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The map label for this gene is bioA

Identifier: 49480907

GI number: 49480907

Start: 3944753

End: 3946141

Strand: Reverse

Name: bioA

Synonym: BT9727_3861

Alternate gene names: 49480907

Gene position: 3946141-3944753 (Counterclockwise)

Preceding gene: 49480613

Following gene: 49478539

Centisome position: 75.34

GC content: 37.08

Gene sequence:

>1389_bases
GTGACTTTTAGTAAGAGTACGACTGACAAACCATCTTATACATATGAAGAGTTATCGGAAAAAAATAAAGCCTATGTATG
GCACCCATTTACACAAATGAAGGATTATTTAGAAGAAGATCCTGTCATTATTGAACGTGGAGAGGGAAGAAAGCTATATG
ATGTAAATGGAAATGAATATTGGGACGGTGTTTCGTCTATTTGGTTAAATGTTCATGGGCATCAAGTGCCAGAATTAGAT
GAGGCAATTCGTGAGCAATTAAATAAAATTGCCCATTCTACTATGTTAGGGCTTGCTAACGTTCCGTCTATTTTATTAGC
AGAAAAAATAATTGAAGTTGTACCAGAAGGCTTGAAAAAAGTATTCTATTCGGACTCCGGTTCTAGCGCTGTTGAAATTG
CAATTAAGATGGCCTTTCAATATTGGCAGCATAAAGGAAAACCGAAAAAACAAAGATTTGTTACATTAAAAGAAGCGTAT
CACGGTGATACAATTGGAGCTGTTTCAGTAGGAGCAATAGACTTGTTTCACCAAGTGTATAGTTCACTTTTATTTGAGGC
AATTAAAATGCCTTATCCATATACATATCGTTCACCTTATGGAAATGATAAGGCGGAAATTGTAAAAAAACATTTAGAAG
AAATGGAAGAGCTACTGAAAGATAAACATGAAGAAATTGCGGCGATTATTGTAGAACCTTTAATGCAAGGTGCTGGCGGT
ATGATTACAATGCCAAAAGGATACTTAAGAGGGCTACGTAATTTATGTACAAAATATAACGTTTTATTTATTACAGATGA
GGTAGCGACTGGGTTTGGGCGTACTGGGAAAATGTTTGCATGTGAACATGAAAATGTAACGCCGGATATTTTGACAGCCG
GAAAAGGTTTAACGGGCGGATATTTACCAGTTGCAATCACTGTAACGACAGATGAAATTTATAATGCCTTTTTAGGGAGC
TATGAAGAACAAAAAACGTTTTTCCACGGTCATAGTTATACAGGGAATCCGTTAGGTTGTGCGGTAGCAATTGCGAATCT
AGAACTATACGAGAAAACAAATTTAATAGAAGAAGTGGCACGTAAAACAGAATATGTGGCGACACAATTAGAAGAGCTCT
TTGCATGTAAACATGTAGGGGATATTCGTCAGTGTGGGTTAATGGTTGGAATTGAACTTGTGAAGAATAAAGAAACGAAA
GAAGCGTTTGAATGGACAGAGAGAGTCGGTGTTCAAGTATGTAAACGCTCAAGAGAGCTGGGTATGATTTTGCGCCCGCT
CGGTAACACGATTGTATTTATGCCTCCCCTTGCTTCTACAATTGATGAGATTGATGAGATGTTACGTATTTTATATAAAG
CAATCTCGGATATTACGGAGGGAGAATAA

Upstream 100 bases:

>100_bases
TGTTCTTTTTTAAGAACTACTTGATTTAAAAAGAATTGACAGTCATTTAGATTTAATGTTAACTATATTTATAAACTAGT
TAACATTAGGGGTGACAATT

Downstream 100 bases:

