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The map label for this gene is priA

Identifier: 49478905

GI number: 49478905

Start: 3705691

End: 3708096

Strand: Reverse

Name: priA

Synonym: BT9727_3609

Alternate gene names: 49478905

Gene position: 3708096-3705691 (Counterclockwise)

Preceding gene: 49478424

Following gene: 49478423

Centisome position: 70.8

GC content: 37.49

Gene sequence:

>2406_bases
ATGAAATTTGCAAGTGTAATTGTTGATGTACCTGCACGTCAGACAGATCGACCATTTGATTATATTATCCCTAAAAAATG
GGAAGATATTGTCCAGACAGGCATGCGTGTAGTAGTTCCGTTTGGCCCAAGGAAATTGCAAGGTTTTATTATTGGAATTA
AAAATTCGGTTGAAGTAGAAAGTAAAAAGTTAAAGACAATCCACGAAATTTTAGATGTAACACCGGTTTTAAATGAAGAG
TTGTTAAAGCTTGGGTATTGGCTTACAAGTGAAACGTTATGTTATATGATTTCAGCTTTTCAAGTTATGCTTCCGACGGC
GATAAAAGCAACATATAAAAAACGTCTACAGCTTCGTAAACAAGAAGAAGTAGCACCTGAACTACTGTTTTTATTTCAGG
AAAAAGAAGCGATAGATTGGGAAGCGATTGAGACGCAACCACATCTATACCGCACGATTCAACAAGAAATTAAACATGGC
ACGATTGAAGTCGTTTATCAAGTAAAAGATAAAGTACAAAAGAAGAAACAAAGAGTCGTTCAACCGGAGTTGCCAGAAGA
TAAATTAGAATTGGCAGCATTTGAACTGAAAAGTAAAAAACAACAAGATGTCCTCTATTATTTTGTGGAAAATTATAAAA
GTGTGCCGTTGAAGGTAATAACAGAAGAGTTGCAAATAACAGATGCTCCGATTAAAGCACTTGTTAAAAAGGGGCTAATC
TCAGAAAAGTATGTGGAAGTATATCGAAATCCGTATGACGATGACGATTTTGAACAAACGAAACCATTCCCACTTACTGA
GGAACAAAAGCAAGTTATTACACCGATTTTATCATCAATTACAAATGAAACTTACAATCCATTTTTACTATATGGTGTTA
CAGGAAGCGGAAAAACAGAAGTATATTTACAATCTATAGCAGCGGTGCTCGAGAAAGGAAAAGAAGCTATTGTACTTGTT
CCTGAAATTGCACTAACGCCTCAGATGGTAGATCGTTTTAAAGGTAGATTTGGCTCACAAGTTGCGGTTCTTCATAGTGC
GTTGTCTGTTGGAGAAAAATATGATGAATGGCGTAAGATTTTAAGAAAAGAAGTGAAGGTTGTAGTTGGTGCACGTTCAG
CTGTATTTGCTCCTTTTGAAAATTTAGGGATTATTATTATTGATGAGGAACATGAATCGAGTTATAAGCAAGAAGATAAT
CCGAGGTATCATGCAAGGGATGTAGCTGTGTGGAGAGGACAATATCATAAATGTCCTATCGTTCTTGGTAGTGCAACACC
GACGCTTGAATCATTTGCAAGAGCGAAAAAAGGTGTGTATGAATTACTGACGATGGAAAAACGTATGAACGAACAAGCTT
TACCGACAGTAGAGATTGTTGATATGCGTGAGGAACTTCGTGACGGGAATCGCTCCATGTTTTCAAAGGCACTGCATGAA
AAAATAGCAGATCGATTAGAAAAGAAAGAACAAATGGTGCTCTTTTTAAATAGAAGGGGGCATTCTACATTTGTTATGTG
CCGTGATTGCGGGTACGTTGTACAATGTCCGCATTGTGACATCTCACTAACATATCATAAAATGAACCATCGTTTAAAAT
GCCATTATTGCAGTTACGAAGAAAATATGCCGACTGCGTGTCCTGCTTGTCAAAGTACATACATTCGTTTCTTTGGTACG
GGTACGCAAAAGGTAGAAGAAGAAATTACAAAACTATTTCCAGAGGCACGAGTCATTCGAATGGATGTAGATACGACGAG
TCGTAAAGGGATGCATGAGAAATTATTAAAGGCGTTCGGGGAAGAAAAAGCAGATATATTACTTGGAACACAAATGATTG
CGAAGGGGTTAGATTTTCCGAAAGTAACGCTTGTTGGGGTTTTAACTGCTGATACGATGCTTCATTTACCTGATTTTAGG
GCGAGTGAAAAGACTTATCAGTTATTGACGCAAGTAAGTGGACGAGCAGGCCGACATGAATTGCCAGGGGAAGTTATAAT
TCAAACGTATACACCAGAACATTACAGTATAGAGTTAGCAAAGAACCAACAATACGATGTGTTTTTTGACCAAGAAATGC
AAATGAGACGAACGAGACAATATCCACCTTATTACTATGTTGTACTTGTGACGGTATCTCACCCGGAATTATTGAAGGCA
GTACAAGTGACGGAAAAAATTGTCGGTCATTTACGATCGCACTGCTCGCAGCAAACAATGGTGTTAGGACCGGTTGCTTC
AGCAATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGAACGTGC
TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAAGAGCTACAAATCTCAATTGATTTTAATCCAACAATGTTA
ATGTAG

Upstream 100 bases:

>100_bases
GAAAAGATGGAGAAGTTATAGAGTTGCCACTTTTAACGAAGAAAGAGGTTGCTCGTGAAATATTAAAGCAAATTGAAATG
ATGTTAGAAGATGATCGTCT

Downstream 100 bases:

>100_bases
CGGAGGGAAATATGGCAGTTTTAGAAATAGTAAAGCATCCAAATGAAGTGTTAGAAACACCTTGTGAAAGAGTAATTAAC
TTTGATAAAAAGTTAGTGAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG
TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M

Sequences:

>Translated_801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG
TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M
>Mature_801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG
TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=556, Percent_Identity=39.568345323741, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787357, Length=191, Percent_Identity=25.130890052356, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 92626; Mature: 92626

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE
CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH
SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV
HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC
QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI
HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC
ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKELQISIDFNPTMLM
HHHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE
CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH
SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV
HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC
QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI
HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC
ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKELQISIDFNPTMLM
HHHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]