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The map label for this gene is fruA

Identifier: 49478953

GI number: 49478953

Start: 3546715

End: 3548574

Strand: Reverse

Name: fruA

Synonym: BT9727_3462

Alternate gene names: 49478953

Gene position: 3548574-3546715 (Counterclockwise)

Preceding gene: 49478339

Following gene: 49478338

Centisome position: 67.75

GC content: 38.01

Gene sequence:

>1860_bases
ATGAAAATTACAGAACTATTAAAAAGGGATACAGTTATTATGAATTTGACAGCTTCAAATAAAGAAGCTGTCATAGATGA
ATTAGTTGAGAAATTAAACGGGGCAAATCGTTTAAATAGTAAAGCTGAATTTAAAGAAGCTATTTTAAAGCGGGAGTCAC
AAAGTACAACTGGCATTGGTGAAGGGATTGCTATACCTCATGCGAAAACAAAAGCTGTTAAGCAACCAGCGATTTGTTTT
GGTAGAAGTGTAAGCGGTATCAACTATGAATCGCTTGACGGACAGCCTGCGCATTTATTCTTTATGATTGCTGCGAGTGA
AGGGGCGAATAATACGCATTTAGAAACGTTATCACGCCTATCTACACTATTAATGGATGAAGGATTTCGTAAGCAATTGT
TAGAAGCAAAGGATGAAGAGGAACTTCTTCGTCTATTTGATGAAAAAGAAAATGAAAAAGAAGAAGAAGAGGTAGAAGTT
GCACAACCAGAAGGGAATGAACCGTACGTATTAGCTGTTACAGCTTGTCCAACTGGAATCGCTCACACATATATGGCTGC
GGATAGCTTGAAAGCAAAAGCAGCAGAGCTAGGAATTGCAATTAAAGTTGAAACGAATGGATCAACAGGTATAAAAAACG
GTTTAACGAAAGAGGATATTGAACGCGCAACAGCTATAATTGTTGCGGCAGATAAACAAGTAGAAATGAATCGTTTTGCT
GGTAAACATGTCATTCAAGTGCCAGTCGCTGACGGGATTAGAAAAACTGAAAAACTTCTTAATCGTGCTGTAAAACAGGA
TGCACCAATCTTTAAAGGAATAAAAGAAAATGGAAAGACAGAAAGTACAGAGAAAGAAAAAGGATTAGGAATTTATAAGC
ATTTAATGAACGGCGTAAGTAATATGCTTCCATTCGTTGTTGGTGGTGGGATTTTAATTGCGCTAGCGTTTTCGTTTGGT
GGTATAAAAGCAGAAGGTCCTTTAGCTGAATTGTTCATGTCTATTGGAGGAGGGGGAACAGGGGCATTTTTATTCCTTGT
ACCAATTTTAGCAGGATTTATTGCGAGTTCTATTGCTGATCGTCCTGGTTTTATGCCTGGTGTTGTCGGAGGATTTTTAG
CGGCACATGCGAATGCTGGCTTTTTAGGTGGATTAATTGCTGGTTTCTTAGCTGGATATGTTGTCTTAGGGCTAAAAAGA
CTATTTTCAGGATTACCAGTACAGTTAGAAGGTATTAAACCTGTTTTGTTATATCCAGTCTTTGGATTATTGATTACAGG
AGTTGTTATGCAAAAAGTAGTAATTCCGCCTGTAGTAGCATTGAATGAAATGTTAACAGGATGGTTAAATGGTTTGAACG
GTACAAATGCTATATTACTAGGTCTTATTTTAGGTGCGATGATGGCAATTGATATGGGTGGTCCAATTAATAAAGCAGCA
TTTACATTTGGTATTGCTGCAATAGAAGCACAGAACTTTGGAGTGCACTCAGCGGTTATGGCTGGTGGTATGGTACCGCC
GCTTGCAATTGCATTCGCAACTACATTCTTTAAATCGAAGTTTACAGAAGCGGAACGTAAGTCTGGTTTAACAAATTATA
TTATGGGAGCATCGTTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCAGATCCGGTTCGAGTAATTGTCAGTTGTGTT
GTCGGTTCAAGTATTGCGGGTGCATTATCTATGTTATTCCAAATTACATTACCGGCACCGCATGGTGGATTGTTTGTTAT
AGCATTAGTAAATAAACCAGTGTTATATATTTTCTCGATATTGATAGGGGCAGTTGTTTCTGCACTTATGATGGGGATTT
GGAAAAAGAAAGTTAAATAA

Upstream 100 bases:

>100_bases
GGAGTGCAACAGCATTTTCAGCTGATTTATGTGAAAAGGAAAAAGTAGAAGAATTATTGTCGCAAGTAATTGTAACTAAG
CGATAGGGGGGAAGCAGCAT

Downstream 100 bases:

>100_bases
TACGTGTAAAGGGAGTAGCTTTTATAAGTTGCTCCTTTTTATTACAAAAAAGGAGGTTAATAATTGGAGAATGATGATTT
TTGGTAAAAGATGTAATGAA

Product: PTS fructose-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MKITELLKRDTVIMNLTASNKEAVIDELVEKLNGANRLNSKAEFKEAILKRESQSTTGIGEGIAIPHAKTKAVKQPAICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDEEELLRLFDEKENEKEEEEVEV
AQPEGNEPYVLAVTACPTGIAHTYMAADSLKAKAAELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFA
GKHVIQVPVADGIRKTEKLLNRAVKQDAPIFKGIKENGKTESTEKEKGLGIYKHLMNGVSNMLPFVVGGGILIALAFSFG
GIKAEGPLAELFMSIGGGGTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAAHANAGFLGGLIAGFLAGYVVLGLKR
LFSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGAMMAIDMGGPINKAA
FTFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCV
VGSSIAGALSMLFQITLPAPHGGLFVIALVNKPVLYIFSILIGAVVSALMMGIWKKKVK

Sequences:

>Translated_619_residues
MKITELLKRDTVIMNLTASNKEAVIDELVEKLNGANRLNSKAEFKEAILKRESQSTTGIGEGIAIPHAKTKAVKQPAICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDEEELLRLFDEKENEKEEEEVEV
AQPEGNEPYVLAVTACPTGIAHTYMAADSLKAKAAELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFA
GKHVIQVPVADGIRKTEKLLNRAVKQDAPIFKGIKENGKTESTEKEKGLGIYKHLMNGVSNMLPFVVGGGILIALAFSFG
GIKAEGPLAELFMSIGGGGTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAAHANAGFLGGLIAGFLAGYVVLGLKR
LFSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGAMMAIDMGGPINKAA
FTFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCV
VGSSIAGALSMLFQITLPAPHGGLFVIALVNKPVLYIFSILIGAVVSALMMGIWKKKVK
>Mature_619_residues
MKITELLKRDTVIMNLTASNKEAVIDELVEKLNGANRLNSKAEFKEAILKRESQSTTGIGEGIAIPHAKTKAVKQPAICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDEEELLRLFDEKENEKEEEEVEV
AQPEGNEPYVLAVTACPTGIAHTYMAADSLKAKAAELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFA
GKHVIQVPVADGIRKTEKLLNRAVKQDAPIFKGIKENGKTESTEKEKGLGIYKHLMNGVSNMLPFVVGGGILIALAFSFG
GIKAEGPLAELFMSIGGGGTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAAHANAGFLGGLIAGFLAGYVVLGLKR
LFSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGAMMAIDMGGPINKAA
FTFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCV
VGSSIAGALSMLFQITLPAPHGGLFVIALVNKPVLYIFSILIGAVVSALMMGIWKKKVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=455, Percent_Identity=45.9340659340659, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1786951, Length=644, Percent_Identity=34.0062111801242, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI87082348, Length=437, Percent_Identity=33.6384439359268, Blast_Score=221, Evalue=2e-58,
Organism=Escherichia coli, GI1790386, Length=313, Percent_Identity=38.9776357827476, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1788729, Length=379, Percent_Identity=26.6490765171504, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=41.4893617021277, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789597, Length=156, Percent_Identity=29.4871794871795, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1788730, Length=86, Percent_Identity=43.0232558139535, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788726, Length=148, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367327, Length=142, Percent_Identity=22.5352112676056, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 65483; Mature: 65483

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITELLKRDTVIMNLTASNKEAVIDELVEKLNGANRLNSKAEFKEAILKRESQSTTGIG
CCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHCCCCCCCCCC
EGIAIPHAKTKAVKQPAICFGRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRL
CCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMDEGFRKQLLEAKDEEELLRLFDEKENEKEEEEVEVAQPEGNEPYVLAVTACPTGI
HHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHEECCCCCCCEEEEEECCCCCH
AHTYMAADSLKAKAAELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFA
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHC
GKHVIQVPVADGIRKTEKLLNRAVKQDAPIFKGIKENGKTESTEKEKGLGIYKHLMNGVS
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHH
NMLPFVVGGGILIALAFSFGGIKAEGPLAELFMSIGGGGTGAFLFLVPILAGFIASSIAD
HHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
RPGFMPGVVGGFLAAHANAGFLGGLIAGFLAGYVVLGLKRLFSGLPVQLEGIKPVLLYPV
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
FGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGAMMAIDMGGPINKAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
FTFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFI
HHHHHHEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TEGAIPFAAADPVRVIVSCVVGSSIAGALSMLFQITLPAPHGGLFVIALVNKPVLYIFSI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHH
LIGAVVSALMMGIWKKKVK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITELLKRDTVIMNLTASNKEAVIDELVEKLNGANRLNSKAEFKEAILKRESQSTTGIG
CCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHCCCCCCCCCC
EGIAIPHAKTKAVKQPAICFGRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRL
CCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMDEGFRKQLLEAKDEEELLRLFDEKENEKEEEEVEVAQPEGNEPYVLAVTACPTGI
HHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHEECCCCCCCEEEEEECCCCCH
AHTYMAADSLKAKAAELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFA
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHC
GKHVIQVPVADGIRKTEKLLNRAVKQDAPIFKGIKENGKTESTEKEKGLGIYKHLMNGVS
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHH
NMLPFVVGGGILIALAFSFGGIKAEGPLAELFMSIGGGGTGAFLFLVPILAGFIASSIAD
HHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
RPGFMPGVVGGFLAAHANAGFLGGLIAGFLAGYVVLGLKRLFSGLPVQLEGIKPVLLYPV
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
FGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGAMMAIDMGGPINKAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
FTFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFI
HHHHHHEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TEGAIPFAAADPVRVIVSCVVGSSIAGALSMLFQITLPAPHGGLFVIALVNKPVLYIFSI
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHH
LIGAVVSALMMGIWKKKVK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]