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The map label for this gene is recJ

Identifier: 49481496

GI number: 49481496

Start: 4204281

End: 4206620

Strand: Reverse

Name: recJ

Synonym: BT9727_4142

Alternate gene names: 49481496

Gene position: 4206620-4204281 (Counterclockwise)

Preceding gene: 49481497

Following gene: 49478628

Centisome position: 80.31

GC content: 37.61

Gene sequence:

>2340_bases
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAAATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACAGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTTTGCAAGAATT
AGGTGCAGATGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTTCGTTGGGCGC
ATAGCGCAGGATTCTCATTAATTATTACTGTCGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTAATTATTACAGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCACCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTGGCTGATTTAGTATCACTTCATGGTGAGAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGTTGGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGAAAATTACAGAAGAAGCTATCGCCGAAGTGGAAACGAATTTTCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGATGGAATCCAGGTGTAATTGGAATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATAGCGGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCAGAGGTAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CTGTTGATGTTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATTGAAGATATGCAAAAGTTGGCGCCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATTGGATCCGACGGTTCACATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACATTGGATACAATTGGATTTGGATTTGGTGCGTATGCAAAAGAAATTTCTC
CAGTTGCAAAAGTCTCTGTAATTGGGGAAGCGTCTATTAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGCAGTATGCGTAACGTAGAAGCAAATGTGGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTGAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACAGAATTAGATGATCAATACATCGTGTTCCTTGATTTGCCAAAAGGAACAGATGAATTACGAGAG
TTATTTAAAGTAGGGTTCCCGTCGCGAATCTATACACTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGAGAACACTTTAAATGGTATTATTCGTTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAATAGAACAGTTAGGGTAA

Upstream 100 bases:

>100_bases
CATTATTAATATTTTAGTATATAGATATTGCATCGTAGTTTGCATTATATTGTCACCCCTTAATCTGTCTTGTATAATGA
ACAGGTTAAGGGGTGATTTC

Downstream 100 bases:

>100_bases
AGGATTTGTATTTACAAACCTTTTAGATCTGAGGAGGATTCAGAAATATGGATTTCAAGCAACATATCGCAATTGTACCG
GATTATCCGAAAGAAGGTAT

Product: single-stranded-DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKKITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLHKFSLEDYKEHMMHASEVTELDDQYIVFLDLPKGTDELRE
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLG

Sequences:

>Translated_779_residues
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKKITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLHKFSLEDYKEHMMHASEVTELDDQYIVFLDLPKGTDELRE
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLG
>Mature_779_residues
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKKITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLHKFSLEDYKEHMMHASEVTELDDQYIVFLDLPKGTDELRE
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLG

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=567, Percent_Identity=33.1569664902998, Blast_Score=260, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 88201; Mature: 88201

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDP
CCCCCCCCCHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
FLLEGMDRTVERVNKAIQNGEQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
EGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKELGIDLIITDHHEPPPELPEAL
CCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
AIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLL
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEEE
SEDPEEAKELAEEIDELNKLRKDIVKKITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHH
GIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLFANLSDCRELLPHFGGHPMAA
HHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCEEHHHHHCCHHHHHHHHHHCCCCCCCC
GMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
CEEEECCCHHHHHHHHCCCCEEEEECCCCCEEEHHEEEEEECCHHHHHHHHHHHHCCCCC
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSV
CCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCHHHEEE
IGEASINEWNNFKKPQLMVQDIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVL
ECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHEEEEEECHHHH
HKFSLEDYKEHMMHASEVTELDDQYIVFLDLPKGTDELRELFKVGFPSRIYTLFYQENNH
HHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCE
LFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
EEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEE
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDP
CCCCCCCCCHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
FLLEGMDRTVERVNKAIQNGEQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
EGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKELGIDLIITDHHEPPPELPEAL
CCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
AIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLL
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEEE
SEDPEEAKELAEEIDELNKLRKDIVKKITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHH
GIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLFANLSDCRELLPHFGGHPMAA
HHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCEEHHHHHCCHHHHHHHHHHCCCCCCCC
GMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
CEEEECCCHHHHHHHHCCCCEEEEECCCCCEEEHHEEEEEECCHHHHHHHHHHHHCCCCC
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSV
CCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCHHHEEE
IGEASINEWNNFKKPQLMVQDIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVL
ECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHEEEEEECHHHH
HKFSLEDYKEHMMHASEVTELDDQYIVFLDLPKGTDELRELFKVGFPSRIYTLFYQENNH
HHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCE
LFSTVPTREHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
EEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEE
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEIEQLG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]