>100_bases
TGAGCGGTTTTTTTATAACAGCAACGGATACTGAAGTGGGAAAAACTGTAGTTGCAGGTGCATTAGCAGGTGTATTTAGA
GAATTGGGATATAACATCGG

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTFSKSTTDKPSYTYEELSEKNKAYVWHPFTQMKDYLEEDPVIIERGEGRKLYDVNGNEYWDGVSSIWLNVHGHQVPELD
EAIREQLNKIAHSTMLGLANVPSILLAEKIIEVVPEGLKKVFYSDSGSSAVEIAIKMAFQYWQHKGKPKKQRFVTLKEAY
HGDTIGAVSVGAIDLFHQVYSSLLFEAIKMPYPYTYRSPYGNDKAEIVKKHLEEMEELLKDKHEEIAAIIVEPLMQGAGG
MITMPKGYLRGLRNLCTKYNVLFITDEVATGFGRTGKMFACEHENVTPDILTAGKGLTGGYLPVAITVTTDEIYNAFLGS
YEEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNLIEEVARKTEYVATQLEELFACKHVGDIRQCGLMVGIELVKNKETK
EAFEWTERVGVQVCKRSRELGMILRPLGNTIVFMPPLASTIDEIDEMLRILYKAISDITEGE

Sequences:

>Translated_462_residues
MTFSKSTTDKPSYTYEELSEKNKAYVWHPFTQMKDYLEEDPVIIERGEGRKLYDVNGNEYWDGVSSIWLNVHGHQVPELD
EAIREQLNKIAHSTMLGLANVPSILLAEKIIEVVPEGLKKVFYSDSGSSAVEIAIKMAFQYWQHKGKPKKQRFVTLKEAY
HGDTIGAVSVGAIDLFHQVYSSLLFEAIKMPYPYTYRSPYGNDKAEIVKKHLEEMEELLKDKHEEIAAIIVEPLMQGAGG
MITMPKGYLRGLRNLCTKYNVLFITDEVATGFGRTGKMFACEHENVTPDILTAGKGLTGGYLPVAITVTTDEIYNAFLGS
YEEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNLIEEVARKTEYVATQLEELFACKHVGDIRQCGLMVGIELVKNKETK
EAFEWTERVGVQVCKRSRELGMILRPLGNTIVFMPPLASTIDEIDEMLRILYKAISDITEGE
>Mature_461_residues
TFSKSTTDKPSYTYEELSEKNKAYVWHPFTQMKDYLEEDPVIIERGEGRKLYDVNGNEYWDGVSSIWLNVHGHQVPELDE
AIREQLNKIAHSTMLGLANVPSILLAEKIIEVVPEGLKKVFYSDSGSSAVEIAIKMAFQYWQHKGKPKKQRFVTLKEAYH
GDTIGAVSVGAIDLFHQVYSSLLFEAIKMPYPYTYRSPYGNDKAEIVKKHLEEMEELLKDKHEEIAAIIVEPLMQGAGGM
ITMPKGYLRGLRNLCTKYNVLFITDEVATGFGRTGKMFACEHENVTPDILTAGKGLTGGYLPVAITVTTDEIYNAFLGSY
EEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNLIEEVARKTEYVATQLEELFACKHVGDIRQCGLMVGIELVKNKETKE
AFEWTERVGVQVCKRSRELGMILRPLGNTIVFMPPLASTIDEIDEMLRILYKAISDITEGE

