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The map label for this gene is opuD

Identifier: 49480460

GI number: 49480460

Start: 5191565

End: 5193133

Strand: Reverse

Name: opuD

Synonym: BT9727_5125

Alternate gene names: 49480460

Gene position: 5193133-5191565 (Counterclockwise)

Preceding gene: 49481233

Following gene: 49480458

Centisome position: 99.15

GC content: 38.11

Gene sequence:

>1569_bases
ATGAGGATGAAGAGTCGGAAAACGGATTGGCCTGTATTTCTTATTAGTGGTGGCTCACTTTTATTATTTGTAATTGCGGT
TTTTTTTAATAAAAGTTACGTAGAGGGAGTCATTAATAGCAGTTTTGCAGCTTCAATTAAATATTTTGGTGCCTTTTGGC
AGGTTTTATTAATCGGTACATTTATTGTTGCAATGTGTATGGCGTTCTCAAAGTATGGGAGAGTTAAACTTGGGGGATTA
GAAAAACCTGAGATTAGTACGACGAAATGGCTCGCTATTATAATGTCTACATTACTGGCCGGAGGTGGTGTTTTCTGGGC
AGCGGCAGAGCCGATGTACCATTTAATGACGGTGCCACCAATACATGAAGGTATAACTGCTGGAACGAAAGAAGCAGTAA
TGCCTGCTTTAGCACAAAGTTATATGCACTGGGGTTTCTTGGCTTGGACGATTTTAGGGACAATTAGTGCGGTAGTTATG
ATGTATGGGCATTATCATAAAGGGATGCCGTTAAAACCTCGAACGCTTTTATATCCTATTTTTGGAGAGAAATTGCGAAA
GAGTTGGCTTGGAACACTAATTGATGCATTTGCCATTATTGCAGTAGCTGCAGGGACGATTGGTCCAATCGGATTTTTAG
GTCTACAAGCAAGTTATGGCCTACAAGCATTGTTTAACATCCCGGATGTGTTTACAACTCAATTAGCCATTATTGTTTGT
GTAGTAGCTGTTTCTACTATATCTGCGGTGACTGGTATTGATAAAGGGATTCAAATTATAAGTAATTTAAATGTTAGATT
GGCAATTTTATTAATGGTATTCGTATTACTATTTGGACCAGGTGGATTTATTATTGATTCGTTTGTATCTTCGTTTGGAT
TTTATATAAATGAATTTATTCCAATGAGCACATACCGCGGTGATACAGGTTGGTTAGGATCGTGGACAATCTTTTTCTGG
GGATGGTTTATTGGATATGGACCGATGATGGCAATTTTAGTGAGCCGCATTTCAAGAGGAAGAACAATTCGAGAAATCAT
CGTTGCAATTGGAATTATTGCACCTATTATTACAACGTTTTGGTTTACTATACTAGGGGGATCAGGTGTGTTTTATGAGT
TAATGAATCCTGGTTCTATATCGACCGCATTAAGTGCATCTGGTATGCCGGCAGCTATGATTGCAATTACAGAGCAACTG
CCACTTTCTCATATTATTGGACCTGCATTTCTTTTATTAACAATTTTATTTGTAGTGACAACAGGAGATTCAATGGCGTA
TTCGATTTCAATGGCAGTAACTGGAGATGGCGATCCTAGAATTAGTTTGCGAGTTTTTTGGTCGCTTATTATGGGAACCG
TTGCAGCGATTCTTTTATATATGGGTGAGGGTAGTATTAATGCATTGCAATCATTCATCGTAGTAACAGCTGTCCCAGTA
TCAATTTTGTTATTCCCAATGCTATGGCTAGCCCCTAAAGTCGCAGGGGAATTAGCACTAAAACAAGGGATTGTAAAAGA
AGAAGATAAAACGGCTTTCTTATTTCAAAAGGCTAGTAAATCAAAATAA

Upstream 100 bases:

>100_bases
AGATGAATTATTATACTTATATAATCATTGTGTTTTGGGTGTAAACAATAAAACATAATAAAGTTGTTTTTTTAGATTGC
TGAGGAAGGAGGAAGTGTGA

Downstream 100 bases:

>100_bases
TAAATAAACAAATAAAAGAGGAAGCGTCTTAAATGAAATGCTTCCTCTTTACTATTTATTCAAATACTTTCCATGAAGCA
GGTAGTACTCGAACAATCAT

Product: BCCT family osmoprotectant transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MRMKSRKTDWPVFLISGGSLLLFVIAVFFNKSYVEGVINSSFAASIKYFGAFWQVLLIGTFIVAMCMAFSKYGRVKLGGL
EKPEISTTKWLAIIMSTLLAGGGVFWAAAEPMYHLMTVPPIHEGITAGTKEAVMPALAQSYMHWGFLAWTILGTISAVVM
MYGHYHKGMPLKPRTLLYPIFGEKLRKSWLGTLIDAFAIIAVAAGTIGPIGFLGLQASYGLQALFNIPDVFTTQLAIIVC
VVAVSTISAVTGIDKGIQIISNLNVRLAILLMVFVLLFGPGGFIIDSFVSSFGFYINEFIPMSTYRGDTGWLGSWTIFFW
GWFIGYGPMMAILVSRISRGRTIREIIVAIGIIAPIITTFWFTILGGSGVFYELMNPGSISTALSASGMPAAMIAITEQL
PLSHIIGPAFLLLTILFVVTTGDSMAYSISMAVTGDGDPRISLRVFWSLIMGTVAAILLYMGEGSINALQSFIVVTAVPV
SILLFPMLWLAPKVAGELALKQGIVKEEDKTAFLFQKASKSK

