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The map label for this gene is yfkF [H]

Identifier: 49480602

GI number: 49480602

Start: 3154033

End: 3155229

Strand: Reverse

Name: yfkF [H]

Synonym: BT9727_3083

Alternate gene names: 49480602

Gene position: 3155229-3154033 (Counterclockwise)

Preceding gene: 49480117

Following gene: 49479502

Centisome position: 60.24

GC content: 38.35

Gene sequence:

>1197_bases
ATGTCAATGCGTTTTACTTTTTGGATTATGGTTGGGATTGTAGCAATCTCGGGTTTGTCACAAGGGATGCTTTTACCGGC
CATTGCAATGATTTTTGAACAAGAAGGGGTAAGTTCAAGTATTAACGGTATTCATGCGACAGCGCTATACATTGGGATAT
TAGTTATTTCCCCGTTTCTTGAAAAACCGATGCAAAAGTTTGGAATGAAGCCAATTATTGTGATTGGTGGGTTTCTCGTT
ATCATTTCATTATTCTTTTTTACACAAACCTTTTCATTCTGGGTATGGTTTATCCTTAGATTTCTAGTTGGAGTCGGAGA
TCATATGCTTCATGTCGGAACACAAACATGGATTACGACAACATCAGACCCGAGTAAAATAGGGAGACAAGTATCGATAT
ACGGTGTATTCTTCGGAATTGGTTTTGCCGTCGGTCCGTATTTAGCAAGCACTGTTCAATACGGTCTTGCAACGCCATTT
ATTATATCTACTATACTTTGTTTAATAGGTTGGCTGTTACTATTACCAACAAAAAATGCATTCCCAGCGCAAGATGAAAG
AGAAGTGAAGCGTGAATCATCATTTTCTCGTTATAAACAAGTTGTTGGATTAGGATGGATTGCATTACTGGGACCACTTG
CATATGGCGTACTGGAAGCGATGTTAAATAGTAACTTACCAGTATACGCGCTTCGAAAAGGATGGTCCGTCTCAGAAGTA
TCCTTCTTATTGCCAGCGTTTGCAGTTGGGGGTATTATTACACAAATTCCGCTCGGTATATTAAGTGACAAATACGGAAG
GGATCGTATTTTAACGTGGACGTTCTGCATAAGTACGGGCATTTTCTTGCTTGCAGCCGTATTTGATCAATATTACTGGA
TCGTCTTTGCCTGTATGCTATTAGCGGGTATGGTCATTGGATCGTGCTTCTCTTTAGGACTTGGATTTATGACAGATTTA
TTACCGAGGCACTTATTGCCAGCAGGTAATATATTATCTGGAATCGCCTTTAGTTTAGGAAGTATACTGGGACCTGTATT
AGGAGGCGTATTTATAGAAAAAATACAGTATACAAGCTTTTTTATTGCGGTTATGATTATAATAGGAACTCTCGCAATAT
TATATATGATCTACATGAAGAATCAATTTGCATCAAGAAAAATAGAAAGTAGGTTAGGGCATGACAAAACAACCTAA

Upstream 100 bases:

>100_bases
TTGTGGAAAAATGAAAATTATTTCATCCACTAGTTCTTTCTTATTACATAGCAAACAATATATAATGAGTGTGGTGGATT
ACAAAAGGGAGAGATTAGGG

Downstream 100 bases:

>100_bases
TAAAAAGAAATTTGAAGTACTCGAAAATGAAACAATTACAGACTGCTTAGCACGTATGGAACAAGAAGGCTATGCACCGT
CTCGCCGTATGGAAGAGCCA

Product: multidrug-efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQKFGMKPIIVIGGFLV
IISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPF
IISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSEV
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDL
LPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFIAVMIIIGTLAILYMIYMKNQFASRKIESRLGHDKTT

Sequences:

>Translated_398_residues
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQKFGMKPIIVIGGFLV
IISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPF
IISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSEV
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDL
LPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFIAVMIIIGTLAILYMIYMKNQFASRKIESRLGHDKTT
>Mature_397_residues
SMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQKFGMKPIIVIGGFLVI
ISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFI
ISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSEVS
FLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDLL
PRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFIAVMIIIGTLAILYMIYMKNQFASRKIESRLGHDKTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787126, Length=351, Percent_Identity=22.5071225071225, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43814; Mature: 43683

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHH
EKPMQKFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
TSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNA
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FPAQDEREVKRESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQYYWIVFACML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVMIIIGTLAILYMIYMKNQFASRKIESRLGHDKTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHH
EKPMQKFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
TSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNA
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FPAQDEREVKRESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQYYWIVFACML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVMIIIGTLAILYMIYMKNQFASRKIESRLGHDKTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]