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The map label for this gene is spo0A

Identifier: 49481644

GI number: 49481644

Start: 3995542

End: 3996372

Strand: Reverse

Name: spo0A

Synonym: BT9727_3914

Alternate gene names: 49481644

Gene position: 3996372-3995542 (Counterclockwise)

Preceding gene: 49480921

Following gene: 49478568

Centisome position: 76.3

GC content: 38.27

Gene sequence:

>831_bases
ATGTCGGAGAATCGATTCGGTAAGGGAGGAAAAGCTGTGGAGAAAATTAAAGTATGTCTTGTGGATGATAATAAAGAATT
AGTATCAATGCTAGAGAGTTATGTAGCTGCTCAAGATGATATGGAAGTAATCGGTACTGCTTATAATGGTCAAGAGTGTT
TAAATTTATTAAAAGATAAGCAGCCGGATGTACTCGTATTGGATATTATTATGCCACATTTAGATGGTTTAGCTGTACTA
GAGAAAATGCGACATATTGAAAGGTTAAGACAGCCTAGCGTAATTATGTTGACAGCATTTGGACAAGAAGATGTGACGAA
AAAAGCAGTTGACTTAGGTGCTTCATATTTCATATTAAAACCATTTGATATGGAGAATTTAACGAGTCATATTCGTCAAG
TGAGCGGTAAGGCGAACGCTACTATTAAACGTCCACTGCCATCTTTCCGATCAGCAACAACAGTAGATGGAAAACCGAAA
AACTTAGACGCGAGCATTACGAGTATCATTCATGAAATTGGTGTGCCTGCTCATATTAAAGGGTACATGTACTTAAGGGA
AGCAATTTCTATGGTGTACAATGACATCGAATTACTTGGATCTATTACGAAAGTATTGTATCCAGACATCGCGAAGAAAT
ATAATACAACAGCAAGCCGCGTTGAGCGTGCAATCCGTCACGCAATTGAAGTAGCATGGAGTCGTGGGAATATTGATTCT
ATTTCGTCCTTATTCGGTTATACAGTATCCATGTCAAAAGCAAAACCTACGAACTCCGAGTTTATTGCAATGGTTGCGGA
TAAGCTTAGACTTGAACATAAGGCTAGCTGA

Upstream 100 bases:

>100_bases
GATAAACATATTTTTTTGTGATTTTATCGGAAAGTAACAAACTTTAAAAGGGAATTTTCACACAATTGTCGAACAATTCA
TGTAGCCTAGAAGGATACAC

Downstream 100 bases:

>100_bases
GAATAGAAGAAGTAAGGGATTTAAGTTTTAATAAAGCTTAATCATTCTTGAAATTTATACTTGATAGTACATTTAAAACT
AAAGAACTTTTTATGTAATG

Product: stage 0 sporulation protein A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSENRFGKGGKAVEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVL
EKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPK
NLDASITSIIHEIGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDS
ISSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_276_residues
MSENRFGKGGKAVEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVL
EKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPK
NLDASITSIIHEIGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDS
ISSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS
>Mature_275_residues
SENRFGKGGKAVEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVLE
KMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKN
LDASITSIIHEIGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSI
SSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=112, Percent_Identity=40.1785714285714, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=36.6071428571429, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082052, Length=130, Percent_Identity=31.5384615384615, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 30672; Mature: 30541

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENRFGKGGKAVEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDK
CCCCCCCCCCHHHHHEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCC
QPDVLVLDIIMPHLDGLAVLEKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILK
CCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEC
PFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHEIGVPAHIK
CCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
GYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
ISSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SENRFGKGGKAVEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDK
CCCCCCCCCHHHHHEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCC
QPDVLVLDIIMPHLDGLAVLEKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILK
CCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEC
PFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHEIGVPAHIK
CCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
GYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
ISSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629; 7885226 [H]