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The map label for this gene is nuoN

Identifier: 49480403

GI number: 49480403

Start: 5026693

End: 5028213

Strand: Reverse

Name: nuoN

Synonym: BT9727_4972

Alternate gene names: 49480403

Gene position: 5028213-5026693 (Counterclockwise)

Preceding gene: 49481258

Following gene: 49480402

Centisome position: 96.0

GC content: 38.92

Gene sequence:

>1521_bases
ATGGATATGAATACGTTACTTAGCTTATCGTGGCATCTCATGGTGCCAGAATTCATCATTCTCGGGGCTGCCATCCTTCT
TTCCATATGTGATTTGTTTTTTAAGCTGAACCATAGATACGTAGCGCTTGGTGCAATCGCGGCGGTCGTATTAGCAATCG
TGTCACTAATTACGCTATATAGCGAACCAGCGGGAGATATTTTAAATGGATCGTTTGTGTTAGATGGATTTTCAAAAGGA
TTTAAAACGTTGTTGTTAGGCGGGGCAGCCCTTATTTTGTGCACCGCAATGAGCGATGATAAGAAAAATCCAATTGAAGA
TAAGGGAGAGTATTATTACTTATTTTTAATGGCGCTTCTTGGGGCGATGTTTATGGCTTCTAGCGTTGATTTCGTTACAC
TTTTCGTCGGTTTAGAATTGCTTTCACTTTCTTCCTACATTTTAGTAGGAATACGAAAAAAGAACCTTGCATCAAATGAG
GCAGCAATGAAATATGTCATTAATGGAGGAATTGGAACGGCAATTACACTCTTTGGAATGAGTTACTTATACGGTATTAC
AGGGTCAACTAATATCGTGGATATGCAAAAAGTATTTGCCGGGGAACTGGCTAGTGGCATTCAGTTATTGCTAGCTCTCG
CATTCCTACTCTTACTCGTTGGGCTCTCATTCAAAATTGCGACAGTGCCGTTTCATATGTGGGCACCTGATGTGTATGAG
GGAGCGGCTACACCTGTTACTGCTTTTCTTGGAACAATTTCTAAAATGGCGGGTTTCTTACTCATTATTCGTTTGTTCCT
CATGGTTTTTGCAAGTGTATCGGTGCAAGGAGACATGCAATCTTTATACGGGCGTATGAGCATATATATCGCTGTGCTAG
CTAGTATTACGATGATTATTGGAAATGTAGTCGCATTAAAGCAATATAATGTAAAACGCCTATTTGCTTATTCGGGAATT
GCGCATGCGGGATATTTACTCGTTCCGCTTGTCGCATTATCACCATTTACGATGGATAGTATGTGGTTTTACATGCTTGC
GTATATGCTTATGAATATAGGCGCGTTTGCAATTATTCACGGCTTAATCTTACAAAGCGATAAAGAAAATATCACCATTT
TCACTGGATTATATAAAAGGTCGCCATTTACAGCGATTGTGATGACGATTTTTATTTTATCGCTAGCGGGGATACCAGGA
ACAGCTGGTTTTATTGGGAAAATTAACATCTTTTTAGGGGCTCTTCATGTAGAGCCAGCTCATTACGTACTAGCTTCTAT
TATGATGGGGACGACAGTCATTTCATTCGTATATTATTTCCGTATTTTACAACAAATGTTTTTCAGGACGGGAGAAGTAG
AAGAGAAAATTCGCTTGCCACTCAATATAAAGATTGTGATGAGCTTTTGTGCAATTTCGATTGTAATACTAGGGATTATG
CCGATGATTGGATACAATTTCTTTTATGAATATTTTCCATTAATGAAAGATTTCTTCTTCTTAGGGAACGTGGTACAATA
G

Upstream 100 bases:

>100_bases
ATCTTGCTACTTATCTTCTGCATTATTGCAATTGGCGTAATGCCAGAAATACTAGGGGATCCGCTTCAAAATACATTGAA
AACATTGGGGGTGAAGTAGG

Downstream 100 bases:

>100_bases
TGAAAATAAAAGGTGTGGAGTCGTTACTCTGCGCTTTTTATTTTGTGTGAACTACAATTGAAAATACATATAGGTATACG
TTTTTAAAGAGAAAAATTTC

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAVVLAIVSLITLYSEPAGDILNGSFVLDGFSKG
FKTLLLGGAALILCTAMSDDKKNPIEDKGEYYYLFLMALLGAMFMASSVDFVTLFVGLELLSLSSYILVGIRKKNLASNE
AAMKYVINGGIGTAITLFGMSYLYGITGSTNIVDMQKVFAGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYE
GAATPVTAFLGTISKMAGFLLIIRLFLMVFASVSVQGDMQSLYGRMSIYIAVLASITMIIGNVVALKQYNVKRLFAYSGI
AHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIHGLILQSDKENITIFTGLYKRSPFTAIVMTIFILSLAGIPG
TAGFIGKINIFLGALHVEPAHYVLASIMMGTTVISFVYYFRILQQMFFRTGEVEEKIRLPLNIKIVMSFCAISIVILGIM
PMIGYNFFYEYFPLMKDFFFLGNVVQ

