTTATTTACAACAGTAAAAGAAAAAGTTCAAGGAAAAGGCATTTCTATCGTACTTCCTGAAGGAACTGATGA
AAGAATTTTAGGCGCTGCAGAGCGTTTAGCAAAAGAAGAGTTAGTAAAACCAATCTTAGTTGGTAATAAAGAAGAAATTA
GCGCAAAAGCTGCTAGCATGAATTTAACATTAGCAGGCGTTGATATTTACGATCCAGCTACATACGAAGAAATGGATGCA
ATGGTAGCATCTTTCGTTGAACGCCGTAAAGGTAAAGCAACAGAAGAAGACGCTCGCAAAATCCTTAAAGAAGAAAATTA
CTTCGGTACAATGCTTGTATACATGGGCAAAGCACACGGTTTAGTAAGTGGTGCAGCTCACTCTACAGCTGATACAGTTC
GTCCAGCACTTCAAATTATTAAAACAAAACCAGGCGTTACAAAAACTTCTGGCGTATTCATCATGGTACGTGAAGAAGAG
AAATATGTATTTGCTGATTGCGCAATTAACATTGCACCAAATAGCCAAGATTTAGCTGAAATTGGTATCGAGAGTGCGAA
AACTGCTGAACTATTCGGTATTGACCCACGCGTTGCTATGTTAAGCTTCTCTACAAAAGGTTCTGCGAAATCTCCAGAAA
CAGAAAAAGTTGTAGAAGCAACTCGCATTGCAAAAGAAATGGCTCCAGAATTAACTTTAGATGGAGAATTCCAATTCGAT
GCAGCATTCGTACCATCTGTAGCTGAGAAAAAAGCTCCAGGTTCTACAATTAAAGGTGATGCTAACGTATTCGTATTCCC
AAGCCTAGAAGCTGGTAATATTGGCTACAAAATCGCTCAACGTTTAGGTAACTTCGAAGCAGTAGGACCAATCTTACAAG
GTTTAAACATGCCTGTAAACGACCTATCTCGTGGATGTAACGAAGAAGAAGTGTACAAGTTAGCTTTAATTACAGCAGCT
CAAGCACTTTAA

Upstream 100 bases:

>100_bases
TTATTTTATCTATTTTTTGGGAGCTATAAGTTTAAAATATAGAAGAAAAGCAGTATTCTTAAAGATAGAGTTTTTTTAAC
ATAAGTAGGAGGGTTTATTC

Downstream 100 bases:

>100_bases
TTCTATAATGAATTGAAATAAAGACCGTCGCTATTATAGCGACGGTCTTTATTTTTCTAAGCCAAGTGCTTTATTATTAC
GATCAATAATACGCTGTAAA

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase; Vegetative protein 43; VEG43 [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSNLFTTVKEKVQGKGISIVLPEGTDERILGAAERLAKEELVKPILVGNKEEISAKAASMNLTLAGVDIYDPATYEEMDA
MVASFVERRKGKATEEDARKILKEENYFGTMLVYMGKAHGLVSGAAHSTADTVRPALQIIKTKPGVTKTSGVFIMVREEE
KYVFADCAINIAPNSQDLAEIGIESAKTAELFGIDPRVAMLSFSTKGSAKSPETEKVVEATRIAKEMAPELTLDGEFQFD
AAFVPSVAEKKAPGSTIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMPVNDLSRGCNEEEVYKLALITAA
QAL

Sequences:

>Translated_323_residues
MSNLFTTVKEKVQGKGISIVLPEGTDERILGAAERLAKEELVKPILVGNKEEISAKAASMNLTLAGVDIYDPATYEEMDA
MVASFVERRKGKATEEDARKILKEENYFGTMLVYMGKAHGLVSGAAHSTADTVRPALQIIKTKPGVTKTSGVFIMVREEE
KYVFADCAINIAPNSQDLAEIGIESAKTAELFGIDPRVAMLSFSTKGSAKSPETEKVVEATRIAKEMAPELTLDGEFQFD
AAFVPSVAEKKAPGSTIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMPVNDLSRGCNEEEVYKLALITAA
QAL
>Mature_322_residues
SNLFTTVKEKVQGKGISIVLPEGTDERILGAAERLAKEELVKPILVGNKEEISAKAASMNLTLAGVDIYDPATYEEMDAM
VASFVERRKGKATEEDARKILKEENYFGTMLVYMGKAHGLVSGAAHSTADTVRPALQIIKTKPGVTKTSGVFIMVREEEK
YVFADCAINIAPNSQDLAEIGIESAKTAELFGIDPRVAMLSFSTKGSAKSPETEKVVEATRIAKEMAPELTLDGEFQFDA
AFVPSVAEKKAPGSTIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMPVNDLSRGCNEEEVYKLALITAAQ
AL

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=318, Percent_Identity=46.8553459119497, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1788800, Length=304, Percent_Identity=45.3947368421053, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1788806, Length=294, Percent_Identity=31.9727891156463, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 34741; Mature: 34610

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFTTVKEKVQGKGISIVLPEGTDERILGAAERLAKEELVKPILVGNKEEISAKAASM
CCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEE
NLTLAGVDIYDPATYEEMDAMVASFVERRKGKATEEDARKILKEENYFGTMLVYMGKAHG
EEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCC
LVSGAAHSTADTVRPALQIIKTKPGVTKTSGVFIMVREEEKYVFADCAINIAPNSQDLAE
CCCCCCCCHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCEEEEEEEEEECCCCHHHHH
IGIESAKTAELFGIDPRVAMLSFSTKGSAKSPETEKVVEATRIAKEMAPELTLDGEFQFD
HCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEC
AAFVPSVAEKKAPGSTIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMPVN
HHHCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHH
DLSRGCNEEEVYKLALITAAQAL
HHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLFTTVKEKVQGKGISIVLPEGTDERILGAAERLAKEELVKPILVGNKEEISAKAASM
CHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEE
NLTLAGVDIYDPATYEEMDAMVASFVERRKGKATEEDARKILKEENYFGTMLVYMGKAHG
EEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCC
LVSGAAHSTADTVRPALQIIKTKPGVTKTSGVFIMVREEEKYVFADCAINIAPNSQDLAE
CCCCCCCCHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCEEEEEEEEEECCCCHHHHH
IGIESAKTAELFGIDPRVAMLSFSTKGSAKSPETEKVVEATRIAKEMAPELTLDGEFQFD
HCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEC
AAFVPSVAEKKAPGSTIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMPVN
HHHCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHH
DLSRGCNEEEVYKLALITAAQAL
HHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9298659 [H]