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The map label for this gene is safA

Identifier: 49481532

GI number: 49481532

Start: 4222717

End: 4224546

Strand: Reverse

Name: safA

Synonym: BT9727_4161

Alternate gene names: 49481532

Gene position: 4224546-4222717 (Counterclockwise)

Preceding gene: 49478632

Following gene: 49480670

Centisome position: 80.66

GC content: 38.31

Gene sequence:

>1830_bases
TTGAAAATTCATATCGTGCAAAAAGGGGATACCCTTTGGAAAATTGCGAAAAAGTACGGAGTGGATTTTGATACGCTGAA
ACAAACAAATACACAACTTAGTAATCCGGATCTCATCATGCCAGGTATGAAAATTAAAGTACCATCAAACGGCGTTCAGG
TGAAACAACATGCTGGTGCAGGCTCAGCACCTCCAAAACAATATGTAAAAGAAGTGCAACAAAAAGAATTTGCAGCAACT
CCAACTCCACTTGGAATAGAAGATGAAGAAGAAGTTACGTATCAATCAGCGCCAATTACACAGCAGCCTGCTATGCAACA
AACACAAAAAGAAGTGCAAGTAAAACCGCAGAAGGAAATGCAAGTGAAACCACAAAAAGAAGTACAGGTAAAACCGCAGA
AGGAAATGCAGGTGAAACCGCAGAAGGAAATGCAGGTGAAACCGCAGAAGGAAATGCAGGTGAAACCGCAAAAAGAAGTG
CAGGTAAAACCACAAAAAGAAGTGCAAAAAGAAAAACCAATTCAAATAGAAAAACCGTCTGTTATCCAAAAACCACCTGT
TATAGAAAAGCAAAAACCAGCAGAGAAAGAAAACACGAAGTTTTCGGTAAATGTGTTACCACAGCCACCGCAACCACCAA
TAAAAGCGAAAAAAGAATATAAAATTTCAGATGTAATTAAAAAAGGAAGCGAATTAATTGCTCCTCAAATTACTAAAATG
AAACCTAATAACATCATTTCTCCGCAAACGAAAAAAGATAATATAGTATCACCGCAAGTAAAGAAAGAAAATGTAGGAAA
TATAGTATCACCGCAAGTAAAGAAAGAAAATGTAGGGAATATAGTATCACCGCAAGTAAAGAAAGAAAATGTAGGAAATA
TAGTATCACCGCAAGTAAAGAAAGAAAATGTAGGAAACATAGTGTCACCGCAAGTAAAGAAAGAAAATGTAGGGAATATA
GTATCACCGCAAGTGAAGAAAGAAAATGTAGGAAATATAGTGTCGCCAAATGTATCGAAAGAAAATGTAGTGATTCCGCA
AGTAATACCGCCAAATATTCAAGTGCCAAACATGATGCCGATTATGGATAACAATCAACCACCAAACATTATGCCGATTA
TGGATAACAATCAACCACCAAACATTATGCCGATCATGGATAACAATCAACCACCAAACATGATGCCAATTATGGATAAT
AATCAAATGCCAAATATGATGCCGATCATGGATAACAATCAACCACCAAACATGATGCCAATCATGGACAATAATCAACC
ACCAAACATTATGCCAATCATGGACAATAATCAACCACCAAACATCATGCCAATCATGGATAATAATCAAATGCCAAATA
TGATGCCGATTATGGATAATAATCAAATGCCAAATATGATGCCGATTATGGATAACAACCAAATGCCGAATATGATGCCG
ATTATGGATAACAATCAACCACCGAATATGATGCCATATCAAATGCCGTATCAACAGCCTATGATGCCGCCGAATCCGTA
TTATCAACAAATGCCATATCAGCAAGGAGCACCGTTTGGACCGCAATATACGTCTATGCCAAATCCAAATATGATGCCGA
TGGATAATAATATGCCACCGCTCGTGCAAGGAGAGGAAGATTGTGGATGCGGAGGAGAGAGTAGGCTATATAGTCCGCAA
CCTGGAGGGCCACAATACGCGAATCCTTTATATTATCAACCAACTCAATCAGCATATGCACCACAGCCAGGAACGATGTA
CTATCAACCTGATCCGCCAAATGTATTTGGGGAGCCAGTTTCAGAAGAAGAGGATGAAGAAGAAGTTTAA