Sequences:

>Translated_506_residues
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAVVLAIVSLITLYSEPAGDILNGSFVLDGFSKG
FKTLLLGGAALILCTAMSDDKKNPIEDKGEYYYLFLMALLGAMFMASSVDFVTLFVGLELLSLSSYILVGIRKKNLASNE
AAMKYVINGGIGTAITLFGMSYLYGITGSTNIVDMQKVFAGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYE
GAATPVTAFLGTISKMAGFLLIIRLFLMVFASVSVQGDMQSLYGRMSIYIAVLASITMIIGNVVALKQYNVKRLFAYSGI
AHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIHGLILQSDKENITIFTGLYKRSPFTAIVMTIFILSLAGIPG
TAGFIGKINIFLGALHVEPAHYVLASIMMGTTVISFVYYFRILQQMFFRTGEVEEKIRLPLNIKIVMSFCAISIVILGIM
PMIGYNFFYEYFPLMKDFFFLGNVVQ
>Mature_506_residues
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAVVLAIVSLITLYSEPAGDILNGSFVLDGFSKG
FKTLLLGGAALILCTAMSDDKKNPIEDKGEYYYLFLMALLGAMFMASSVDFVTLFVGLELLSLSSYILVGIRKKNLASNE
AAMKYVINGGIGTAITLFGMSYLYGITGSTNIVDMQKVFAGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYE
GAATPVTAFLGTISKMAGFLLIIRLFLMVFASVSVQGDMQSLYGRMSIYIAVLASITMIIGNVVALKQYNVKRLFAYSGI
AHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIHGLILQSDKENITIFTGLYKRSPFTAIVMTIFILSLAGIPG
TAGFIGKINIFLGALHVEPAHYVLASIMMGTTVISFVYYFRILQQMFFRTGEVEEKIRLPLNIKIVMSFCAISIVILGIM
PMIGYNFFYEYFPLMKDFFFLGNVVQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=479, Percent_Identity=33.4029227557411, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788831, Length=309, Percent_Identity=27.5080906148867, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788613, Length=382, Percent_Identity=23.8219895287958, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788614, Length=305, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788827, Length=417, Percent_Identity=23.7410071942446, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55587; Mature: 55587

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAVVLAIVSLITLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SEPAGDILNGSFVLDGFSKGFKTLLLGGAALILCTAMSDDKKNPIEDKGEYYYLFLMALL
CCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHH
GAMFMASSVDFVTLFVGLELLSLSSYILVGIRKKNLASNEAAMKYVINGGIGTAITLFGM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHEECCHHHHHHHHHH
SYLYGITGSTNIVDMQKVFAGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHC
GAATPVTAFLGTISKMAGFLLIIRLFLMVFASVSVQGDMQSLYGRMSIYIAVLASITMII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GNVVALKQYNVKRLFAYSGIAHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIH
HHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLILQSDKENITIFTGLYKRSPFTAIVMTIFILSLAGIPGTAGFIGKINIFLGALHVEPA
HHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHCCCHH
HYVLASIMMGTTVISFVYYFRILQQMFFRTGEVEEKIRLPLNIKIVMSFCAISIVILGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
PMIGYNFFYEYFPLMKDFFFLGNVVQ
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAVVLAIVSLITLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SEPAGDILNGSFVLDGFSKGFKTLLLGGAALILCTAMSDDKKNPIEDKGEYYYLFLMALL
CCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHH
GAMFMASSVDFVTLFVGLELLSLSSYILVGIRKKNLASNEAAMKYVINGGIGTAITLFGM
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHEECCHHHHHHHHHH
SYLYGITGSTNIVDMQKVFAGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHC
GAATPVTAFLGTISKMAGFLLIIRLFLMVFASVSVQGDMQSLYGRMSIYIAVLASITMII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GNVVALKQYNVKRLFAYSGIAHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIH
HHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLILQSDKENITIFTGLYKRSPFTAIVMTIFILSLAGIPGTAGFIGKINIFLGALHVEPA
HHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHCCCHH
HYVLASIMMGTTVISFVYYFRILQQMFFRTGEVEEKIRLPLNIKIVMSFCAISIVILGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
PMIGYNFFYEYFPLMKDFFFLGNVVQ
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]