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The map label for this gene is amaA

Identifier: 49480897

GI number: 49480897

Start: 3051392

End: 3052216

Strand: Reverse

Name: amaA

Synonym: BT9727_2973

Alternate gene names: 49480897

Gene position: 3052216-3051392 (Counterclockwise)

Preceding gene: 49478072

Following gene: 49480094

Centisome position: 58.27

GC content: 42.06

Gene sequence:

>825_bases
GTGTTCGGAACACCAGCTGAAGAAGGAGGACCGAACGGTAGCGCAAAATCGAGTTATGTAAAGGCAGGTTTATTTAAAAA
TATTGATGCGGCGCTTATGATTCATCCGAGCGGAAAGACAGCAACAACAAGTCCTTCACTAGCAGTCGATCCACTTGATT
TTCATTTTTATGGAAAAACAGCTCATGCAGCAGCGTCACCTGAAGAAGGAATTAACGCATTAGATGCGGTGATTCAGCTG
TACAACAGCATTAACGCACTTCGCCAACAACTTCCGTCAGACGTGAAAATTCATGGCGTTATTACAGAAGGCGGAAAAGC
ACCTAACATTATTCCTGACTACGCCGCAGCAAGATTCTTCATCCGTGCAGCAACACGAAAAAGATGTGCGGAAGTAACAG
AAAAAGTAAAAAACATTGCACAGGGAGCAGCGTTAGCAACAGGAACAAAAGTAAAAATCCATCAATTCCAAAATGAAATC
GATGAACTACTCGTAACAAAAACATTTAATGAAGTCGTAGCCGAAGAACTAGAATTACAAGGTGAAGACGTAAATCGTAA
AGAAAGATTTGGTATTGGTTCAACCGATGCAGGAAACGTTAGCCAAGTCGTACCGACAATCCACCCGTACATTAAAATCG
GCCCAGATGATTTAATTGCACATACGAATGAATTTAGAGAAGCAGCACGTTCAGAATTAGGAGACAAAGCTCTAATTACA
TCAGCAAAAGCACTAGCAAATACCGCGTATCGATTAATTACGGAAGAAGGGTTGTTAGAGAAGGTGAAGGAGGAGTTTAG
AGAGGCGCAGAGAAATCAGGGGTAG

Upstream 100 bases:

>100_bases
ACTTGGCCATGCGTGTGGTCACAATTTAATTGGCACCATTAGCGTTGCAGCAGCGATCGCATTATCAGAAACACTCTAAG
AAATTGGCGGAGAAGTTGTC

Downstream 100 bases:

>100_bases
GTGGTTAAAGGGAGTGGATAATCCACTCCTTTTTCTTTTGTGGAAAGCTGGTGCCTATCACTTTATTAGAACGACAATTT
CTCCTCAAACTCACTCTTTA

Product: N-acyl-L-amino acid amidohydrolase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MFGTPAEEGGPNGSAKSSYVKAGLFKNIDAALMIHPSGKTATTSPSLAVDPLDFHFYGKTAHAAASPEEGINALDAVIQL
YNSINALRQQLPSDVKIHGVITEGGKAPNIIPDYAAARFFIRAATRKRCAEVTEKVKNIAQGAALATGTKVKIHQFQNEI
DELLVTKTFNEVVAEELELQGEDVNRKERFGIGSTDAGNVSQVVPTIHPYIKIGPDDLIAHTNEFREAARSELGDKALIT
SAKALANTAYRLITEEGLLEKVKEEFREAQRNQG

Sequences:

>Translated_274_residues
MFGTPAEEGGPNGSAKSSYVKAGLFKNIDAALMIHPSGKTATTSPSLAVDPLDFHFYGKTAHAAASPEEGINALDAVIQL
YNSINALRQQLPSDVKIHGVITEGGKAPNIIPDYAAARFFIRAATRKRCAEVTEKVKNIAQGAALATGTKVKIHQFQNEI
DELLVTKTFNEVVAEELELQGEDVNRKERFGIGSTDAGNVSQVVPTIHPYIKIGPDDLIAHTNEFREAARSELGDKALIT
SAKALANTAYRLITEEGLLEKVKEEFREAQRNQG
>Mature_274_residues
MFGTPAEEGGPNGSAKSSYVKAGLFKNIDAALMIHPSGKTATTSPSLAVDPLDFHFYGKTAHAAASPEEGINALDAVIQL
YNSINALRQQLPSDVKIHGVITEGGKAPNIIPDYAAARFFIRAATRKRCAEVTEKVKNIAQGAALATGTKVKIHQFQNEI
DELLVTKTFNEVVAEELELQGEDVNRKERFGIGSTDAGNVSQVVPTIHPYIKIGPDDLIAHTNEFREAARSELGDKALIT
SAKALANTAYRLITEEGLLEKVKEEFREAQRNQG

Specific function: Required but not essential for aminobenzoyl-glutamate utilization. May participate in hydrolysis of aminobenzoyl- glutamate to aminobenzoate, either alone or in combination with AbgA [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI58082085, Length=278, Percent_Identity=37.7697841726619, Blast_Score=181, Evalue=5e-46,
Organism=Escherichia coli, GI1787598, Length=179, Percent_Identity=33.5195530726257, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017145
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 29597; Mature: 29597

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTPAEEGGPNGSAKSSYVKAGLFKNIDAALMIHPSGKTATTSPSLAVDPLDFHFYGKT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEECCCEEEEECCC
AHAAASPEEGINALDAVIQLYNSINALRQQLPSDVKIHGVITEGGKAPNIIPDYAAARFF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
IRAATRKRCAEVTEKVKNIAQGAALATGTKVKIHQFQNEIDELLVTKTFNEVVAEELELQ
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDVNRKERFGIGSTDAGNVSQVVPTIHPYIKIGPDDLIAHTNEFREAARSELGDKALIT
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
SAKALANTAYRLITEEGLLEKVKEEFREAQRNQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFGTPAEEGGPNGSAKSSYVKAGLFKNIDAALMIHPSGKTATTSPSLAVDPLDFHFYGKT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEECCCEEEEECCC
AHAAASPEEGINALDAVIQLYNSINALRQQLPSDVKIHGVITEGGKAPNIIPDYAAARFF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
IRAATRKRCAEVTEKVKNIAQGAALATGTKVKIHQFQNEIDELLVTKTFNEVVAEELELQ
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDVNRKERFGIGSTDAGNVSQVVPTIHPYIKIGPDDLIAHTNEFREAARSELGDKALIT
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
SAKALANTAYRLITEEGLLEKVKEEFREAQRNQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9829935 [H]