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The map label for this gene is yhcA [H]

Identifier: 49480898

GI number: 49480898

Start: 3062199

End: 3063677

Strand: Reverse

Name: yhcA [H]

Synonym: BT9727_2982

Alternate gene names: 49480898

Gene position: 3063677-3062199 (Counterclockwise)

Preceding gene: 49478075

Following gene: 49480099

Centisome position: 58.49

GC content: 38.2

Gene sequence:

>1479_bases
ATGAGTGAAACGTTACATAAAAGAAAACCACCATATGTAATGCTTGTAATCCTTTTTATTGGGGCATTTGTTTCATTTCT
AAATAACTCGCTTTTAAATGTGGCCTTGCCATCAATAATGAAGGATTTAGAAATTCAAGATTATTCAACGATCCAGTGGC
TTTCTACAGGATATATGCTTGTAAGTGGTATATTAATTCCGGCATCAGCATTTTTAATAACCCGCTTCTCCAATAGGAGT
TTATTTATTACATCTATGGTGATTTTTACTCTTGGTACGGCTTTAGCGGCTGTAGCACCGAACTTCGGTTTATTACTTAC
TGGTCGGATGGTTCAAGCAGCAGGTTCTTCAGTGATGGGACCTTTGTTAATGAATATTATGCTAGTAAGCTTTCCGAGAG
AAAAAAGGGGAACAGCAATGGGGATTTTTGGACTAGTTATGATTACAGCCCCAGCTATTGGACCGACATTATCAGGTTAT
ATTGTAGAGTATTATGACTGGCGTTTGCTTTTTGAAATGATTTTACCGTTAGCAATCATTAGCTTACTGTTAGGGATTTG
GAAATCAGAGAATGTCATGAGACAAAACAAAAATGCAAAACTTGATTATCTTTCATTACTATTATCTTCTATCGGTTTTG
GCGGTTTATTATATGGGTTCAGTTCTGCAAGCTCCGATGGTTGGACGAATAAAGTTGTCGTAACAACCTTAATCATAGGC
GCTATTGCCCTTATTGCTTTCATTATCCGTCAATTAAAAATGAATGAACCGTTATTGGATTTACGTGTTTATAAGTATCC
AATGTTTGCACTTGCGTCTGTAATTGCGATTGTCAACGCAGTGGCCATGTTTTCGGGTATGATTTTAACACCAGCTTATG
TACAAAACGTTCGCGGTATTTCACCGCTGAGCTCTGGGCTGATGATGCTTCCAGGTGCCGTAATAATGGGGATTATGTCA
CCAATTACAGGTAAACTATTTGATAAATATGGCCCTCGTATTCTTGGTATAGTAGGACTTTCCATTACAGCCGTTTCAAC
TTATATGCTAGCAAACCTACAACTCGACTCTAGTCACACACATATTATTCTTATTTACACACTGCGGATGTTTGGGATGG
CGATGGTGATGATGCCGCTTATGACAAATGGCCTTAATCAATTGCCAACTCGCTTAAATCCGCACGGTACAGCTGTTAAT
AATACAGCTCAACAAGTATCCGGTTCAATTGGTACTGCTATTCTTGTTACCATTATGAATTCTGTAACAAAAACAAAAGC
TGAAAGTTTAATGGCTCATGTAGATCCAACAAACTTCACAGAAGCTACGAAGATAATGTTAACGCAGAAGGCTTTATTAT
CCGGAATTCAGCATTCATTCTATGTAGCACTTGGCATGAATGTCGTTGCGCTACTATTAGTTTTTTTCGTTAAACGTGTA
GATACAAGCGTGGAAGCTGTCGAGAGGTTAAATCGGTAG

Upstream 100 bases:

>100_bases
AGAGTTTGGCAAAATACTGCAAGGGTAATAAATTCTCGTGCAAATATGCAAACGAGTTAAATTAATCAATGGAATTGATA
ATGAAGGGGTAAATCAATCT

Downstream 100 bases:

>100_bases
AGCAAAATAAAACTCGAGATGATGATTTCTACTAAAAGGGGAAAAGAGATGAAAGAAAAAGTGAAATAATTACACAGAAT
GAAAAAGGATTTGATATGAA

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLEIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMVIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLIIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSVEAVERLNR

Sequences:

>Translated_492_residues
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLEIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRS
LFITSMVIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGY
IVEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLIIG
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMS
PITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVN
NTAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
DTSVEAVERLNR
>Mature_491_residues
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLEIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSL
FITSMVIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYI
VEYYDWRLLFEMILPLAIISLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLIIGA
IALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSP
ITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNN
TAQQVSGSIGTAILVTIMNSVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRVD
TSVEAVERLNR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=451, Percent_Identity=26.6075388026608, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=24.0246406570842, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=26.0975609756098, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=27.0935960591133, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=26.1728395061728, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322958, Length=529, Percent_Identity=21.3610586011342, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=328, Percent_Identity=23.1707317073171, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=239, Percent_Identity=23.4309623430962, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53576; Mature: 53445

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLEIQDYSTIQWLSTGYML
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMVIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLIIG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTSVEAVERLNR
CHHHHHHHHHCC
>Mature Secondary Structure 
SETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLEIQDYSTIQWLSTGYML
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSGILIPASAFLITRFSNRSLFITSMVIFTLGTALAAVAPNFGLLLTGRMVQAAGSSVMG
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCHHHH
PLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAII
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLLGIWKSENVMRQNKNAKLDYLSLLLSSIGFGGLLYGFSSASSDGWTNKVVVTTLIIG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
AIALIAFIIRQLKMNEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHCCCC
SPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLANLQLDSSHT
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHEEEECCCCC
HIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMN
EEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
SVTKTKAESLMAHVDPTNFTEATKIMLTQKALLSGIQHSFYVALGMNVVALLLVFFVKRV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTSVEAVERLNR
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]