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The map label for this gene is cwlO [H]

Identifier: 49481650

GI number: 49481650

Start: 4919294

End: 4920616

Strand: Reverse

Name: cwlO [H]

Synonym: BT9727_4873

Alternate gene names: 49481650

Gene position: 4920616-4919294 (Counterclockwise)

Preceding gene: 49480388

Following gene: 49481146

Centisome position: 93.95

GC content: 37.19

Gene sequence:

>1323_bases
ATGAAAAAGCTAAAAATGGCATCTTGCGCATTAGTTGCAGGGTTAATGTTTTCAGGGCTAACACCAAATGCATTTGCAGA
AGATAAAATTTCTGACGTGAAATCACAAATTAATACACAAAATGACACTTTACATAAACAACAACAAGAGCGTGATGAAT
TACAAAAACAAATGAATGACTTAAACAAAACAATTCAAGGTTTAGATAAGTCTGTTCAAGAGAATGCTGCAAAACTTGAT
GAAACAACGAAAAAAGTCGCGGATACTGAGCAATTAATTGAAAAGAAAAATAAAGATATTGCAGAATTACAAACGAAAAT
TGCAAAACGTGAAGAGTTATTAAGAAAACGTTTAGTTGCACTTCAAGAGCAACCAAACACGAACGTTGTAACAGAAGTTC
TTGTAAACTCTAAAAACGTTGCAGATTTAGTTGATCGTTTAGCTTCTGTTTCTAAAATTCTTGAGTCTGACGAAGATATC
ATGAAAACACAACAAGAAGATCAAGCGAACGTGAAAAAAGATGTTGAAACAGTAAAAACGAAGCAAAAAGAATTAAAAGA
AGCACAAGCTCAAATTGAAACTGCTAAGAAGGAACTTGACGCTGAAAAAGCGAAAAAAGCAACAGCAGTAAACGATTTAA
GCGGTAAAATGGATACAGTTGTAAATTCAATGACAAGTACAGAAAGCCAATTAAAAGAGCTTGAACAACAGGCATTAAGA
TTACAACAAATTGCTGAAAAAGAAGCACAAGAAAAAGCTGCGCAAGAAGTTGCTGCTCAAAAACAAGCAGAACAAGCTGC
TCAGCAAGCTCAGGCTCAACAAGCTCCAGCTCAACCAGCGCAAACACAAGCAGAGCCAGCAGCTCCAGCAAACAATGACG
GACAAGATAAAAAAGAAGAGCCTAAGAAAAAAGAAGAGAAAAAAGCAACACCAGCCCCAGCAGTACCAGCTGACAATGGT
GGCGGAAGACAAGATGTTGTTGAAAAAGCTAGAGGATTAGTAGGTCTAAAATACGTATGGGGTAGTGCATCAATTTCAAA
TGGTGGATTTGACTGCAGTGGTTTAATCTCTTACGTTTATGGTTTAGGTCGTCAAGATACTAGAGGTCTATGGAACTCTG
TACAAAAGATTAGCCCATCTGAAGCAAAACCGGGAGATCTTATCTTCTTACAAGGTACTTATAGAGCAGGCGTATCTCAC
GTTGGTATTTACATTGGTGGCGGTCAAATGATTCATGCTGCTGATGAATCAACAGGTGTTACTTACGGTAGCGTAAATAG
CTCTTACAACCAAAAGCATTTCTTAGGTTACGGAAGATTATAG

Upstream 100 bases:

>100_bases
TACAAAAACGTAACATTAGCAAAAAAAATCCCGAATTATGGTATAAGTTTTATAGGTTATATACGGAAAAAAGAAAAAAG
AAAGCGGTGTAAAAAATAAA

Downstream 100 bases:

>100_bases
GATTATAATTCAATAAAAAAACGTGTATAAAGTCGATAGCTTACTATTTTATAAGTAGGTTATCGGCTTTTTTGAGTTTT
TACGAGATTATAAGAAAGGT

Product: cell wall endopeptidase

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2 [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKKLKMASCALVAGLMFSGLTPNAFAEDKISDVKSQINTQNDTLHKQQQERDELQKQMNDLNKTIQGLDKSVQENAAKLD
ETTKKVADTEQLIEKKNKDIAELQTKIAKREELLRKRLVALQEQPNTNVVTEVLVNSKNVADLVDRLASVSKILESDEDI
MKTQQEDQANVKKDVETVKTKQKELKEAQAQIETAKKELDAEKAKKATAVNDLSGKMDTVVNSMTSTESQLKELEQQALR
LQQIAEKEAQEKAAQEVAAQKQAEQAAQQAQAQQAPAQPAQTQAEPAAPANNDGQDKKEEPKKKEEKKATPAPAVPADNG
GGRQDVVEKARGLVGLKYVWGSASISNGGFDCSGLISYVYGLGRQDTRGLWNSVQKISPSEAKPGDLIFLQGTYRAGVSH
VGIYIGGGQMIHAADESTGVTYGSVNSSYNQKHFLGYGRL

Sequences:

>Translated_440_residues
MKKLKMASCALVAGLMFSGLTPNAFAEDKISDVKSQINTQNDTLHKQQQERDELQKQMNDLNKTIQGLDKSVQENAAKLD
ETTKKVADTEQLIEKKNKDIAELQTKIAKREELLRKRLVALQEQPNTNVVTEVLVNSKNVADLVDRLASVSKILESDEDI
MKTQQEDQANVKKDVETVKTKQKELKEAQAQIETAKKELDAEKAKKATAVNDLSGKMDTVVNSMTSTESQLKELEQQALR
LQQIAEKEAQEKAAQEVAAQKQAEQAAQQAQAQQAPAQPAQTQAEPAAPANNDGQDKKEEPKKKEEKKATPAPAVPADNG
GGRQDVVEKARGLVGLKYVWGSASISNGGFDCSGLISYVYGLGRQDTRGLWNSVQKISPSEAKPGDLIFLQGTYRAGVSH
VGIYIGGGQMIHAADESTGVTYGSVNSSYNQKHFLGYGRL
>Mature_440_residues
MKKLKMASCALVAGLMFSGLTPNAFAEDKISDVKSQINTQNDTLHKQQQERDELQKQMNDLNKTIQGLDKSVQENAAKLD
ETTKKVADTEQLIEKKNKDIAELQTKIAKREELLRKRLVALQEQPNTNVVTEVLVNSKNVADLVDRLASVSKILESDEDI
MKTQQEDQANVKKDVETVKTKQKELKEAQAQIETAKKELDAEKAKKATAVNDLSGKMDTVVNSMTSTESQLKELEQQALR
LQQIAEKEAQEKAAQEVAAQKQAEQAAQQAQAQQAPAQPAQTQAEPAAPANNDGQDKKEEPKKKEEKKATPAPAVPADNG
GGRQDVVEKARGLVGLKYVWGSASISNGGFDCSGLISYVYGLGRQDTRGLWNSVQKISPSEAKPGDLIFLQGTYRAGVSH
VGIYIGGGQMIHAADESTGVTYGSVNSSYNQKHFLGYGRL

Specific function: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=114, Percent_Identity=32.4561403508772, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 48060; Mature: 48060

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCCCCCHHCCCCCCC
>Mature Secondary Structure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EECCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3145906 [H]