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The map label for this gene is recG

Identifier: 49478911

GI number: 49478911

Start: 3691315

End: 3693288

Strand: Reverse

Name: recG

Synonym: BT9727_3596

Alternate gene names: 49478911

Gene position: 3693288-3691315 (Counterclockwise)

Preceding gene: 49479406

Following gene: 49478418

Centisome position: 70.51

GC content: 39.46

Gene sequence:

>1974_bases
ATGGGAATTTATACAGTTTCTCATCTATTAGAACATTTTCCGTACCGTTATGAAGACTATGCGATGAAAGATCTTGCTGA
AGTAAAGCATGATGAACGTGTAACAGTAGAGGGGAAAGTTCATAGTGCTCCTTTACTGCAATATTATGGGAAGAAGAAGT
CGCGTCTTACGGTTCGTGTCCTCGTAGGTCGTTATTTAATTACAGCTGTATGTTTTAATAGGCCGTACTATAAGCAAAAG
TTAAATTTAGATGAAACGGTAACGATTACTGGTAAATGGGATCAGCATCGTCAAACGATTGCGGTATCAGAACTTCATTT
TGGACCGGTTGTACGTCAACAAGAAGTAGAGCCTGTGTACTCAGTGAAAGGGAAACTTACAGTAAAACAGATGCGCCGTT
TTATTGCACAAGCGTTAAAAGAGTATGGAGATTCTATAGTTGAAGTGTTACCTGATGGATTGCTAAGTCGATATAAATTA
TTGCCACGTTATGAAGCGCTTCGGGCGTTGCATTTTCCAACAGGTCAGGAAGATTTGAAGCAAGCGCGTCGCCGTTTTGT
ATATGAGGAGTTTTTCTTATTTCAGTTAAAAATGCAAACGTTACGGAAAATGGAGAGGGAAAATTCGAAAGGGACGAAAA
AAGAAATTCCTTCAGAAGAATTGCAAGAGTTTATCGATGCTCTTCCGTTTCCGTTAACTGGTGCGCAACGCCGAGTTGTA
GATGAAATTATGAAAGATATGACATCTCCTTATCGAATGAATCGTCTGTTGCAAGGTGATGTAGGTTCTGGTAAAACGGT
GGTTGCTGCAATTGGTCTTTATGCAGCAAAATTAGCACATTATCAAGGTGCTTTAATGGTTCCAACAGAAATTTTAGCAG
AACAACATTATCAATCGCTCGCAGAGACGTTCTCGCACTTTGGTATGAAGGTTGAATTGTTAACAAGTTCAGTTAAAGGT
GTGAGACGCCGAGAGATTTTGGCAAAATTAGAACAAGGAGAAATAGATATCCTTGTTGGAACACATGCTTTAATTCAAGA
TGAAGTGATCTTTCATAGGTTAGGTCTCGTTATTACTGATGAACAACATCGATTTGGTGTAGCGCAGCGACGAGTTTTAC
GTGAGAAAGGTGAAAGTCCAGACGTATTATTTATGACAGCGACTCCGATTCCACGTACGTTAGCAATTACTGCATTTGGA
GAGATGGATGTTTCTATAATCGATGAGATGCCAGCTGGTAGAAAGGTAATTGAAACATACTGGGCAAAACATGACATGTT
AGATCGTGTTCTCGGTTTTGTAGAGAAAGAGATAAACAAAGGAAGACAAGCATATGTTATTTGTCCCCTTATTGAAGAAT
CTGAGAAACTGGATGTACAAAATGCAATCGACTTACATAGTATGCTGACTCATCATTATCAGGGTAAATGTCAAGTTGGA
TTAATGCATGGAAGATTATCATCTCAAGAAAAAGAAGAGATAATGGGACAATTTAGTGAAAATAAAGTGCAAATTCTTGT
ATCGACAACAGTTGTTGAAGTAGGTGTGAACGTACCGAATGCGACTGTAATGGTTATTTATGATGCAGAGCGTTTCGGTT
TATCGCAGCTTCATCAGCTAAGAGGACGTGTTGGGCGTGGTAGTGAACAATCGTATTGTTTATTAATCGCGGATCCAAAA
TCGGAAACGGGAAAAGAACGAATGCGTATTATGACCGAAACGAATGATGGATTTGTATTGTCGGAAAAAGATTTAGAGTT
AAGAGGTCCTGGAGATTTCTTTGGGAGTAAGCAAAGTGGTTTACCAGAATTCAAGGTTGCTGATATGGTACATGATTATC
GGGCGTTAGAAACAGCTAGACAAGATGCGGCGTTACTAGTAGATTCAGAAGCATTTTGGCATAATGATCAATATGCGTCT
TTGCGTACGTATCTTGACGGGACAGGTGTGTTCCAGGGAGAGAAGCTCGATTAA

Upstream 100 bases:

>100_bases
AATAATGTGACGGAGCGTGAGAATCTTGAATGAAGTTGTACAAGTTCCCGTTACGGATGTAAAGGGAATCGGAGGAGAAA
CATCTGAATTACTACACGAG

Downstream 100 bases:

>100_bases
AGGGAGGCGACAAAAGTTTTTGTTGCATTCTACTTTTAATGATTATATACTACTGTTAGTACCTAGTCATAAAAATGGAT
GGTGCGGTATGAAAAAAAGA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MGIYTVSHLLEHFPYRYEDYAMKDLAEVKHDERVTVEGKVHSAPLLQYYGKKKSRLTVRVLVGRYLITAVCFNRPYYKQK
LNLDETVTITGKWDQHRQTIAVSELHFGPVVRQQEVEPVYSVKGKLTVKQMRRFIAQALKEYGDSIVEVLPDGLLSRYKL
LPRYEALRALHFPTGQEDLKQARRRFVYEEFFLFQLKMQTLRKMERENSKGTKKEIPSEELQEFIDALPFPLTGAQRRVV
DEIMKDMTSPYRMNRLLQGDVGSGKTVVAAIGLYAAKLAHYQGALMVPTEILAEQHYQSLAETFSHFGMKVELLTSSVKG
VRRREILAKLEQGEIDILVGTHALIQDEVIFHRLGLVITDEQHRFGVAQRRVLREKGESPDVLFMTATPIPRTLAITAFG
EMDVSIIDEMPAGRKVIETYWAKHDMLDRVLGFVEKEINKGRQAYVICPLIEESEKLDVQNAIDLHSMLTHHYQGKCQVG
LMHGRLSSQEKEEIMGQFSENKVQILVSTTVVEVGVNVPNATVMVIYDAERFGLSQLHQLRGRVGRGSEQSYCLLIADPK
SETGKERMRIMTETNDGFVLSEKDLELRGPGDFFGSKQSGLPEFKVADMVHDYRALETARQDAALLVDSEAFWHNDQYAS
LRTYLDGTGVFQGEKLD

Sequences:

>Translated_657_residues
MGIYTVSHLLEHFPYRYEDYAMKDLAEVKHDERVTVEGKVHSAPLLQYYGKKKSRLTVRVLVGRYLITAVCFNRPYYKQK
LNLDETVTITGKWDQHRQTIAVSELHFGPVVRQQEVEPVYSVKGKLTVKQMRRFIAQALKEYGDSIVEVLPDGLLSRYKL
LPRYEALRALHFPTGQEDLKQARRRFVYEEFFLFQLKMQTLRKMERENSKGTKKEIPSEELQEFIDALPFPLTGAQRRVV
DEIMKDMTSPYRMNRLLQGDVGSGKTVVAAIGLYAAKLAHYQGALMVPTEILAEQHYQSLAETFSHFGMKVELLTSSVKG
VRRREILAKLEQGEIDILVGTHALIQDEVIFHRLGLVITDEQHRFGVAQRRVLREKGESPDVLFMTATPIPRTLAITAFG
EMDVSIIDEMPAGRKVIETYWAKHDMLDRVLGFVEKEINKGRQAYVICPLIEESEKLDVQNAIDLHSMLTHHYQGKCQVG
LMHGRLSSQEKEEIMGQFSENKVQILVSTTVVEVGVNVPNATVMVIYDAERFGLSQLHQLRGRVGRGSEQSYCLLIADPK
SETGKERMRIMTETNDGFVLSEKDLELRGPGDFFGSKQSGLPEFKVADMVHDYRALETARQDAALLVDSEAFWHNDQYAS
LRTYLDGTGVFQGEKLD
>Mature_656_residues
GIYTVSHLLEHFPYRYEDYAMKDLAEVKHDERVTVEGKVHSAPLLQYYGKKKSRLTVRVLVGRYLITAVCFNRPYYKQKL
NLDETVTITGKWDQHRQTIAVSELHFGPVVRQQEVEPVYSVKGKLTVKQMRRFIAQALKEYGDSIVEVLPDGLLSRYKLL
PRYEALRALHFPTGQEDLKQARRRFVYEEFFLFQLKMQTLRKMERENSKGTKKEIPSEELQEFIDALPFPLTGAQRRVVD
EIMKDMTSPYRMNRLLQGDVGSGKTVVAAIGLYAAKLAHYQGALMVPTEILAEQHYQSLAETFSHFGMKVELLTSSVKGV
RRREILAKLEQGEIDILVGTHALIQDEVIFHRLGLVITDEQHRFGVAQRRVLREKGESPDVLFMTATPIPRTLAITAFGE
MDVSIIDEMPAGRKVIETYWAKHDMLDRVLGFVEKEINKGRQAYVICPLIEESEKLDVQNAIDLHSMLTHHYQGKCQVGL
MHGRLSSQEKEEIMGQFSENKVQILVSTTVVEVGVNVPNATVMVIYDAERFGLSQLHQLRGRVGRGSEQSYCLLIADPKS
ETGKERMRIMTETNDGFVLSEKDLELRGPGDFFGSKQSGLPEFKVADMVHDYRALETARQDAALLVDSEAFWHNDQYASL
RTYLDGTGVFQGEKLD