Upstream 100 bases:

>100_bases
TCGAAGAAGTGATGAAAGTGAAAAGAAGTTAACGTTATGTAGAAAGAGTTACTTCATGAGATTTGTTACTTATAGATAAG
TTATACAGGAGGGGGAAAAT

Downstream 100 bases:

>100_bases
AAAGGTGGGATGATTCCCACCTTTTTTGTATGTAGATTCGTTCTGGAAACGGAAGATTTACACCAAATGTTAGAAAATCC
CCAGTGAAATATTAACAGTG

Product: spoVID-dependent spore coat assembly factor SafA; FtsK/SpoIIIE family protein; surface protein PspC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MKIHIVQKGDTLWKIAKKYGVDFDTLKQTNTQLSNPDLIMPGMKIKVPSNGVQVKQHAGAGSAPPKQYVKEVQQKEFAAT
PTPLGIEDEEEVTYQSAPITQQPAMQQTQKEVQVKPQKEMQVKPQKEVQVKPQKEMQVKPQKEMQVKPQKEMQVKPQKEV
QVKPQKEVQKEKPIQIEKPSVIQKPPVIEKQKPAEKENTKFSVNVLPQPPQPPIKAKKEYKISDVIKKGSELIAPQITKM
KPNNIISPQTKKDNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNI
VSPQVKKENVGNIVSPNVSKENVVIPQVIPPNIQVPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQPPNMMPIMDN
NQMPNMMPIMDNNQPPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQMPNMMPIMDNNQMPNMMPIMDNNQMPNMMP
IMDNNQPPNMMPYQMPYQQPMMPPNPYYQQMPYQQGAPFGPQYTSMPNPNMMPMDNNMPPLVQGEEDCGCGGESRLYSPQ
PGGPQYANPLYYQPTQSAYAPQPGTMYYQPDPPNVFGEPVSEEEDEEEV

Sequences:

>Translated_609_residues
MKIHIVQKGDTLWKIAKKYGVDFDTLKQTNTQLSNPDLIMPGMKIKVPSNGVQVKQHAGAGSAPPKQYVKEVQQKEFAAT
PTPLGIEDEEEVTYQSAPITQQPAMQQTQKEVQVKPQKEMQVKPQKEVQVKPQKEMQVKPQKEMQVKPQKEMQVKPQKEV
QVKPQKEVQKEKPIQIEKPSVIQKPPVIEKQKPAEKENTKFSVNVLPQPPQPPIKAKKEYKISDVIKKGSELIAPQITKM
KPNNIISPQTKKDNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNI
VSPQVKKENVGNIVSPNVSKENVVIPQVIPPNIQVPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQPPNMMPIMDN
NQMPNMMPIMDNNQPPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQMPNMMPIMDNNQMPNMMPIMDNNQMPNMMP
IMDNNQPPNMMPYQMPYQQPMMPPNPYYQQMPYQQGAPFGPQYTSMPNPNMMPMDNNMPPLVQGEEDCGCGGESRLYSPQ
PGGPQYANPLYYQPTQSAYAPQPGTMYYQPDPPNVFGEPVSEEEDEEEV
>Mature_609_residues
MKIHIVQKGDTLWKIAKKYGVDFDTLKQTNTQLSNPDLIMPGMKIKVPSNGVQVKQHAGAGSAPPKQYVKEVQQKEFAAT
PTPLGIEDEEEVTYQSAPITQQPAMQQTQKEVQVKPQKEMQVKPQKEVQVKPQKEMQVKPQKEMQVKPQKEMQVKPQKEV
QVKPQKEVQKEKPIQIEKPSVIQKPPVIEKQKPAEKENTKFSVNVLPQPPQPPIKAKKEYKISDVIKKGSELIAPQITKM
KPNNIISPQTKKDNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNI
VSPQVKKENVGNIVSPNVSKENVVIPQVIPPNIQVPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQPPNMMPIMDN
NQMPNMMPIMDNNQPPNMMPIMDNNQPPNIMPIMDNNQPPNIMPIMDNNQMPNMMPIMDNNQMPNMMPIMDNNQMPNMMP
IMDNNQPPNMMPYQMPYQQPMMPPNPYYQQMPYQQGAPFGPQYTSMPNPNMMPMDNNMPPLVQGEEDCGCGGESRLYSPQ
PGGPQYANPLYYQPTQSAYAPQPGTMYYQPDPPNVFGEPVSEEEDEEEV

Specific function: Required for the normal assembly and anchoring of both the spore coat and the exosporium layers [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis safA [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR014248 [H]

Pfam domain/function: PF01476 LysM [H]

EC number: NA

Molecular weight: Translated: 68730; Mature: 68730

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
8.9 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
8.9 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHIVQKGDTLWKIAKKYGVDFDTLKQTNTQLSNPDLIMPGMKIKVPSNGVQVKQHAGA
CEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCEEEECCCCCEEEECCCC
GSAPPKQYVKEVQQKEFAATPTPLGIEDEEEVTYQSAPITQQPAMQQTQKEVQVKPQKEM
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHCCCCHHHC
QVKPQKEVQVKPQKEMQVKPQKEMQVKPQKEMQVKPQKEVQVKPQKEVQKEKPIQIEKPS
CCCCCCCEECCCHHHCCCCCHHHCCCCCHHHCCCCCCCCEECCCHHHHHHCCCCEECCCC
VIQKPPVIEKQKPAEKENTKFSVNVLPQPPQPPIKAKKEYKISDVIKKGSELIAPQITKM
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCHHHCCHHEEE
KPNNIISPQTKKDNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPNVSKENVVIPQVIPPNIQVPNMMP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
IMDNNQPPNIMPIMDNNQPPNIMPIMDNNQPPNMMPIMDNNQMPNMMPIMDNNQPPNMMP
CCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCEECCCCCCCCCCEEE
IMDNNQPPNIMPIMDNNQPPNIMPIMDNNQMPNMMPIMDNNQMPNMMPIMDNNQMPNMMP
EECCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEECCCCCCCCEEC
IMDNNQPPNMMPYQMPYQQPMMPPNPYYQQMPYQQGAPFGPQYTSMPNPNMMPMDNNMPP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LVQGEEDCGCGGESRLYSPQPGGPQYANPLYYQPTQSAYAPQPGTMYYQPDPPNVFGEPV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCCCCCCC
SEEEDEEEV
CCCCCCCCC
>Mature Secondary Structure
MKIHIVQKGDTLWKIAKKYGVDFDTLKQTNTQLSNPDLIMPGMKIKVPSNGVQVKQHAGA
CEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCEEEECCCCCEEEECCCC
GSAPPKQYVKEVQQKEFAATPTPLGIEDEEEVTYQSAPITQQPAMQQTQKEVQVKPQKEM
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHCCCCHHHC
QVKPQKEVQVKPQKEMQVKPQKEMQVKPQKEMQVKPQKEVQVKPQKEVQKEKPIQIEKPS
CCCCCCCEECCCHHHCCCCCHHHCCCCCHHHCCCCCCCCEECCCHHHHHHCCCCEECCCC
VIQKPPVIEKQKPAEKENTKFSVNVLPQPPQPPIKAKKEYKISDVIKKGSELIAPQITKM
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCHHHCCHHEEE
KPNNIISPQTKKDNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPQVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KENVGNIVSPQVKKENVGNIVSPQVKKENVGNIVSPNVSKENVVIPQVIPPNIQVPNMMP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
IMDNNQPPNIMPIMDNNQPPNIMPIMDNNQPPNMMPIMDNNQMPNMMPIMDNNQPPNMMP
CCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCEECCCCCCCCCCEEE
IMDNNQPPNIMPIMDNNQPPNIMPIMDNNQMPNMMPIMDNNQMPNMMPIMDNNQMPNMMP
EECCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCCCEEEECCCCCCCCEEC
IMDNNQPPNMMPYQMPYQQPMMPPNPYYQQMPYQQGAPFGPQYTSMPNPNMMPMDNNMPP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LVQGEEDCGCGGESRLYSPQPGGPQYANPLYYQPTQSAYAPQPGTMYYQPDPPNVFGEPV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCCCCCCC
SEEEDEEEV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA