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The map label for this gene is alkK

Identifier: 49478963

GI number: 49478963

Start: 3461415

End: 3463028

Strand: Reverse

Name: alkK

Synonym: BT9727_3382

Alternate gene names: 49478963

Gene position: 3463028-3461415 (Counterclockwise)

Preceding gene: 49479448

Following gene: 49478301

Centisome position: 66.12

GC content: 39.1

Gene sequence:

>1614_bases
ATGATGATGAATGTACCGCTAACAATTAGTTCTATGATGGAAAGAGCAGAAAAACTGTTTTCGAAGAAAGAAATTGTTTC
ACGGACACATGACACAGTTACGACATTAACGTATAAGCAGTTAGGTGAAAGAACAAGAAGGCTTTCCAGTGCGTTAAAAA
AATTAGGAATTAAAGAAGGCGAGCGTATAGGAACGTTAGCGTGGAATCATCATCGACATGTGGAAGCGTATTTTGCTATT
CCTGGTATTGCTTCCGTTTTGCACACAATTAATATTCGTTTATCTCCTCAACATATTTCATACATTATTCAGCATGCAGA
AGATCGAATTCTACTTATTGATGAAGATCTTGTACCACTCATTGAAAATATTCAATCACAATTAGCAACTGTACAAGCCT
ACATTATTATGACTGATAAAGATGAACTTCCAAATACTACGCTAGAACCTGTATATCATTATGAAAAACTATTAGAAGAA
GGCGATCCAAATTTCCAATTTGTAAAAGATATTGATGAAAATACACCTGCTGGTATGTGTTATACGTCAGCGACTACAGG
AAATCCAAAAGGTGTTGTATATACGCATCGTAGTACTGTACTGCATTGTATGGCACTCGGTTTAGCAGATACAGCTGCTT
TATCGGAAAGTGATGCGGCAATGGCTATTGTACCGATGTTTCATGTGAATGCTTGGGGACTACCTTTCGCAGCTACTTGG
TTTGGGTCAAAACAAGTGCTTCCAGGACCGATGTTTACGCCGAAAATTTTATTAGAAATGATTCAAGCTGAAAAAGTGAC
GTTAGCTGCTGGTGTGCCGACAATTTGGCTTGGTGTATTACAAGAGTTAGAAAATAATAGTTACGATTTATCTAGTATGA
CGAGGATACTATGCGGTGGTGCAGCTGCACCGAAGAGTGTTATTAAAGCATTTGAACAGAAACATAATGTTCCGTTCATC
CATGCATATGGTATGACCGAAACAAGCCCACTCGTAACACTTGCACGTTTAAAAAGTTATGAAACAGAATTATCGTATGA
AGAGCAATTAGAAATTCGATCAAAACAAGGGTATCTTGTTCCTGGTGTAGAGATGAAAGTAGTCGGTACAAATGGTGAAG
TGAAGTGGGATAGTACGGAGATGGGAGAACTATGTTTACGAGCGCCATGGATCGCTGAAAGCTATTATAACGATGATCGT
ACTGTTGAAGGCTTTCGCGATGGATGGTTATATACAGGAGATGTTGTCACAGTCGATGAGGAAGGTTGCGTAAAAATTGT
CGACCGCACGAAAGATGTGATTAAAAGCGGAGGCGAGTGGATTTCCTCTGTTGACCTTGAAAATGCGTTAATGGCACATG
ATGCTATATTTGAAGCGGCTGTCGTTGCCGTTCCTCATCCGCAGTGGCAAGAGCGACCAGTTGCGTGTGTCGTTCAAAAG
AAAAATAGTACAGTAACAAAAGAAGAGATATATGAGTTTTTAAAACCACAGTTTGCAAAGTGGTGGTTACCAGACGATAT
TGTATTTATGGAAGAAATACCGAAAACATCTGTTGGGAAGTTTTTAAAGCAGGCGCTTCGGAAAGAACTCGAGCATTTGC
ATAAAGAAAAATAA

Upstream 100 bases:

>100_bases
GAAATAATTCAGAAAATACAGAAAAATGTCGTAATTATTTGTAGAAGTCTGTTATAATAAACGAAAAAATGAAAACGCTT
TAAAGGGGGATATAACCAAT

Downstream 100 bases:

>100_bases
TTGAATTTATATTTGTAAACTAATAAAGAAAACGAGTTGACAAAAGCTAAATAGAAAGATAATATATCTCCTATAATATG
TTACCTAAAAACCATTTTGA

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLATVQAYIIMTDKDELPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Sequences:

>Translated_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLATVQAYIIMTDKDELPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
>Mature_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLIENIQSQLATVQAYIIMTDKDELPNTTLEPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=556, Percent_Identity=28.0575539568345, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=25.7299270072993, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=25.7299270072993, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=24.950884086444, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=23.6328125, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=23.5621521335807, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=22.568093385214, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=22.568093385214, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=22.1153846153846, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.7898832684825, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=22.093023255814, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI83745141, Length=512, Percent_Identity=23.046875, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1788107, Length=522, Percent_Identity=26.0536398467433, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI145693145, Length=363, Percent_Identity=31.9559228650138, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=23.7816764132554, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1786810, Length=529, Percent_Identity=24.5746691871456, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789201, Length=507, Percent_Identity=23.2741617357002, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=539, Percent_Identity=22.6345083487941, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788595, Length=501, Percent_Identity=22.9540918163673, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=518, Percent_Identity=24.9034749034749, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=31.9444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=25.8252427184466, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=25.891181988743, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=25.891181988743, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=25.891181988743, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=25.5597014925373, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.4660194174757, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=24.1176470588235, Blast_Score=139, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=24.7524752475248, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17560140, Length=506, Percent_Identity=22.9249011857708, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=521, Percent_Identity=25.1439539347409, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=22.8494623655914, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=27.2486772486773, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=401, Percent_Identity=24.4389027431421, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=26.8, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=25.7713248638838, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=27.0194986072423, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356441, Length=526, Percent_Identity=23.7642585551331, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648255, Length=513, Percent_Identity=22.8070175438596, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.0907297830375, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24653035, Length=516, Percent_Identity=22.6744186046512, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648260, Length=496, Percent_Identity=21.9758064516129, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=22.3076923076923, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=21.5094339622642, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648257, Length=543, Percent_Identity=22.8360957642726, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=19.8458574181118, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=22.3484848484848, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20130357, Length=521, Percent_Identity=20.9213051823417, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=20.7293666026871, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358303, Length=498, Percent_Identity=19.8795180722892, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60426; Mature: 60426

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
IENIQSQLATVQAYIIMTDKDELPNTTLEPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
IENIQSQLATVQAYIIMTDKDELPNTTLEPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQAEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]