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The map label for this gene is ackA

Identifier: 49481379

GI number: 49481379

Start: 4433992

End: 4435185

Strand: Reverse

Name: ackA

Synonym: BT9727_4371

Alternate gene names: 49481379

Gene position: 4435185-4433992 (Counterclockwise)

Preceding gene: 49480700

Following gene: 49480699

Centisome position: 84.68

GC content: 40.54

Gene sequence:

>1194_bases
ATGTCAAAAATCATCGCGATTAACGCAGGAAGCTCTTCCTTAAAATTCCAATTATTTGAAATGCCAAGTGAAACAGTATT
AACAAAAGGTTTAGTAGAACGTATCGGTTTAGAAGATAGTATCTTCACTATTACTGTAGATGGCGAAAAACAAAAAGAAA
TTACAAACATTCCAGATCACGCAGTAGCAGTTAATATGCTTCTTAAAAAATTAACTGAAAACGGAATCGTTAAATCTCTA
GATGAGATTGGCGGTATCGGTCACCGTGTTGTTCACGGCGGCGAAAAATTTGCTGACTCTGTTCTAATTACTGATGAAGT
ATTAGCTGATATCGAAGAATTAAGCGATTTAGCACCACTTCATAACCCAGCAAACGTTGTTGGTATTAAAGCATTCCAAG
AAGTATTACCAAACGTACCAGCAGTAGCAGTATTCGATACAGCATTCCACCAAACAATGCCGGAATCTGCATTCCTATAC
AGCTTACCGTATGAGTACTACGAAAAGTTCGGTATCCGTAAGTACGGTTTCCATGGAACTTCTCATAAATACGTAACTGA
GCGTGCGGCTGAACTATTAGGTCGTCCACTTGAGAGCTTAAGCTTACTTTCTTGTCACTTAGGTAACGGTGCAAGTATCG
CAGCAGTAGAAGGCGGTAAATCTATCGATACTTCTATGGGCTTCACTCCACTTGCTGGTGTAACAATGGGTACACGTTCT
GGTAACATTGACCCTGCGTTAATTCCATACATCATGGAAAAAACAGGCCAAACAGTAGAAGAAGTAGTTAGCGTATTAAA
CAAGAAGAGTGGTATGTTAGGTCTTACTGGTTACTCTAGTGACCTACGTGACATCATTGCGAAAGAAGAAGAAGGCGACC
ACCGTGCGAAAGTAGCACTTGATGTATTCGTAAGCCGTATCCACAAATACATCGGTTCTTACACTGCTCGTATGAAAGGT
GTAGACGCAATCATCTTCACAGCTGGTGTAGGTGAAAACAGTGCGATTATTCGTGAACGCGTATTAGAAGGCCTTGAGTA
CATGGGCGTATACTTCGATGCAAAACGCAATAACGTATTCGGTGAGGAAGCATTCATCAACTTCCCACATTCTCCAGTAA
AAATTATTGTAATTCCAACTGACGAAGAAGTTATGATCGCTCGTGACGTACTACGTCTTGGAAACATCGATTAA

Upstream 100 bases:

>100_bases
GATGAAACGTTTTCATATTTAGTAAATTTGATCAGATAAAATCGAGCGGTTTGTGGAAACATCCACACAACTACCAAGAG
AAAAAGGGGCGAAAGACTAG

Downstream 100 bases:

>100_bases
TATATGAAAAGAAAGACGAAGTCCTATAGGATTTCGTCTTTTTTCTTTTTGATGTATGTTAAAATTCCATCTGGATATAG
TACTTTTTTCTCCTGAATGA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSL
DEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQTVEEVVSVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKG
VDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNID

Sequences:

>Translated_397_residues
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSL
DEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLY
SLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
GNIDPALIPYIMEKTGQTVEEVVSVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKG
VDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNID
>Mature_396_residues
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDHAVAVNMLLKKLTENGIVKSLD
EIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPLHNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYS
LPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRSG
NIDPALIPYIMEKTGQTVEEVVSVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVALDVFVSRIHKYIGSYTARMKGV
DAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVFGEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNID

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=44.6115288220551, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=43.5768261964735, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43263; Mature: 43132

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDH
CCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLKKLTENGIVKSLDEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPL
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHCCC
HNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYSLPYEYYEKFGIRKYGFHGT
CCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQTVEEVVSVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYTARMKGVDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNID
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGLEDSIFTITVDGEKQKEITNIPDH
CEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCHH
AVAVNMLLKKLTENGIVKSLDEIGGIGHRVVHGGEKFADSVLITDEVLADIEELSDLAPL
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCHHHHCCEEEHHHHHHHHHHHHHHCCC
HNPANVVGIKAFQEVLPNVPAVAVFDTAFHQTMPESAFLYSLPYEYYEKFGIRKYGFHGT
CCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEEECCHHHHHHCCCEEECCCCC
SHKYVTERAAELLGRPLESLSLLSCHLGNGASIAAVEGGKSIDTSMGFTPLAGVTMGTRS
CCHHHHHHHHHHHCCCHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCC
GNIDPALIPYIMEKTGQTVEEVVSVLNKKSGMLGLTGYSSDLRDIIAKEEEGDHRAKVAL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHH
DVFVSRIHKYIGSYTARMKGVDAIIFTAGVGENSAIIRERVLEGLEYMGVYFDAKRNNVF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCC
GEEAFINFPHSPVKIIVIPTDEEVMIARDVLRLGNID
CCHHEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]