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The map label for this gene is colA [H]

Identifier: 49479179

GI number: 49479179

Start: 3584782

End: 3586623

Strand: Reverse

Name: colA [H]

Synonym: BT9727_3502

Alternate gene names: 49479179

Gene position: 3586623-3584782 (Counterclockwise)

Preceding gene: 49480843

Following gene: 49479426

Centisome position: 68.48

GC content: 34.96

Gene sequence:

>1842_bases
ATGAAAATATTTCAAAAGATGTGTGCGTCAACTGTAATCATTACAACACTTTTAGGAATAGGAGGTACTGTCAATAGTGT
ACATGCTGATTCTATAGAACAGCAGAGTAAATTAGGAATAGACCAAGAAACAGATAAGCAAATCATTGTAAAATTTAAAG
CGGATTTGAAATTTCCATATGAAGATGGTATTGAAAAACAAATACAATCTCAAACGAACGACAAAGTACTAGAAGATCTT
TTGGTAGAATATCCAGATGTAACATTCACGCGCTTATTCACATCTGTAAGTCCAGAGGAAATAGAAGACCTTGAAGTGAA
AGCACCCAATAATGTATCTACAAGTTTATTAAACTACTATATTCTTCAAACTCAAAATGGTGAGCAGAAAGAAGAACTAG
TAGATAGGTTAAAGAAATCTTGTTTAATAGAAGATGTGTATATGAAGAAACAAGAAAAAATAACGCCACCAGAAGTGCAA
CCATCAAGTGTATCTCTTAGTCCTAATAACAATCCTAGATTTAAACAACAAGGTTATTTAGAAGCAGCACCATACGGTAT
TAATGCGCCCTTCGCCTGGGAAGTTCAAGGCGGTAAAGGAAGTGGTATAACTTTTGTTGATATGGAATATGGATGGTTAT
TAAATCATGAGGATTTAGTACATCAAAATATTGAATTAATGTCTGGAATAAATATAGATCAACATGTCGGTCATGGGACG
TCTGTACTAGGAATTGTATCCTCTGAGGACAATGAAGTTGGGAATATTGGGATTGCGCCAAAAGCGAAAGCAAAGGTGAT
TTCTCAAATAAGAGATAACGGACAGTATAATACAGCTGATGCAATATTAAGTGCTGTGAATCAGTTAGAAGCTGGGGATG
TTTTATTATTAGAGGCACAAGCTTCCTTTGATGGATATGGTGATAAATATTTACCTGTTGAGGTACAACCAGATATCTTT
GATGCAATTCGTGCCGGTACAGATAAAGGTGTTGTCATTATAGAAGCTGGAGCAAATGGTTGGAATGATTTAGACCAATT
TAAAGATAGAAATGGTAAACAAATTTTAAATCGTAATAGTAAAGATTTTAAAGATTCAGGTGCAATTATGGTAGGAGCGG
GCTCTTCATCTTTCCCTCATGAGCGTATGTGGTTTTCGAATTATGGCAGCCGTATTGATGTGTATGGATGGGGAGAAAAC
GTAGATACAACTACAGCAGAGCAAAGTCGAAGTGCTGTAAATCTTTATACATCTAGCTTTAGTGGCACGTCGAGTGCGTC
GCCTATTATTGCCGGAGCAGCGACTTTAGTACAAAGCATTGCGAAAGAAAATTTAGGACAACCATATAGACCGAGTGAAC
TAAGAGCAATATTAAGTAACGAGAACACAGGAACAAAATCTAAAGATCCATATGCAGATCAAATTGGTGTTTTACCAGAT
TTGAAGTCTATACTAGTAAACTTAGGGTATGGACAAAGAAAACCAAACGGTGGGAATGAGTTACAAGTAACAGAAAATGA
ACCAAATAATGAACGTATACAGGCAAATAAAGTTAACTTTCATACACCGATGAAAGGGACACTACATAATAGTGATAGAG
TAGATGTATTTACTTTCCAAATGGACTCACCAGAAAATATTACTATTTCTCTACTAAATGAACAAAACATTGGGATGACA
TGGGTGCTTCATCATGAATCAGATTTAAATAACTATGTAGCATATGGTGAAAATGAAGGAAATGTAGTGAAAGGTACTTA
TCATGCAAAGCCAGGTAAATATTATTTATACGTCTATAAGTATGAGAATAAAGATGGTTCATATGTATTGAATATAAAGT
AA

Upstream 100 bases:

>100_bases
ATTACTATAATCTATTTAGTAGAAAGGGAATATGTTATATAAATAAAATCATTATAAATTAATATAATGATTAATTGTTG
TTAATAGGAGGATTGATGTA

Downstream 100 bases:

>100_bases
ACGAAAGGAACTTCAGATGAAGTTCCTTTAAATTTTTCTAACTCAGGATATGTGAATGATTTTTTAACTTTACTTCTATG
CAATATTTCATATTTATGGA

Product: serine protease

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKIFQKMCASTVIITTLLGIGGTVNSVHADSIEQQSKLGIDQETDKQIIVKFKADLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDVTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSCLIEDVYMKKQEKITPPEVQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNENTGTKSKDPYADQIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPMKGTLHNSDRVDVFTFQMDSPENITISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYHAKPGKYYLYVYKYENKDGSYVLNIK

Sequences:

>Translated_613_residues
MKIFQKMCASTVIITTLLGIGGTVNSVHADSIEQQSKLGIDQETDKQIIVKFKADLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDVTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSCLIEDVYMKKQEKITPPEVQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNENTGTKSKDPYADQIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPMKGTLHNSDRVDVFTFQMDSPENITISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYHAKPGKYYLYVYKYENKDGSYVLNIK
>Mature_613_residues
MKIFQKMCASTVIITTLLGIGGTVNSVHADSIEQQSKLGIDQETDKQIIVKFKADLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDVTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSCLIEDVYMKKQEKITPPEVQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNENTGTKSKDPYADQIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPMKGTLHNSDRVDVFTFQMDSPENITISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYHAKPGKYYLYVYKYENKDGSYVLNIK

Specific function: Unknown

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 67809; Mature: 67809

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFQKMCASTVIITTLLGIGGTVNSVHADSIEQQSKLGIDQETDKQIIVKFKADLKFPY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVLEDLLVEYPDVTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCEEEECCCCHHHHEEEEE
ILQTQNGEQKEELVDRLKKSCLIEDVYMKKQEKITPPEVQPSSVSLSPNNNPRFKQQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHHCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEE
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNENTGTKSKDPYADQIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPMKGTLHNSDRVDVFTFQ
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEE
MDSPENITISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYHAKPGKYYLYVYK
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC
>Mature Secondary Structure
MKIFQKMCASTVIITTLLGIGGTVNSVHADSIEQQSKLGIDQETDKQIIVKFKADLKFPY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVLEDLLVEYPDVTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCEEEECCCCHHHHEEEEE
ILQTQNGEQKEELVDRLKKSCLIEDVYMKKQEKITPPEVQPSSVSLSPNNNPRFKQQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHHCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEE
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNENTGTKSKDPYADQIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPMKGTLHNSDRVDVFTFQ
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEE
MDSPENITISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYHAKPGKYYLYVYK
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]