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The map label for this gene is pstA

Identifier: 49481279

GI number: 49481279

Start: 4075003

End: 4075869

Strand: Reverse

Name: pstA

Synonym: BT9727_4010

Alternate gene names: 49481279

Gene position: 4075869-4075003 (Counterclockwise)

Preceding gene: 49481281

Following gene: 49481277

Centisome position: 77.82

GC content: 34.37

Gene sequence:

>867_bases
ATGCGAATGTTGAATCATAAAAAAATACAAGAGAATATGGCATCACGGTTTTTAAAAGATCGAATTTACAAATTGTTATT
TTATATAGCAATTTTGTTTTCAATAGTAATACTACTTATTTTGCTTTTTCAAATTTTTGAAAAAGGTATCAGTTACCTTT
CGTTAGAGTTTTTTACAAACTTCGCTTCGCGTAATCCGAAAGAAGCTGGGATTGCTGCAGCATTGTCAGGAACAATATTA
TTTATGAGTATTGTTATACCAGTCTCCTTTATATTTGGAGTCGGGACTGCTCTTTATTTAGAACAATATGCGAAGGAATC
TATATTTAAAAAGGTAATAGAAATTAATAATCAAACGTTAGCAGGAGTACCTTCCGTTGTATTTGGATTACTCGGATTAA
CTATTTTTGTATATGCTCTTCATTTAGGTGAGAGTATTATTGCGGCGGCACTGACGATGAGTTTACTCGTCTTACCTACA
GTTGTTGTAGCAAGTCAGGAAGCGATACGCTCAGTACCAAATTCATTATTAGAAGCTTCTTACGGATTAGGTGCTACGAA
GTGGCAAACGATGTATCGAATTGTATTGCCGTATGCTTTCCCAGGAATTATAACAGGTTGTACGCTAGCGATATCAAGAG
CGATTGGAGAAGCGGCACCGTTATTAGTTATTGGGGCACTTGCATTTGCAAATTATGTTCCATTTAGTATGTTTGATAGA
TTTACAGTTTTACCAATTCAAATTTTTAATTGGATGAGTAGACCACAAGAAGAATTTCAGTATGTAGCAGCTGCTGGGAT
GATTGTTTTGTTAGGTTTATTGCTCTTGATCAATATATTTGTCCTATGGTTACGAAATCGAAAATAA

Upstream 100 bases:

>100_bases
TACAGCATGTACGCTGTAGGCGCGACGTTATTCTTATTTACTTTTATAATGAACATCATGTCTCAGTTTATTATGCGCCG
CTTTAGGAGGGTGACATAAT

Downstream 100 bases:

>100_bases
GTTGGAAATGGATGAAAGGGGAATTCAAATGGTGGCAACAGTAGTAAATGTACAGGTGAAAAACGAGGAGAAAATTGAGA
CTGCGCCAAAGAAAGTAGTA

Product: phosphate ABC transporter permease

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRMLNHKKIQENMASRFLKDRIYKLLFYIAILFSIVILLILLFQIFEKGISYLSLEFFTNFASRNPKEAGIAAALSGTIL
FMSIVIPVSFIFGVGTALYLEQYAKESIFKKVIEINNQTLAGVPSVVFGLLGLTIFVYALHLGESIIAAALTMSLLVLPT
VVVASQEAIRSVPNSLLEASYGLGATKWQTMYRIVLPYAFPGIITGCTLAISRAIGEAAPLLVIGALAFANYVPFSMFDR
FTVLPIQIFNWMSRPQEEFQYVAAAGMIVLLGLLLLINIFVLWLRNRK

Sequences:

>Translated_288_residues
MRMLNHKKIQENMASRFLKDRIYKLLFYIAILFSIVILLILLFQIFEKGISYLSLEFFTNFASRNPKEAGIAAALSGTIL
FMSIVIPVSFIFGVGTALYLEQYAKESIFKKVIEINNQTLAGVPSVVFGLLGLTIFVYALHLGESIIAAALTMSLLVLPT
VVVASQEAIRSVPNSLLEASYGLGATKWQTMYRIVLPYAFPGIITGCTLAISRAIGEAAPLLVIGALAFANYVPFSMFDR
FTVLPIQIFNWMSRPQEEFQYVAAAGMIVLLGLLLLINIFVLWLRNRK
>Mature_288_residues
MRMLNHKKIQENMASRFLKDRIYKLLFYIAILFSIVILLILLFQIFEKGISYLSLEFFTNFASRNPKEAGIAAALSGTIL
FMSIVIPVSFIFGVGTALYLEQYAKESIFKKVIEINNQTLAGVPSVVFGLLGLTIFVYALHLGESIIAAALTMSLLVLPT
VVVASQEAIRSVPNSLLEASYGLGATKWQTMYRIVLPYAFPGIITGCTLAISRAIGEAAPLLVIGALAFANYVPFSMFDR
FTVLPIQIFNWMSRPQEEFQYVAAAGMIVLLGLLLLINIFVLWLRNRK

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=279, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790164, Length=159, Percent_Identity=30.188679245283, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32036; Mature: 32036

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]