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The map label for this gene is rho

Identifier: 49480416

GI number: 49480416

Start: 5065091

End: 5066362

Strand: Reverse

Name: rho

Synonym: BT9727_5016

Alternate gene names: 49480416

Gene position: 5066362-5065091 (Counterclockwise)

Preceding gene: 49481201

Following gene: 49481754

Centisome position: 96.73

GC content: 37.89

Gene sequence:

>1272_bases
ATGAATTTGTCAATTGCAGCATTAGAAAACATGAAATTAAAAGAGTTATACGAGCTTGCGAAAGAATTTAAGATTTCGTA
TTATAGCAAATTAACGAAAAAAGAGTTAATCTTCGCCATTTTAAAAGCTCGAGCAGAAAAAGAAGGTTTCTTCTTCATGG
AAGGCGTATTAGAAATTATTCAATCAGAAGGATTTGGATTCCTACGTCCTATCAACTACTCTCCAAGCTCAGAAGATATT
TATATCTCAGCTTCGCAAATTCGTCGTTTCGATTTACGTAATGGAGATAAAGTTTCTGGTAAAGTACGACCTCCGAAAGA
AAATGAACGCTACTTTGGATTATTACAAGTTGAAGCTGTAAATGGAGATGATCCAGAGTCAGCAAAAGAGCGTGTGCATT
TCCCTGCATTAACACCATTATACCCAGATCGCCAAATGAAATTGGAAACGGAACCGAAAAAGTTACCGACACGCATCATG
GATTTAATTGCACCAGTTGGATTTGGACAACGTGGTTTAATTGTCGCGCCTCCAAAGGCTGGTAAAACAAGTCTATTAAA
AGAAATCGCGCACAGTGTTACAACAAATCATCCGGAAGCTGAATTAATTGTACTTTTAATTGATGAGCGTCCAGAGGAAG
TAACAGACATTGAACGTTCTGTTAAAGGAGATGTTGTAAGCTCTACTTTTGATGAAGTACCAGAAAATCATATTAAAGTA
GCGGAACTTGTGTTAGAACGTGCAATGCGTCTTGTAGAGCACAAAAAAGATGTTATCATTTTAATGGATAGTATTACCCG
TTTAGCGCGAGCTTACAACCTTGTTATTCCGCCAAGTGGTAGAACATTATCAGGTGGTATCGACCCAGCTGCCTTCCATA
GACCGAAGCGCTTCTTTGGAGCTGCACGTAATATTGAAGAAGGCGGTAGCTTAACTATTTTAGCAACAGCGCTTGTTGAT
ACAGGATCTCGTATGGACGATGTAATTTACGAAGAATTTAAAGGAACTGGAAATATGGAACTTCACTTAGATCGTTCATT
AGCTGAGCGTCGTATCTTCCCGGCAATTGATATTCGCCGTTCTGGTACACGTAAAGAGGATCTATTAATTCCGAAAGAAC
ATTTAGACAAGCTGTGGGGTATTCGTAAAACAATGCGTGATACACCAGACTTTGTTGAAAGTTTCTTACGTAAACTTCGT
CAAACAAAGACAAATGAAGAATTTTTACAAAACATTGTTGCAGATTCGAAAAGATATGTAACAACTAAGTAA

Upstream 100 bases:

>100_bases
CCTACTTGAACTTATTGCCTTTCATTATTAATTATTTGCCTTCCGTATTTTTTCCCTTTACCCATGTGAAAAGAGAATAA
CGTTAAAGTGTGGTGTCTGA

Downstream 100 bases:

>100_bases
AGGGAAAAGATTGGGACTCGCTTACTTTGTGGTAAGCGAGTTTTTTATGTTTTTTAGGTGATGTTAAAGAGAGAGGAATG
GAAGAATCTCCGGTCATTCC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNLSIAALENMKLKELYELAKEFKISYYSKLTKKELIFAILKARAEKEGFFFMEGVLEIIQSEGFGFLRPINYSPSSEDI
YISASQIRRFDLRNGDKVSGKVRPPKENERYFGLLQVEAVNGDDPESAKERVHFPALTPLYPDRQMKLETEPKKLPTRIM
DLIAPVGFGQRGLIVAPPKAGKTSLLKEIAHSVTTNHPEAELIVLLIDERPEEVTDIERSVKGDVVSSTFDEVPENHIKV
AELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALVD
TGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEDLLIPKEHLDKLWGIRKTMRDTPDFVESFLRKLR
QTKTNEEFLQNIVADSKRYVTTK

Sequences:

>Translated_423_residues
MNLSIAALENMKLKELYELAKEFKISYYSKLTKKELIFAILKARAEKEGFFFMEGVLEIIQSEGFGFLRPINYSPSSEDI
YISASQIRRFDLRNGDKVSGKVRPPKENERYFGLLQVEAVNGDDPESAKERVHFPALTPLYPDRQMKLETEPKKLPTRIM
DLIAPVGFGQRGLIVAPPKAGKTSLLKEIAHSVTTNHPEAELIVLLIDERPEEVTDIERSVKGDVVSSTFDEVPENHIKV
AELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALVD
TGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEDLLIPKEHLDKLWGIRKTMRDTPDFVESFLRKLR
QTKTNEEFLQNIVADSKRYVTTK
>Mature_423_residues
MNLSIAALENMKLKELYELAKEFKISYYSKLTKKELIFAILKARAEKEGFFFMEGVLEIIQSEGFGFLRPINYSPSSEDI
YISASQIRRFDLRNGDKVSGKVRPPKENERYFGLLQVEAVNGDDPESAKERVHFPALTPLYPDRQMKLETEPKKLPTRIM
DLIAPVGFGQRGLIVAPPKAGKTSLLKEIAHSVTTNHPEAELIVLLIDERPEEVTDIERSVKGDVVSSTFDEVPENHIKV
AELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALVD
TGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEDLLIPKEHLDKLWGIRKTMRDTPDFVESFLRKLR
QTKTNEEFLQNIVADSKRYVTTK

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=415, Percent_Identity=57.5903614457831, Blast_Score=486, Evalue=1e-139,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48082; Mature: 48082

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSIAALENMKLKELYELAKEFKISYYSKLTKKELIFAILKARAEKEGFFFMEGVLEII
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QSEGFGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYFGLLQVEAV
HHCCCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
NGDDPESAKERVHFPALTPLYPDRQMKLETEPKKLPTRIMDLIAPVGFGQRGLIVAPPKA
CCCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GKTSLLKEIAHSVTTNHPEAELIVLLIDERPEEVTDIERSVKGDVVSSTFDEVPENHIKV
CHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFG
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHCCHHHHH
AARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRR
HHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCEECCC
SGTRKEDLLIPKEHLDKLWGIRKTMRDTPDFVESFLRKLRQTKTNEEFLQNIVADSKRYV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
TTK
CCC
>Mature Secondary Structure
MNLSIAALENMKLKELYELAKEFKISYYSKLTKKELIFAILKARAEKEGFFFMEGVLEII
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QSEGFGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYFGLLQVEAV
HHCCCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
NGDDPESAKERVHFPALTPLYPDRQMKLETEPKKLPTRIMDLIAPVGFGQRGLIVAPPKA
CCCCCHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GKTSLLKEIAHSVTTNHPEAELIVLLIDERPEEVTDIERSVKGDVVSSTFDEVPENHIKV
CHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AELVLERAMRLVEHKKDVIILMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFG
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHCCHHHHH
AARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRSLAERRIFPAIDIRR
HHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCEECCC
SGTRKEDLLIPKEHLDKLWGIRKTMRDTPDFVESFLRKLRQTKTNEEFLQNIVADSKRYV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
TTK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]