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The map label for this gene is fadD

Identifier: 49478964

GI number: 49478964

Start: 3459187

End: 3460866

Strand: Reverse

Name: fadD

Synonym: BT9727_3380

Alternate gene names: 49478964

Gene position: 3460866-3459187 (Counterclockwise)

Preceding gene: 49478301

Following gene: 49481757

Centisome position: 66.08

GC content: 34.64

Gene sequence:

>1680_bases
GTGGGAGACGATGAAAGTGAGTGCTGTGTTTTTACCAGGAGATTGCATAAAAGCTATTATTGCAGCGATTCTTGTAACGA
AATTACATCGTTCATTGAAACATATTATTACGCCTGCTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAA
GAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTTGTAAAGAAAGGACATCTCATATGGGAATAACAAAAGAATATAAA
AAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTCTTAACATATAAAGAGTGGTTTGAGTC
AGTGTATAAAGTAGCAAACTGGTTGAATGAAAAAGAATCGAAGAATAAAACGATAGCAATTGTATTAGAAAATCGTATAG
AGTTTTTACAACTATTCGCTGGTGCTGCTATGGCAGGATGGGTTTGTGTGCCACTAGATATAAAGTGGAAACAAGATGAG
CTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGACAGAGAGATATAAGTTAAATGATTTACCGGATGAAGA
AGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTACATATTTCCCTATAGAGAATGTAC
AAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGGAAAGCAAAAGCGTTTTTACGTGCGCAACAATCGTGG
CTTCATAGTTTTGATTGTAATGTACATGACTTTCATATGAAAAGAGAGGATTCGATTTTAATAGCTGGGACGCTTGTTCA
TTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGACAAACGGTTCACATTATGAGAAAGTTCATTCCAAATC
AAGTACTAGATAAGTTAGAAACTGAAAATATATCAGTTATGTATACAGTTCCGACAATGCTTGAAGCTTTATATAAAGAA
AATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGCTGGAGCGAAAGAAAAAATAAAGAA
TATATTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTGTAACAGCATTAGTTGATGAAGAGA
GCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTATGTAATGAGGCAGGAAAAGAAGTA
CAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATACATAATAGATGGTGTTTTAGCACG
GGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAGAAGGATTTATTTATATTATCGGTA
GAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAAAGCGTATTACATGAACATCCAGCT
GTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGTCGCGATTGTAAAAGGAAGTGCTAC
GAGGCAACAATTAAAGCGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGAAAGAATGGTATTTTGTAGATGAAA
TACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCAAAGAGTATCATTGAAAATCAGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
TTTCTATTATAAAAGTATTATGCATTAATATAATTGGTGGTATTTTCGTAGTTTATGTATTTGGTATTACTGTACAAGCT
TTCTTAATGGGTGTTTCCGT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACTATGAAGTTCAGCAATTAG
CAGCACCGCTTCTTACATTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Sequences:

>Translated_559_residues
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE
>Mature_558_residues
GDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYKK
HASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDEL
KERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWL
HSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKEN
RVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQ
KGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAV
DEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=356, Percent_Identity=27.5280898876405, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=25, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=25, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311624, Length=495, Percent_Identity=23.4343434343434, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI157311622, Length=495, Percent_Identity=23.4343434343434, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=23.3532934131737, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=27.8169014084507, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI38505220, Length=353, Percent_Identity=25.2124645892351, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI122937307, Length=371, Percent_Identity=25.6064690026954, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI115511026, Length=495, Percent_Identity=23.030303030303, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI13375727, Length=427, Percent_Identity=20.8430913348946, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=24.5247148288973, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.0556660039761, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=24.5098039215686, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=25.5192878338279, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790505, Length=399, Percent_Identity=23.0576441102757, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=24.3445692883895, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=24.0986717267552, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=301, Percent_Identity=30.8970099667774, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=365, Percent_Identity=28.2191780821918, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.7617728531856, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=27.7472527472527, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=349, Percent_Identity=26.647564469914, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=24.4444444444444, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=490, Percent_Identity=23.6734693877551, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=24.040404040404, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=486, Percent_Identity=21.3991769547325, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=532, Percent_Identity=25, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=24.0213523131673, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=27.8592375366569, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=24.8971193415638, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=23.3400402414487, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.4979919678715, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356947, Length=229, Percent_Identity=26.6375545851528, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356441, Length=463, Percent_Identity=22.2462203023758, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=21.8934911242604, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358303, Length=182, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648255, Length=507, Percent_Identity=21.6962524654832, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=23.314606741573, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=25.2906976744186, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161076582, Length=205, Percent_Identity=27.8048780487805, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62472339, Length=405, Percent_Identity=25.679012345679, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667955, Length=405, Percent_Identity=25.679012345679, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667959, Length=405, Percent_Identity=25.679012345679, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=25.7281553398058, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648257, Length=199, Percent_Identity=25.1256281407035, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355225, Length=443, Percent_Identity=22.3476297968397, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 64848; Mature: 64717

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLEALYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLEALYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]