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The map label for this gene is nuoM

Identifier: 49481258

GI number: 49481258

Start: 5028215

End: 5029717

Strand: Reverse

Name: nuoM

Synonym: BT9727_4973

Alternate gene names: 49481258

Gene position: 5029717-5028215 (Counterclockwise)

Preceding gene: 49481259

Following gene: 49480403

Centisome position: 96.03

GC content: 41.72

Gene sequence:

>1503_bases
ATGAATGATTTGTTATTAACGTTCTTCATTTTTTCCCCGCTTTTAGGAATTCTCTTACTTGCATTAACACCGAAAAAAGA
ATCGCGTACAGTGCGAGCGCTCGGTTTATTTGGAACAGTGCTTCCATTTGGAATGGCTATCGTCCTCGCTTGTACATATG
CTTCTGGAAGGGATTTGTCTCTATTTGATGAAAAAGTGAAATGGATTAAATTCGGGGATTTTGCAGCGATAGATAAAAGG
TGGTTTTCTATTTATTACGAACTTGGTATAGATGGTTTATCGCTCGTTATGATGGTGCTGACGGCCCTTCTAGCGATGCT
TGCAGCAATTGCGGCATTTACGATTAAAAGAAATTTGAAAGCATTTTATATGCTGTTACTCATGTTAGAAATAGGGATGC
TCGGCGTCTTCGCTGCTCAAAATTTAATGTTGTTCTTTATCTTCTTTGAAATTACACTGCCGCCGATGTTTTTATTAATT
GGGAAGTGGGGGAAATTGTCGAGTGAAAAGGCTGCTTATAGTTATTTAATATATAACGGTATTGGCTCCGCTATTTTACT
CATCGTTTTCTCGGTTTTATTTGCGAAAACAGGTACAACAAATATTACAGAACTGAAAGAAATATTAGCGAGTGTAGGCG
CTGGGGGAGGAATGGTTGTCCCAAGTAGCTTACAGTTCGGCTTGTTTCTTGCCATAATGATTGCTTTCGCAATTAAATTA
CCCGTTTTCCCGTTACATCGCTGGATGGTCAATGTACATATTGAAGCGCATCCGGCTGTAGTGATGCTTCATGCAGGTGT
TTTACTGAAGATTGGAGCATACGGTATCATTCGCTTCGGGCAGGGGCTATTCCCGGAATACTTTCGCGAGTTTGCAACGC
TCATTGCGATTTTAGGAGTTATCAATTTATTGTACGGAGCCTTCTTAGCACTCATTCAAACAGACTTTCGGAAGGTGCTT
GCTTACTCTAGTATTTCGCATATGGGTATTGTATTAATGGGCCTTGCAGCGTTAAACGCACCAGGTACACAAGGGGCATT
ATTCCAAGTTGTGTCGCATGGCTTAATTGCAGCCTTACTCTTTTTCTTACTTGGTGTTATAGAACAGCGCTTTGGAACGT
CGGATATTACAGCGCTTGGCGGACTTGCAAAAAGTGTACCAGTACTTAGCGGTTTCTTCTTGGCGGGAGGAATGGCATCG
CTTGGATTGCCGGGAATGTCTGGATTTGTTAGCGAGTTTCTTGCCTTTCTCGGTTTATTCCAAGTAGAACCAGTCATTGC
TGCGGCCGGAGTACTTGGCATCATTTTAACCGCTGTATACGTATTAAGAGCAACCCTTCAAGTAACATTTGGTAAGAAAG
AGTGGGAAGCGAAAGCTGATATACACGGATGGGAGTATGTTCCTATCTTGCTACTTATCTTCTGCATTATTGCAATTGGC
GTAATGCCAGAAATACTAGGGGATCCGCTTCAAAATACATTGAAAACATTGGGGGTGAAGTAG

Upstream 100 bases:

>100_bases
CTAGACTCCAAAACGGAAATGTACAAGTGTACGGGACTGTAACAGCTGTTTCACTTGCAGTACTCTTCATTATTTTGCTC
TATACGGGAGGGGATTTACG

Downstream 100 bases:

>100_bases
GATGGATATGAATACGTTACTTAGCTTATCGTGGCATCTCATGGTGCCAGAATTCATCATTCTCGGGGCTGCCATCCTTC
TTTCCATATGTGATTTGTTT

Product: NADH dehydrogenase subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAIDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQVEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK

Sequences:

>Translated_500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAIDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQVEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK
>Mature_500_residues
MNDLLLTFFIFSPLLGILLLALTPKKESRTVRALGLFGTVLPFGMAIVLACTYASGRDLSLFDEKVKWIKFGDFAAIDKR
WFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFTIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLI
GKWGKLSSEKAAYSYLIYNGIGSAILLIVFSVLFAKTGTTNITELKEILASVGAGGGMVVPSSLQFGLFLAIMIAFAIKL
PVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGQGLFPEYFREFATLIAILGVINLLYGAFLALIQTDFRKVL
AYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMAS
LGLPGMSGFVSEFLAFLGLFQVEPVIAAAGVLGIILTAVYVLRATLQVTFGKKEWEAKADIHGWEYVPILLLIFCIIAIG
VMPEILGDPLQNTLKTLGVK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI251831117, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788613, Length=463, Percent_Identity=35.85313174946, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788614, Length=512, Percent_Identity=28.125, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=28.525641025641, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=283, Percent_Identity=29.3286219081272, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788829, Length=428, Percent_Identity=23.1308411214953, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI2367154, Length=354, Percent_Identity=25.7062146892655, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI145693160, Length=403, Percent_Identity=24.8138957816377, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54136; Mature: 54136

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]