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=646, Percent_Identity=40.0928792569659, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787357, Length=388, Percent_Identity=37.6288659793814, Blast_Score=268, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75014; Mature: 74883

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIYTVSHLLEHFPYRYEDYAMKDLAEVKHDERVTVEGKVHSAPLLQYYGKKKSRLTVRV
CCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCEEHHH
LVGRYLITAVCFNRPYYKQKLNLDETVTITGKWDQHRQTIAVSELHFGPVVRQQEVEPVY
HHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEHHCCCCCCCHHCCCHHH
SVKGKLTVKQMRRFIAQALKEYGDSIVEVLPDGLLSRYKLLPRYEALRALHFPTGQEDLK
HCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHH
QARRRFVYEEFFLFQLKMQTLRKMERENSKGTKKEIPSEELQEFIDALPFPLTGAQRRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHH
DEIMKDMTSPYRMNRLLQGDVGSGKTVVAAIGLYAAKLAHYQGALMVPTEILAEQHYQSL
HHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHH
AETFSHFGMKVELLTSSVKGVRRREILAKLEQGEIDILVGTHALIQDEVIFHRLGLVITD
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCEEEEC
EQHRFGVAQRRVLREKGESPDVLFMTATPIPRTLAITAFGEMDVSIIDEMPAGRKVIETY
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHH
WAKHDMLDRVLGFVEKEINKGRQAYVICPLIEESEKLDVQNAIDLHSMLTHHYQGKCQVG
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
LMHGRLSSQEKEEIMGQFSENKVQILVSTTVVEVGVNVPNATVMVIYDAERFGLSQLHQL
EEECCCCCHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCHHCCHHHHHHH
RGRVGRGSEQSYCLLIADPKSETGKERMRIMTETNDGFVLSEKDLELRGPGDFFGSKQSG
HHCCCCCCCCCEEEEEECCCCCCCHHHHEEEEECCCCEEEECCCCEECCCCCCCCCCCCC
LPEFKVADMVHDYRALETARQDAALLVDSEAFWHNDQYASLRTYLDGTGVFQGEKLD
CCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GIYTVSHLLEHFPYRYEDYAMKDLAEVKHDERVTVEGKVHSAPLLQYYGKKKSRLTVRV
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCEEHHH
LVGRYLITAVCFNRPYYKQKLNLDETVTITGKWDQHRQTIAVSELHFGPVVRQQEVEPVY
HHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEHHCCCCCCCHHCCCHHH
SVKGKLTVKQMRRFIAQALKEYGDSIVEVLPDGLLSRYKLLPRYEALRALHFPTGQEDLK
HCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHH
QARRRFVYEEFFLFQLKMQTLRKMERENSKGTKKEIPSEELQEFIDALPFPLTGAQRRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHH
DEIMKDMTSPYRMNRLLQGDVGSGKTVVAAIGLYAAKLAHYQGALMVPTEILAEQHYQSL
HHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHH
AETFSHFGMKVELLTSSVKGVRRREILAKLEQGEIDILVGTHALIQDEVIFHRLGLVITD
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCEEEEC
EQHRFGVAQRRVLREKGESPDVLFMTATPIPRTLAITAFGEMDVSIIDEMPAGRKVIETY
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHH
WAKHDMLDRVLGFVEKEINKGRQAYVICPLIEESEKLDVQNAIDLHSMLTHHYQGKCQVG
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
LMHGRLSSQEKEEIMGQFSENKVQILVSTTVVEVGVNVPNATVMVIYDAERFGLSQLHQL
EEECCCCCHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCHHCCHHHHHHH
RGRVGRGSEQSYCLLIADPKSETGKERMRIMTETNDGFVLSEKDLELRGPGDFFGSKQSG
HHCCCCCCCCCEEEEEECCCCCCCHHHHEEEEECCCCEEEECCCCEECCCCCCCCCCCCC
LPEFKVADMVHDYRALETARQDAALLVDSEAFWHNDQYASLRTYLDGTGVFQGEKLD
CCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]