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=455, Percent_Identity=32.7472527472527, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI37574042, Length=443, Percent_Identity=30.9255079006772, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI226442705, Length=443, Percent_Identity=30.6997742663657, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI226442709, Length=404, Percent_Identity=31.4356435643564, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI24119277, Length=436, Percent_Identity=29.3577981651376, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI284507298, Length=334, Percent_Identity=34.7305389221557, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI13994255, Length=449, Percent_Identity=27.1714922048998, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI188536080, Length=293, Percent_Identity=27.3037542662116, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI38679950, Length=293, Percent_Identity=27.3037542662116, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI38679946, Length=293, Percent_Identity=27.3037542662116, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786991, Length=439, Percent_Identity=35.7630979498861, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI145693181, Length=420, Percent_Identity=32.1428571428571, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788044, Length=415, Percent_Identity=33.2530120481928, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789759, Length=416, Percent_Identity=30.5288461538462, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789016, Length=427, Percent_Identity=28.1030444964871, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1787560, Length=423, Percent_Identity=29.5508274231678, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786349, Length=337, Percent_Identity=28.486646884273, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=456, Percent_Identity=30.7017543859649, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32564660, Length=449, Percent_Identity=29.6213808463252, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=251, Percent_Identity=35.8565737051793, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=447, Percent_Identity=27.5167785234899, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17541228, Length=362, Percent_Identity=25.9668508287293, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324386, Length=483, Percent_Identity=31.2629399585921, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6324432, Length=442, Percent_Identity=30.0904977375566, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323470, Length=449, Percent_Identity=27.8396436525612, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321456, Length=363, Percent_Identity=28.099173553719, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357415, Length=438, Percent_Identity=30.5936073059361, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI161085790, Length=442, Percent_Identity=29.4117647058824, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21356575, Length=432, Percent_Identity=29.6296296296296, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI28574759, Length=444, Percent_Identity=29.2792792792793, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI281366494, Length=311, Percent_Identity=27.6527331189711, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24667139, Length=311, Percent_Identity=27.6527331189711, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24667143, Length=311, Percent_Identity=27.6527331189711, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 52099; Mature: 51968

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSKSTTDKPSYTYEELSEKNKAYVWHPFTQMKDYLEEDPVIIERGEGRKLYDVNGNEY
CCCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCEEEECCCCCEEEECCCCCH
WDGVSSIWLNVHGHQVPELDEAIREQLNKIAHSTMLGLANVPSILLAEKIIEVVPEGLKK
HCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFYSDSGSSAVEIAIKMAFQYWQHKGKPKKQRFVTLKEAYHGDTIGAVSVGAIDLFHQVY
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SSLLFEAIKMPYPYTYRSPYGNDKAEIVKKHLEEMEELLKDKHEEIAAIIVEPLMQGAGG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MITMPKGYLRGLRNLCTKYNVLFITDEVATGFGRTGKMFACEHENVTPDILTAGKGLTGG
EEECCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCCCC
YLPVAITVTTDEIYNAFLGSYEEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNLIEEVA
CEEEEEEEEHHHHHHHHHCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKTEYVATQLEELFACKHVGDIRQCGLMVGIELVKNKETKEAFEWTERVGVQVCKRSREL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GMILRPLGNTIVFMPPLASTIDEIDEMLRILYKAISDITEGE
HHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFSKSTTDKPSYTYEELSEKNKAYVWHPFTQMKDYLEEDPVIIERGEGRKLYDVNGNEY
CCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCCEEEECCCCCEEEECCCCCH
WDGVSSIWLNVHGHQVPELDEAIREQLNKIAHSTMLGLANVPSILLAEKIIEVVPEGLKK
HCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFYSDSGSSAVEIAIKMAFQYWQHKGKPKKQRFVTLKEAYHGDTIGAVSVGAIDLFHQVY
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SSLLFEAIKMPYPYTYRSPYGNDKAEIVKKHLEEMEELLKDKHEEIAAIIVEPLMQGAGG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MITMPKGYLRGLRNLCTKYNVLFITDEVATGFGRTGKMFACEHENVTPDILTAGKGLTGG
EEECCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCCCC
YLPVAITVTTDEIYNAFLGSYEEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNLIEEVA
CEEEEEEEEHHHHHHHHHCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKTEYVATQLEELFACKHVGDIRQCGLMVGIELVKNKETKEAFEWTERVGVQVCKRSREL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GMILRPLGNTIVFMPPLASTIDEIDEMLRILYKAISDITEGE
HHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]