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The map label for this gene is phnD

Identifier: 49479435

GI number: 49479435

Start: 3525950

End: 3526888

Strand: Reverse

Name: phnD

Synonym: BT9727_3441

Alternate gene names: 49479435

Gene position: 3526888-3525950 (Counterclockwise)

Preceding gene: 49479436

Following gene: 49481743

Centisome position: 67.34

GC content: 34.4

Gene sequence:

>939_bases
ATGTTGAAAAAAGTTTTTGCAATGAGCACAACAGTTGTACTAGCAGCGGGGGTATTAAGTGGCTGTGGAACGAAGGAATC
AAGTGCAAATAAAAATGCAGATACGAAAAAAGGGTATGTACCGAAGACTTTAAATGTTCAATTTGTTCCTTCTCAAAATG
CAGATACGTTAGAGGCGAAGGCAAAACCACTTGAGAAGCTATTAAGTGATAAGTTAAATATTCCAGTAAAAGTTAGTATT
TCAACGAATTACAATACAATTGTAGAAGCGATGGCATCTAAGCAAGTAGATGTAGGATTTTTACCCCCAACAGCGTATGT
GTTAGCACATGAGAAAAATGCAGCAAACGTAATTTTACAAGCACAACGTTTTGGAGTAGATGATGAAACCGGAGCTCCTA
CAAAAGATTTAGTAGACTTCTATAAATCTGAGTTTGTTGTTAAGAAAGATTCAAATATTAATAGTGTAAAAGATTTAAAA
GATAAAAAAATTGGTTATCAAGATGTAACGTCATCAGCGGGATATGTGTGGCCTGCGGCTGTATTATTAAAAGAGGGCGT
TGATCCGTTAAAAGATGTGAAACCTGTTACATTAAAAGGTCACGATCAGTCGCTTATTGCTCTTTTAAATGGTGACGTAG
ATGCAGCTGTTGTATTCCAAGATGCTCGTAATATTGTAAAAAAAGATTATCCAAATATATTCGATCAAACGAAAATAGTG
AAATTTACGGAGAAAATTCCAAACGATACGATTTCGGTACGTTCGGATTTAGATGAAGAGTGGAGCAAGAAATTACAGGA
TGCTTTCATTGAAATTGGAAAGGATGAGGAAGGTCATAAAATCATTAAAGAAGTATATTCACATGAAGGATATGTTAAAT
CTGATGATAGTAAATTTGATATCGTTCGTGAATATGGAAAGAAAGTAAAAACGCAATAA

Upstream 100 bases:

>100_bases
AAAGATTCTGTAAATAATCAAATTTTTGTAAAAATGTGTGATATATTTCTTCATGTTTATGAAAGGTTATTTTATAACAA
TGAAAAATGGAGGCACTAAT

Downstream 100 bases:

>100_bases
TACATATGGAAATGGCAGCTAGCTGGAAAGGTATGTTTTCAGTTAGTTTGCCGTTTTGAATTCATAGAAGGTAGGTAAGA
TGTGTGATAGAGTTTCGAAA

Product: phosphonate ABC transporter phosphate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLKKVFAMSTTVVLAAGVLSGCGTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSI
STNYNTIVEAMASKQVDVGFLPPTAYVLAHEKNAANVILQAQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLK
DKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIV
KFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKDEEGHKIIKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ

Sequences:

>Translated_312_residues
MLKKVFAMSTTVVLAAGVLSGCGTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSI
STNYNTIVEAMASKQVDVGFLPPTAYVLAHEKNAANVILQAQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLK
DKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIV
KFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKDEEGHKIIKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ
>Mature_312_residues
MLKKVFAMSTTVVLAAGVLSGCGTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAKAKPLEKLLSDKLNIPVKVSI
STNYNTIVEAMASKQVDVGFLPPTAYVLAHEKNAANVILQAQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLK
DKKIGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIV
KFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKDEEGHKIIKEVYSHEGYVKSDDSKFDIVREYGKKVKTQ

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for phosphate binding [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790543, Length=267, Percent_Identity=25.0936329588015, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 34433; Mature: 34433

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKVFAMSTTVVLAAGVLSGCGTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
AKPLEKLLSDKLNIPVKVSISTNYNTIVEAMASKQVDVGFLPPTAYVLAHEKNAANVILQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEE
AQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLKDKKIGYQDVTSSAGYVWPAA
HHHCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCCHHHHCCCCCCHHHHH
VLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIV
HHHHCCCCHHHHCCCEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHCCCCHHCCHHHH
KFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKDEEGHKIIKEVYSHEGYVKSDDSKFD
HHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHH
IVREYGKKVKTQ
HHHHHHHHCCCC
>Mature Secondary Structure
MLKKVFAMSTTVVLAAGVLSGCGTKESSANKNADTKKGYVPKTLNVQFVPSQNADTLEAK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
AKPLEKLLSDKLNIPVKVSISTNYNTIVEAMASKQVDVGFLPPTAYVLAHEKNAANVILQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEE
AQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKDLKDKKIGYQDVTSSAGYVWPAA
HHHCCCCCCCCCCHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCCHHHHCCCCCCHHHHH
VLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDARNIVKKDYPNIFDQTKIV
HHHHCCCCHHHHCCCEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHCCCCHHCCHHHH
KFTEKIPNDTISVRSDLDEEWSKKLQDAFIEIGKDEEGHKIIKEVYSHEGYVKSDDSKFD
HHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHH
IVREYGKKVKTQ
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA