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The map label for this gene is pcaD

Identifier: 49480117

GI number: 49480117

Start: 3155370

End: 3156272

Strand: Reverse

Name: pcaD

Synonym: BT9727_3084

Alternate gene names: 49480117

Gene position: 3156272-3155370 (Counterclockwise)

Preceding gene: 49478154

Following gene: 49480602

Centisome position: 60.26

GC content: 36.32

Gene sequence:

>903_bases
ATGATTAAGCCTGCAACAATGGAGTTTGTTTCACTATCGAACGGAGAAACGATTGCGTATCAGGAAGTTGGAAGGCAAAA
TGCAGAAATTCTTGTACTCATTCACGGGAACATGACATCGTCACAACACTTTGATTTAGTTATTGAAAAGCTGCAAGATC
AATACCATATTTACGCTCTTGATTTAAGAGGATTTGGTCAATCAACATATAATAAACCGATAGATTCAATACAAGACTTT
GCAGAAGATGTAAAACTATTTATCGACGGGTTAAAACTAGAGAAATTTTCATTAATGGGCTGGTCAATGGGCGGTGGTGT
TGCGATGCAATTTACAGCGAATCATCCAACGTTTGTCGAAAAGTTAATTTTAGTAGAATCAGTAGGAATGAAGGGCTATC
CAATCTTTAAAAAAGATATAAACGGGCAGCCGATTGTATCTAGTTTAGTTAAGACAAAAGAGGAAATTGCGCAAGATCCT
GTACAAATTGCACCAGTGTTAGATGCGATAAAAAATATGAATAAACTATATTATCGTACAGTATGGAATTTGTTAATATA
TACACATAATCAACCTGAACCGGATCGCTATGAAAAGTATTTAGACGATATGTTAACGCAGCGTAATTTCGTAGATGTGA
ATTATGCGCTCATTACATTTAATATTTCTGATGAACATAACGGGGTTGTAGAGGGAAATAAGCAAATTCATCGTATTAAA
GCTCCGACACTCGTCATACAAGGTGACAGAGATTATGTTGTACCGCAAGTAGTGGGTGAGGAGTTAGCAAAACATTTGCC
GAATGCAGAGCTGAAGATATTAGAAGATTGCGGACACTCACCGTTTATAGATTGCTTAGATGTATTTATTAAGCATGTAG
AGGATTGGTTAGAAGGTAAGTAG

Upstream 100 bases:

>100_bases
AATTTCGTATAGCTTTTATGTTGAGAACATACATTCTGCATGTTATTCTTTAATTAAGTAAAGAATTTTCTTTCTATTAA
AAAGAATGGGGGAACTTGTG

Downstream 100 bases:

>100_bases
GGGGAGGAAGAAGTTAGATGACATGTGTCTAGCTTCTTTTTTGTGGAAAAATGAAAATTATTTCATCCACTAGTTCTTTC
TTATTACATAGCAAACAATA

Product: 3-oxoadipate enol-lactonase

Products: 3-oxoadipate

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIKPATMEFVSLSNGETIAYQEVGRQNAEILVLIHGNMTSSQHFDLVIEKLQDQYHIYALDLRGFGQSTYNKPIDSIQDF
AEDVKLFIDGLKLEKFSLMGWSMGGGVAMQFTANHPTFVEKLILVESVGMKGYPIFKKDINGQPIVSSLVKTKEEIAQDP
VQIAPVLDAIKNMNKLYYRTVWNLLIYTHNQPEPDRYEKYLDDMLTQRNFVDVNYALITFNISDEHNGVVEGNKQIHRIK
APTLVIQGDRDYVVPQVVGEELAKHLPNAELKILEDCGHSPFIDCLDVFIKHVEDWLEGK

Sequences:

>Translated_300_residues
MIKPATMEFVSLSNGETIAYQEVGRQNAEILVLIHGNMTSSQHFDLVIEKLQDQYHIYALDLRGFGQSTYNKPIDSIQDF
AEDVKLFIDGLKLEKFSLMGWSMGGGVAMQFTANHPTFVEKLILVESVGMKGYPIFKKDINGQPIVSSLVKTKEEIAQDP
VQIAPVLDAIKNMNKLYYRTVWNLLIYTHNQPEPDRYEKYLDDMLTQRNFVDVNYALITFNISDEHNGVVEGNKQIHRIK
APTLVIQGDRDYVVPQVVGEELAKHLPNAELKILEDCGHSPFIDCLDVFIKHVEDWLEGK
>Mature_300_residues
MIKPATMEFVSLSNGETIAYQEVGRQNAEILVLIHGNMTSSQHFDLVIEKLQDQYHIYALDLRGFGQSTYNKPIDSIQDF
AEDVKLFIDGLKLEKFSLMGWSMGGGVAMQFTANHPTFVEKLILVESVGMKGYPIFKKDINGQPIVSSLVKTKEEIAQDP
VQIAPVLDAIKNMNKLYYRTVWNLLIYTHNQPEPDRYEKYLDDMLTQRNFVDVNYALITFNISDEHNGVVEGNKQIHRIK
APTLVIQGDRDYVVPQVVGEELAKHLPNAELKILEDCGHSPFIDCLDVFIKHVEDWLEGK

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.24

Molecular weight: Translated: 34159; Mature: 34159

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPATMEFVSLSNGETIAYQEVGRQNAEILVLIHGNMTSSQHFDLVIEKLQDQYHIYAL
CCCCCCEEEEECCCCCEEEHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
DLRGFGQSTYNKPIDSIQDFAEDVKLFIDGLKLEKFSLMGWSMGGGVAMQFTANHPTFVE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEECCCHHHHH
KLILVESVGMKGYPIFKKDINGQPIVSSLVKTKEEIAQDPVQIAPVLDAIKNMNKLYYRT
HHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VWNLLIYTHNQPEPDRYEKYLDDMLTQRNFVDVNYALITFNISDEHNGVVEGNKQIHRIK
HHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEECCCCEEEEEE
APTLVIQGDRDYVVPQVVGEELAKHLPNAELKILEDCGHSPFIDCLDVFIKHVEDWLEGK
CCEEEEECCCCEECHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKPATMEFVSLSNGETIAYQEVGRQNAEILVLIHGNMTSSQHFDLVIEKLQDQYHIYAL
CCCCCCEEEEECCCCCEEEHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
DLRGFGQSTYNKPIDSIQDFAEDVKLFIDGLKLEKFSLMGWSMGGGVAMQFTANHPTFVE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEECCCHHHHH
KLILVESVGMKGYPIFKKDINGQPIVSSLVKTKEEIAQDPVQIAPVLDAIKNMNKLYYRT
HHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VWNLLIYTHNQPEPDRYEKYLDDMLTQRNFVDVNYALITFNISDEHNGVVEGNKQIHRIK
HHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEECCCCEEEEEE
APTLVIQGDRDYVVPQVVGEELAKHLPNAELKILEDCGHSPFIDCLDVFIKHVEDWLEGK
CCEEEEECCCCEECHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxoadipate enol-lactone; H2O

Specific reaction: 3-oxoadipate enol-lactone + H2O = 3-oxoadipate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]