Click here to switch to the map view.

The map label for this gene is queA

Identifier: 49481504

GI number: 49481504

Start: 4214973

End: 4216025

Strand: Reverse

Name: queA

Synonym: BT9727_4151

Alternate gene names: 49481504

Gene position: 4216025-4214973 (Counterclockwise)

Preceding gene: 49480668

Following gene: 49481502

Centisome position: 80.49

GC content: 38.75

Gene sequence:

>1053_bases
ATGGATATTAATCTGTTTGATTTTCATTTACCAGAAGAACTTATTGCACAAGTCCCATTAGAAGAGCGTGAAACATCAAG
ATTAATGGTGTTAGACCGCGAAACAGGGGATATTGAGCATAAACATTTTACGGACATTCTTTCTTACTTACATGAGGGGG
ATTGCTTAGTTTTAAATGAAACGAAAGTAATGCCAGCTCGTTTGCACGGTGTGAAAGAAGATACAGGCGCACACATTGAA
GTACTTCTTTTAAAACAAGAGGAAGGCGATAAGTGGGAGACGCTTGTTAAGCCAGCGAAACGTGTAAAAGAAGGAACTGT
TATCTCTTTTGGCGAAGGGAAATTAAAAGCAACTTGCACAGGAACTGCAGATCAAGGTGGACGTCAGCTTGAATTTTCAT
ATGACGGCATCTTCTATGAAATTTTAGATGAACTTGGAGAAATGCCACTTCCTCCATATATTAAAGAGACGCTAGAAGAT
CGTGATCGCTATCAAACAGTATATGCGAAAGAAATCGGTTCAGCAGCAGCGCCGACTGCAGGACTTCACTTTACAGAAGA
GTTACTGGAGAAGTTAAAACAAAAAGGCGTTGAGTTAGCATTTATCACACTTCACGTAGGGCTTGGAACATTTAGACCAG
TTTCTGCTGATACGATTGAAGAGCACCATATGCATGCAGAATATTACCATATGTCTGAAGAGACAGCGGCATTATTAAAC
CGTGTAAAAGAGAATGGTGGACGTATTATTACGGTAGGTACGACTTCAACTCGTACGTTAGAAACAATTGCGACAGATCA
TGACGGCAAGCTTTGTGCTGCTTCTGGCTGGACAGATATTTTTATGTACCCAGGTTATGAATTTAAAGCAATTGATGGTT
TAATTACAAACTTCCATTTACCGAAATCAACATTAATTATGCTTGTAAGTGCATTTGCAAACAGAGATAATGTACTTCAT
GCTTATAATGAAGCAGTAAAAGAAAAATATCGTTTCTTTAGCTTCGGTGATGCAATGTTCGTTGCATCTCACGCTAAAAT
GGGAAACAAATAA

Upstream 100 bases:

>100_bases
TTTTATTTTCCTATCATTACATGTATTGTGTTAAGTATTGTATTATCTTTTATTATGTATATAATAAATCGATTCAAATA
AGAAATAGGTGGATATAGTT

Downstream 100 bases:

>100_bases
AAGGAGTAAATTATGACAGCAATTCGTTATGAATTTATTAAAACTTGTAAACAAACAGGTGCTCGTTTAGGACGTGTACA
TACGCCGCACGGTTCATTTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Sequences:

>Translated_350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK
>Mature_350_residues
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRDNVLH
AYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=47.7401129943503, Blast_Score=317, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACAA (C3P9A5)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868704.1
- ProteinModelPortal:   C3P9A5
- SMR:   C3P9A5
- EnsemblBacteria:   EBBACT00000126609
- GeneID:   7850828
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4665
- GeneTree:   EBGT00050000002867
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39387; Mature: 39387

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNE
CCCCEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TKVMPARLHGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHH
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHL
HHHHCCCEEEEEECCCCCEEHEEECCCCCCEEEECCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC
>Mature Secondary Structure
MDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNE
CCCCEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TKVMPARLHGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCT
CCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETAALLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHH
RVKENGGRIITVGTTSTRTLETIATDHDGKLCAASGWTDIFMYPGYEFKAIDGLITNFHL
HHHHCCCEEEEEECCCCCEEHEEECCCCCCEEEECCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA