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The map label for this gene is sigL

Identifier: 49480372

GI number: 49480372

Start: 4871644

End: 4872951

Strand: Reverse

Name: sigL

Synonym: BT9727_4821

Alternate gene names: 49480372

Gene position: 4872951-4871644 (Counterclockwise)

Preceding gene: 49481131

Following gene: 49479339

Centisome position: 93.04

GC content: 38.38

Gene sequence:

>1308_bases
TTGAAGGCAAGTCTTTTACAAGAACAAAGCTTACGTTTGGCAATGACACAAGAGTTAAGGCAAGCGATTACAATGCTCCA
GTATAATGTACAAGAATTATCGGAGTTTTTGTATGAGCAATCGTTAGAGAATCCCCTTATTGAGTTAGGTGGTTTTGAGA
GGGAGAAGAAAAAGAGCTCTAACAACAGTAAAAGTACCAGCAAACAAGTCGAGAATCAGATGGAAATCTACAGTGTGGAT
TCAACAACGATTCAGCAACATTTATTAAATCAAATACAGTATTATAAAATAGAGGAAGAAGAGCGAAAAGTAGCTTCTTT
CATTATTATGAACATGGATGGAAATGGGTATTTACAAGAAACGAATGAAGAGTTAGCAAATCTTCTTTCCGCACCACTGC
ATGTAGTCGACCGCTCTGTAGAGCTTGTCCAATCACTCGAGCCAGCAGGGGTAGGGGCGCGTAATATTCAGGAATGTCTA
ACCTTACAATTGAAGCGCTTACAAAGACGGAATGGATTAGCGGAGGAGGTTATAGAGGATCACTTTGCTTATTTCGTGAA
GAAAGATTGGCGAAAGCTCGCTCAAGTGTTGAAGTGTAGTAATGAAGAATTGCAGGATGCGGTAAATTGTATAACGTCAT
TACAACCAAAACCAGGTCTTGCGTTTTCTTCTGATAAACCACTTTATATTGTGCCTGATATGGCGGTGAAAAAAGATGGC
GATCGTCTCGTTTTACAAATGAACGAGAGAAATATGCCAAGAATTGAAATTCATTCTGAATATAGTGCGCTTCTCAATAA
TAGTGAAAGTGAAGTAGCATCTTACGTATCAGAAAAGTATCAGCATGTGCAGTGGATTATGCGTAGTTTAAAACAGAGAA
AACAAACCCTTCTGCAAGTGATGACCATTATTATGGAAAAACAACGTGATTTCTTCTGGAAAGGTCCGGAGTATTTAAAG
CCACTTGCTTTAAAAGAAGTAGCAGAAGAGTTAAGCGTGCATGAATCGACAATTAGTCGTGCAACGCGAAATAAATATGT
GCAAACACCACACGGTTTATTTGAGATGAAATCGTTCTTTAGTAATGCCGTTTCGACTACTGAAGATGAAGCAGTTTCTA
CAAAGCGTGTAAAACAATTTATTCAAACGCTTGTTGAGGCAGAGAATAAGAAAAAGCCACTTTCAGATCAAAAGATTTCA
AAATTGTTAGAAGAAGAGCATGAAATCGTTATTTCTCGAAGAACAGTGGCGAAGTATAGAGAGCAAATGCACATTCCAGC
GTCGTCATTACGAAAAACGATCGGATAG

Upstream 100 bases:

>100_bases
ATTAATACATATAAATTAATAATATTTTGGAATGGGTGTCTGTGATAGGATTCTCCTTTTACCAAACTTATTGTACAATA
GAAACAAGGGGGAGATTAGT

Downstream 100 bases:

>100_bases
GTGAAGAAGATGAAAGTTGTACTGTATACAAAAAAAGATTGTGGCCTTTGCGTGAAGGCGAAACAAGTTTTACAGGAAGT
ACAATGTGAATATTCTTTTC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNNSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIQECL
TLQLKRLQRRNGLAEEVIEDHFAYFVKKDWRKLAQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPEYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG

Sequences:

>Translated_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNNSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIQECL
TLQLKRLQRRNGLAEEVIEDHFAYFVKKDWRKLAQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPEYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG
>Mature_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNNSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIQECL
TLQLKRLQRRNGLAEEVIEDHFAYFVKKDWRKLAQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPEYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=476, Percent_Identity=32.7731092436975, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50310; Mature: 50310

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
NNSKSTSKQVENQMEIYSVDSTTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQE
CCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHH
TNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIQECLTLQLKRLQRRNGLAEEVIED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HFAYFVKKDWRKLAQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCC
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTIIMEKQRDFFWKGPEYLKPLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKISKLLEEEHEIVISRRTVAKYR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
NNSKSTSKQVENQMEIYSVDSTTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQE
CCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHH
TNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIQECLTLQLKRLQRRNGLAEEVIED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HFAYFVKKDWRKLAQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCC
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTIIMEKQRDFFWKGPEYLKPLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKISKLLEEEHEIVISRRTVAKYR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]