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The map label for this gene is rpi

Identifier: 49481302

GI number: 49481302

Start: 5053178

End: 5053621

Strand: Reverse

Name: rpi

Synonym: BT9727_5000

Alternate gene names: 49481302

Gene position: 5053621-5053178 (Counterclockwise)

Preceding gene: 49480411

Following gene: 49478914

Centisome position: 96.49

GC content: 39.64

Gene sequence:

>444_bases
ATGAAAGTAGTAATAGCATCTGATCACGGCGGTATGAATATCCGTAAAGAACTTGTAAGTTTATTAGAAGAGTTAAATGT
TGAATATATTGATTTAGGTTGTGAATGTGAAGCTGGTTCTGTTGATTACCCTGATTTTGCGTTTCCAGCAGCGGAAATGG
TAGCAAATGGCGAAGTAGACCGTGGTATTTTAGTTTGTGGGACAGGCATCGGTATGTCAATTGCTGCAAACAAAGTAAAT
GGTATTCGTTGTGCGTTAGTTCATGATACTTTCAGTGCGAGAGCAACAAGAGAGCATAATGATACAAACATGTTAGCAAT
GGGCGAGCGTGTAATTGGTGCTGGTCTAGCACGTGACATCGCAAAAATTTGGTTAACAACTGAATTTGAAGGTGGCCGTC
ACGAAAACCGCGTAGGAAAAATTAAAACATACGAAGCAAAGTAA

Upstream 100 bases:

>100_bases
TTGATGTTTCGTGTATAATACGATATTGGAGATCACTTCGTTCCATTACAGGAGTGATTGAGAAATGAAGATAATGGATT
GAAACTTTGGAGGGGTAAAA

Downstream 100 bases:

>100_bases
TAGATTCTAACTAGAAGCAGTAGGAAATATAATTTGGTGAACCAACCTGTGAAAGGAAGAGGCGTAATGACAGAAATCGT
AAAGGTAAGAGAACAGCTAC

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKVVIASDHGGMNIRKELVSLLEELNVEYIDLGCECEAGSVDYPDFAFPAAEMVANGEVDRGILVCGTGIGMSIAANKVN
GIRCALVHDTFSARATREHNDTNMLAMGERVIGAGLARDIAKIWLTTEFEGGRHENRVGKIKTYEAK

Sequences:

>Translated_147_residues
MKVVIASDHGGMNIRKELVSLLEELNVEYIDLGCECEAGSVDYPDFAFPAAEMVANGEVDRGILVCGTGIGMSIAANKVN
GIRCALVHDTFSARATREHNDTNMLAMGERVIGAGLARDIAKIWLTTEFEGGRHENRVGKIKTYEAK
>Mature_147_residues
MKVVIASDHGGMNIRKELVSLLEELNVEYIDLGCECEAGSVDYPDFAFPAAEMVANGEVDRGILVCGTGIGMSIAANKVN
GIRCALVHDTFSARATREHNDTNMLAMGERVIGAGLARDIAKIWLTTEFEGGRHENRVGKIKTYEAK

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=146, Percent_Identity=47.2602739726027, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15928; Mature: 15928

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVIASDHGGMNIRKELVSLLEELNVEYIDLGCECEAGSVDYPDFAFPAAEMVANGEVD
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCC
RGILVCGTGIGMSIAANKVNGIRCALVHDTFSARATREHNDTNMLAMGERVIGAGLARDI
CCEEEEECCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHH
AKIWLTTEFEGGRHENRVGKIKTYEAK
HHHEEEECCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKVVIASDHGGMNIRKELVSLLEELNVEYIDLGCECEAGSVDYPDFAFPAAEMVANGEVD
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCC
RGILVCGTGIGMSIAANKVNGIRCALVHDTFSARATREHNDTNMLAMGERVIGAGLARDI
CCEEEEECCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHH
AKIWLTTEFEGGRHENRVGKIKTYEAK
HHHEEEECCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]