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The map label for this gene is nsr [H]

Identifier: 49478992

GI number: 49478992

Start: 3295653

End: 3296345

Strand: Reverse

Name: nsr [H]

Synonym: BT9727_3228

Alternate gene names: 49478992

Gene position: 3296345-3295653 (Counterclockwise)

Preceding gene: 49479488

Following gene: 49478240

Centisome position: 62.94

GC content: 31.02

Gene sequence:

>693_bases
ATGAAGAAAGTATGTAATTCTATTAAATTAGCAGAGTATATGAAACAAAATAATATTGACTCCTTTTTTAGTAATGATAT
GAATCCGTATATGGAACTTATATTTTTTAAAAAGAATGAGTTTATTTGTAGAGAAAATGAAGAGATAGACTATTTATATT
TTTTCGTTGAAGGAAAAGCGAAGGCATTTAATACATTAAGTAACGGAAAATCTGTATTATTATGCTTTTATGATAGTTTG
CAGCTGTTAGGAGATGTGGAGTTAATTCATTCGCAAAAGACAGCTTCAAACGTACAAGTAATGGCAGATTCATATTGTGT
TGGTTTGCCTTTAGGAAAAGTGAGAAATCAACTATTTCATGATGCAAAATTTTTACGATGTATTTGCGGATCATTAGCAC
ATAAGTTAAATCGACTTTCCAAAAATAGTACAATTAATTTACTATATCCGCTTGAAAATAGACTTGCGAGCTACATGTTA
GCGGCAGGAGAAAGAGCAGCTCAGCATGGAAATCGAATTGTGTTTAGTGGAAATTTAACGGAAACAGCTGAATTGTTAGG
AACGAGTTATCGGCACCTATTACGTACATTAAATATTTTTTGTGATAAAGAGATTATAAAGAAAAATAATGGTTGTTTTG
AAGTAGTGAATGTGGATGTTTTGAGGGAATTGGCGGCAGATGTTTATAAATAG

Upstream 100 bases:

>100_bases
GACTGTATCATGCTGAAATTGTGTATTACTATGACATATGTCATAGTGGAAGAAGAAAATTTTGATTACAATGGGGATGA
GTTGTAAAGGTGGGGGAACG

Downstream 100 bases:

>100_bases
GAGGAGAGTTTAAATGATTACACCTATATTTAGAATTTTTGATATTGAAAAAGCAAAGCTATTTTACTTAGGTTTTTTAG
GATTTAAACTGGATTGGGAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKKVCNSIKLAEYMKQNNIDSFFSNDMNPYMELIFFKKNEFICRENEEIDYLYFFVEGKAKAFNTLSNGKSVLLCFYDSL
QLLGDVELIHSQKTASNVQVMADSYCVGLPLGKVRNQLFHDAKFLRCICGSLAHKLNRLSKNSTINLLYPLENRLASYML
AAGERAAQHGNRIVFSGNLTETAELLGTSYRHLLRTLNIFCDKEIIKKNNGCFEVVNVDVLRELAADVYK

Sequences:

>Translated_230_residues
MKKVCNSIKLAEYMKQNNIDSFFSNDMNPYMELIFFKKNEFICRENEEIDYLYFFVEGKAKAFNTLSNGKSVLLCFYDSL
QLLGDVELIHSQKTASNVQVMADSYCVGLPLGKVRNQLFHDAKFLRCICGSLAHKLNRLSKNSTINLLYPLENRLASYML
AAGERAAQHGNRIVFSGNLTETAELLGTSYRHLLRTLNIFCDKEIIKKNNGCFEVVNVDVLRELAADVYK
>Mature_230_residues
MKKVCNSIKLAEYMKQNNIDSFFSNDMNPYMELIFFKKNEFICRENEEIDYLYFFVEGKAKAFNTLSNGKSVLLCFYDSL
QLLGDVELIHSQKTASNVQVMADSYCVGLPLGKVRNQLFHDAKFLRCICGSLAHKLNRLSKNSTINLLYPLENRLASYML
AAGERAAQHGNRIVFSGNLTETAELLGTSYRHLLRTLNIFCDKEIIKKNNGCFEVVNVDVLRELAADVYK

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=215, Percent_Identity=30.2325581395349, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 26270; Mature: 26270

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVCNSIKLAEYMKQNNIDSFFSNDMNPYMELIFFKKNEFICRENEEIDYLYFFVEGKA
CCHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEEEECCCEEECCCCCCEEEEEEEECCH
KAFNTLSNGKSVLLCFYDSLQLLGDVELIHSQKTASNVQVMADSYCVGLPLGKVRNQLFH
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCHHHHHHHHHH
DAKFLRCICGSLAHKLNRLSKNSTINLLYPLENRLASYMLAAGERAAQHGNRIVFSGNLT
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCH
ETAELLGTSYRHLLRTLNIFCDKEIIKKNNGCFEVVNVDVLRELAADVYK
HHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVCNSIKLAEYMKQNNIDSFFSNDMNPYMELIFFKKNEFICRENEEIDYLYFFVEGKA
CCHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEEEECCCEEECCCCCCEEEEEEEECCH
KAFNTLSNGKSVLLCFYDSLQLLGDVELIHSQKTASNVQVMADSYCVGLPLGKVRNQLFH
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCHHHHHHHHHH
DAKFLRCICGSLAHKLNRLSKNSTINLLYPLENRLASYMLAAGERAAQHGNRIVFSGNLT
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEECCCH
ETAELLGTSYRHLLRTLNIFCDKEIIKKNNGCFEVVNVDVLRELAADVYK
HHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]