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The map label for this gene is celF

Identifier: 49481176

GI number: 49481176

Start: 4934949

End: 4936274

Strand: Reverse

Name: celF

Synonym: BT9727_4886

Alternate gene names: 49481176

Gene position: 4936274-4934949 (Counterclockwise)

Preceding gene: 49481178

Following gene: 49481174

Centisome position: 94.25

GC content: 38.01

Gene sequence:

>1326_bases
ATGACTGGAATTAAAATTGCTACAATCGGCGGTGGATCTAGTTATACACCAGAGTTAATTGAAGGATTTATTAAACGTTA
TGATGAGCTTCCTGTTCGTGAAATTTGGTTAGTAGATATTGAGGCAGGAAAAGAAAAGTTAGAAATCGTTGGTAACTTAG
CGAAACGTATGGTGAAAAAATCTGGTTTACCAATTGAGGTACATTTAACGCTTGATCGTCGCGAAGCATTAAAAGATGCT
GACTTCGTAACGACGCAGCTTCGCGTAGGTTTATTAGAAGCTCGCGCAAAAGATGAAGCAATCCCGTTAAAATATGATGT
AATCGGTCAGGAAACGAATGGTCCTGGTGGTTTATTCAAAGCATTGAGAACGATTCCTGTTATTTTAGATATTTGTAAGG
ACATGGAGGAGCTTTGTCCGAATGCATGGTTAATTAACTTTGCAAACCCAGCTGGTATGGTAACAGAAGCAGTCCTTCGT
TATACAAATATTCAAAGAGTAGTTGGTCTATGTAACGTTCCAATCGGAATTCGTATGGGTCTTGCAAAATTACTTGAAGT
AGATGCAAGTCGTGTACACGTTGACTTCGCAGGTTTAAACCATATGGTATACGGACTAGACGTATACTTAGATGGCGTAA
GTGTAATGGATCGTGTGTTAGAACTTGTAACAGATCCAGAAAAGCAAATTACGATGGAAAATATCGCAGCGCTTAACTGG
GAACCAGACTTTATTCGTGGCCTTCGTGCAATTCCATGTCCATATCACCGTTACTACTACAAAACACGTGAAATGTTAGA
AGAAGAGAAAGAAGCTTCGGTTGAAAAAGGTACACGTGCAGAAGTAGTAAAACAATTAGAAGATGATTTATTCGAGTTAT
ATAAAGACCCGAACTTAGATATTAAACCACCACAATTAGAAAAACGTGGAGGTGCTTATTATAGTGATGCAGCATGTAGC
TTAATTACGTCTATTTACAATAATAAAGGTGATATCCAGCCTGTTAATACACGAAACAACGGAACAATTGCAAGCTTACC
ACATGATTCTGCTGTTGAAGTGAACTGTATTATTACGAAAGAAGGTCCAAAACCAATTGCAGTTGGAGATCTTCCAGTAC
CAGTTCGCGGTTTAGTACAACAAATTAAATCATTTGAGCGCACAACAATTGAAGCTGCTGTTACAGGGGATTATCATAAA
GCGCTGCTTGCTATGACAATTAACCCACTTGTACCATCAGATAAAGTTGCAAAACAAATTTTAGATGAAATGTTGGAAGC
GCATAAAGAATATCTTCCGCAGTTCTTCAAAAAGGTAGAGAAATAA

Upstream 100 bases:

>100_bases
TCACGCACAAGATCATTTAATGAATGCGATTACAGTAAAAGAATTAGCGGCAGAGTTTATCGATCTTTATAAAAAATTAG
AAGCGAAAGGGGAATAAAGC

Downstream 100 bases:

>100_bases
AGCGGCGCTATTTAGCCCGCTTTTTTTATTCTATTAGGAGGTATTATGATGATTAAGTTAATTGTAAATGCAGATGATTT
CGGTCTTACAGAAGGTACAA

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKSGLPIEVHLTLDRREALKDA
DFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLR
YTNIQRVVGLCNVPIGIRMGLAKLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPEKQITMENIAALNW
EPDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEKGTRAEVVKQLEDDLFELYKDPNLDIKPPQLEKRGGAYYSDAACS
LITSIYNNKGDIQPVNTRNNGTIASLPHDSAVEVNCIITKEGPKPIAVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHK
ALLAMTINPLVPSDKVAKQILDEMLEAHKEYLPQFFKKVEK

Sequences:

>Translated_441_residues
MTGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKSGLPIEVHLTLDRREALKDA
DFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLR
YTNIQRVVGLCNVPIGIRMGLAKLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPEKQITMENIAALNW
EPDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEKGTRAEVVKQLEDDLFELYKDPNLDIKPPQLEKRGGAYYSDAACS
LITSIYNNKGDIQPVNTRNNGTIASLPHDSAVEVNCIITKEGPKPIAVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHK
ALLAMTINPLVPSDKVAKQILDEMLEAHKEYLPQFFKKVEK
>Mature_440_residues
TGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKSGLPIEVHLTLDRREALKDAD
FVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLRY
TNIQRVVGLCNVPIGIRMGLAKLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPEKQITMENIAALNWE
PDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEKGTRAEVVKQLEDDLFELYKDPNLDIKPPQLEKRGGAYYSDAACSL
ITSIYNNKGDIQPVNTRNNGTIASLPHDSAVEVNCIITKEGPKPIAVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHKA
LLAMTINPLVPSDKVAKQILDEMLEAHKEYLPQFFKKVEK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=435, Percent_Identity=51.9540229885057, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1790560, Length=467, Percent_Identity=27.6231263383298, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49259; Mature: 49127

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKK
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
SGLPIEVHLTLDRREALKDADFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFK
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
ALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLRYTNIQRVVGLCNVPIGIRMG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAKLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPEKQITMENIAALNW
HHHHHHCCCHHEEEEHHCCCHHHEEHHHEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EPDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEKGTRAEVVKQLEDDLFELYKDPNLD
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSDAACSLITSIYNNKGDIQPVNTRNNGTIASLPHDSAVEVNCIITK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCCCCEEEEEEEEC
EGPKPIAVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHKALLAMTINPLVPSDKVAKQI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHEECCCCCCHHHHHHH
LDEMLEAHKEYLPQFFKKVEK
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKK
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
SGLPIEVHLTLDRREALKDADFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFK
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
ALRTIPVILDICKDMEELCPNAWLINFANPAGMVTEAVLRYTNIQRVVGLCNVPIGIRMG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LAKLLEVDASRVHVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPEKQITMENIAALNW
HHHHHHCCCHHEEEEHHCCCHHHEEHHHEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EPDFIRGLRAIPCPYHRYYYKTREMLEEEKEASVEKGTRAEVVKQLEDDLFELYKDPNLD
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSDAACSLITSIYNNKGDIQPVNTRNNGTIASLPHDSAVEVNCIITK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCCCCEEEEEEEEC
EGPKPIAVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHKALLAMTINPLVPSDKVAKQI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHEECCCCCCHHHHHHH
LDEMLEAHKEYLPQFFKKVEK
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]