Sequences:

>Translated_522_residues
MRMKSRKTDWPVFLISGGSLLLFVIAVFFNKSYVEGVINSSFAASIKYFGAFWQVLLIGTFIVAMCMAFSKYGRVKLGGL
EKPEISTTKWLAIIMSTLLAGGGVFWAAAEPMYHLMTVPPIHEGITAGTKEAVMPALAQSYMHWGFLAWTILGTISAVVM
MYGHYHKGMPLKPRTLLYPIFGEKLRKSWLGTLIDAFAIIAVAAGTIGPIGFLGLQASYGLQALFNIPDVFTTQLAIIVC
VVAVSTISAVTGIDKGIQIISNLNVRLAILLMVFVLLFGPGGFIIDSFVSSFGFYINEFIPMSTYRGDTGWLGSWTIFFW
GWFIGYGPMMAILVSRISRGRTIREIIVAIGIIAPIITTFWFTILGGSGVFYELMNPGSISTALSASGMPAAMIAITEQL
PLSHIIGPAFLLLTILFVVTTGDSMAYSISMAVTGDGDPRISLRVFWSLIMGTVAAILLYMGEGSINALQSFIVVTAVPV
SILLFPMLWLAPKVAGELALKQGIVKEEDKTAFLFQKASKSK
>Mature_522_residues
MRMKSRKTDWPVFLISGGSLLLFVIAVFFNKSYVEGVINSSFAASIKYFGAFWQVLLIGTFIVAMCMAFSKYGRVKLGGL
EKPEISTTKWLAIIMSTLLAGGGVFWAAAEPMYHLMTVPPIHEGITAGTKEAVMPALAQSYMHWGFLAWTILGTISAVVM
MYGHYHKGMPLKPRTLLYPIFGEKLRKSWLGTLIDAFAIIAVAAGTIGPIGFLGLQASYGLQALFNIPDVFTTQLAIIVC
VVAVSTISAVTGIDKGIQIISNLNVRLAILLMVFVLLFGPGGFIIDSFVSSFGFYINEFIPMSTYRGDTGWLGSWTIFFW
GWFIGYGPMMAILVSRISRGRTIREIIVAIGIIAPIITTFWFTILGGSGVFYELMNPGSISTALSASGMPAAMIAITEQL
PLSHIIGPAFLLLTILFVVTTGDSMAYSISMAVTGDGDPRISLRVFWSLIMGTVAAILLYMGEGSINALQSFIVVTAVPV
SILLFPMLWLAPKVAGELALKQGIVKEEDKTAFLFQKASKSK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=493, Percent_Identity=31.4401622718053, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786224, Length=454, Percent_Identity=27.0925110132159, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788102, Length=468, Percent_Identity=28.4188034188034, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56652; Mature: 56652

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKSRKTDWPVFLISGGSLLLFVIAVFFNKSYVEGVINSSFAASIKYFGAFWQVLLIGT
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FIVAMCMAFSKYGRVKLGGLEKPEISTTKWLAIIMSTLLAGGGVFWAAAEPMYHLMTVPP
HHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHEECCC
IHEGITAGTKEAVMPALAQSYMHWGFLAWTILGTISAVVMMYGHYHKGMPLKPRTLLYPI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEHH
FGEKLRKSWLGTLIDAFAIIAVAAGTIGPIGFLGLQASYGLQALFNIPDVFTTQLAIIVC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHH
VVAVSTISAVTGIDKGIQIISNLNVRLAILLMVFVLLFGPGGFIIDSFVSSFGFYINEFI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
PMSTYRGDTGWLGSWTIFFWGWFIGYGPMMAILVSRISRGRTIREIIVAIGIIAPIITTF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
WFTILGGSGVFYELMNPGSISTALSASGMPAAMIAITEQLPLSHIIGPAFLLLTILFVVT
HHHHHCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TGDSMAYSISMAVTGDGDPRISLRVFWSLIMGTVAAILLYMGEGSINALQSFIVVTAVPV
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SILLFPMLWLAPKVAGELALKQGIVKEEDKTAFLFQKASKSK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRMKSRKTDWPVFLISGGSLLLFVIAVFFNKSYVEGVINSSFAASIKYFGAFWQVLLIGT
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FIVAMCMAFSKYGRVKLGGLEKPEISTTKWLAIIMSTLLAGGGVFWAAAEPMYHLMTVPP
HHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHEECCC
IHEGITAGTKEAVMPALAQSYMHWGFLAWTILGTISAVVMMYGHYHKGMPLKPRTLLYPI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEHH
FGEKLRKSWLGTLIDAFAIIAVAAGTIGPIGFLGLQASYGLQALFNIPDVFTTQLAIIVC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHH
VVAVSTISAVTGIDKGIQIISNLNVRLAILLMVFVLLFGPGGFIIDSFVSSFGFYINEFI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
PMSTYRGDTGWLGSWTIFFWGWFIGYGPMMAILVSRISRGRTIREIIVAIGIIAPIITTF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
WFTILGGSGVFYELMNPGSISTALSASGMPAAMIAITEQLPLSHIIGPAFLLLTILFVVT
HHHHHCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TGDSMAYSISMAVTGDGDPRISLRVFWSLIMGTVAAILLYMGEGSINALQSFIVVTAVPV
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SILLFPMLWLAPKVAGELALKQGIVKEEDKTAFLFQKASKSK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]