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The map label for this gene is rpoE

Identifier: 49480420

GI number: 49480420

Start: 5073520

End: 5074050

Strand: Reverse

Name: rpoE

Synonym: BT9727_5023

Alternate gene names: 49480420

Gene position: 5074050-5073520 (Counterclockwise)

Preceding gene: 49480421

Following gene: 49480419

Centisome position: 96.88

GC content: 38.61

Gene sequence:

>531_bases
GTGGATTTTAAGCAATATTCACCAGAAGAGCTAAAAGAATGTTCAATGATTGAAGTTGTACATAGCGTTTTAGGGGATAA
AAGACAAGCAACGACATTCAATGAGTTGGTTCAAGAAATCGCTCAAGTGCTGGGACTATCTCAAGAGCAAGTTAATGCGA
AAATCGCACAATTTTATACAGACTTAAACATCGATGGACGTTTCATTAATTTAGGAGAAAATCGTTGGGGATTACGTAGC
TGGTACCCATATGAGCAAATTGATGAAGAAATCTTGCCTCAACCAAAACCGAAGAAGAAACGTAAAGTTGAAGACGACGG
TTTTGACGACTACATTGAAGAAGATGAAGACTTCGACGATGCAGACGTAACTGAAGACGAAGATGATGATGTAGAAGATT
TGGACAAGGTTCTTGAAGATGAGGACGGAGATGACGATGATCTTGATGATTTAGATGAAGATGATGATGACTTCGCTGAA
GAAGAACTTGAGTATGATGAAACTGAAGAAGAAGAAGAGGAAGAACTGTAG

Upstream 100 bases:

>100_bases
AGCATATTTGTGCGACTTTTGTTTATAATGAATAGTATGGATTTTTTTAAGCAGGCCGTTATATATAAGGTTATATTTGA
TGAAAGGAAGTGCCGCATAA

Downstream 100 bases:

>100_bases
TTCTTGACTTTTCATTATCGTCCATGTAGAATCATTTTTGGGCTCTTTAAAAAAGGACGATAATACTTTTAAAGTGTAAA
TATAAAAGAAAATTGCTCCC

Product: DNA-directed RNA polymerase subunit delta

Products: diphosphate; RNAn+1

Alternate protein names: RNAP delta factor [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDFKQYSPEELKECSMIEVVHSVLGDKRQATTFNELVQEIAQVLGLSQEQVNAKIAQFYTDLNIDGRFINLGENRWGLRS
WYPYEQIDEEILPQPKPKKKRKVEDDGFDDYIEEDEDFDDADVTEDEDDDVEDLDKVLEDEDGDDDDLDDLDEDDDDFAE
EELEYDETEEEEEEEL

Sequences:

>Translated_176_residues
MDFKQYSPEELKECSMIEVVHSVLGDKRQATTFNELVQEIAQVLGLSQEQVNAKIAQFYTDLNIDGRFINLGENRWGLRS
WYPYEQIDEEILPQPKPKKKRKVEDDGFDDYIEEDEDFDDADVTEDEDDDVEDLDKVLEDEDGDDDDLDDLDEDDDDFAE
EELEYDETEEEEEEEL
>Mature_176_residues
MDFKQYSPEELKECSMIEVVHSVLGDKRQATTFNELVQEIAQVLGLSQEQVNAKIAQFYTDLNIDGRFINLGENRWGLRS
WYPYEQIDEEILPQPKPKKKRKVEDDGFDDYIEEDEDFDDADVTEDEDDDVEDLDKVLEDEDGDDDDLDDLDEDDDDFAE
EELEYDETEEEEEEEL

Specific function: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis

COG id: COG3343

COG function: function code K; DNA-directed RNA polymerase, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rpoE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007759 [H]

Pfam domain/function: PF05066 RNA_pol_delta [H]

EC number: 2.7.7.6

Molecular weight: Translated: 20709; Mature: 20709

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQYSPEELKECSMIEVVHSVLGDKRQATTFNELVQEIAQVLGLSQEQVNAKIAQFYT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLNIDGRFINLGENRWGLRSWYPYEQIDEEILPQPKPKKKRKVEDDGFDDYIEEDEDFDD
CCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCHHHHCCCCCCCC
ADVTEDEDDDVEDLDKVLEDEDGDDDDLDDLDEDDDDFAEEELEYDETEEEEEEEL
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure
MDFKQYSPEELKECSMIEVVHSVLGDKRQATTFNELVQEIAQVLGLSQEQVNAKIAQFYT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DLNIDGRFINLGENRWGLRSWYPYEQIDEEILPQPKPKKKRKVEDDGFDDYIEEDEDFDD
CCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCHHHHCCCCCCCC
ADVTEDEDDDVEDLDKVLEDEDGDDDDLDDLDEDDDDFAEEELEYDETEEEEEEEL
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA