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The map label for this gene is pgk

Identifier: 49478894

GI number: 49478894

Start: 4867870

End: 4869054

Strand: Reverse

Name: pgk

Synonym: BT9727_4817

Alternate gene names: 49478894

Gene position: 4869054-4867870 (Counterclockwise)

Preceding gene: 49479301

Following gene: 49478893

Centisome position: 92.96

GC content: 41.27

Gene sequence:

>1185_bases
ATGAACAAAAAATCAATTCGTGACGTAGATTTAAAAGGTAAGCGTGTATTTTGCCGCGTTGACTTCAACGTACCTATGAA
AGAAGGCAAAATTACAGATGAAACTCGTATCCGTGCAGCTCTTCCTACAATTCAATATTTAGTAGAGCAAGGTGCGAAGG
TTATTTTAGCAAGCCACTTAGGCCGTCCAAAAGGTCAAGCAGTAGAAGAATTACGTCTTACTCCAGTAGCAGCACGTTTA
GGCGAGCTTCTTGGTAAAGATGTTAAAAAAGCAGACGAAGCATTCGGACCAGTTGCACAAGAAATGGTTGCAGCAATGAA
CGAAGGCGACGTATTAGTTCTTGAAAACGTACGTTTCTATGCGGGCGAAGAAAAGAACGATGCAGAACTTGCGAAAGAAT
TTGCAGCTCTTGCTGACATCTTCGTAAACGATGCATTCGGTGCAGCTCACCGTGCTCATGCTTCTACAGCAGGAATCGCA
GACTACCTACCAGCAGTATCTGGTCTATTAATGGAAAAAGAGTTAGAAGTATTAGGTAAAGCACTTTCTAACCCAGAACG
TCCATTCACAGCTATCATCGGTGGTGCGAAAGTAAAAGATAAAATCGGTGTAATTCGTCATCTATTAGACAAAGTAGATA
ACTTAATCATCGGTGGCGGACTTGCTTACACATTCGTTAAAGCTTTAGGTCATGAAATTGGTCTATCTCTATGTGAAGAC
GACAAGATTGAACTAGCTAAAGAATTTATGCAACTTGCAAAAGAAAAAGGCGTAAACTTCTACATGCCAGTTGATGTTGT
AATCACTGAGGAATTCTCTGAAACTGCAACAACTAAAATCGTTGGTATCGACTCTATCCCTTCTAACTGGGAAGGCGTGG
ACATCGGTCCTAAAACACGTGAAATTTATGCAGATGTAATTAAAAACTCTAAGCTTGTTGTATGGAATGGACCAATGGGT
GTATTCGAAATGACTCCATTCTCACAAGGTACAAAAGCAGTAGGACAAGCATTAGCAGATGCAGAAGGTACATACTCTGT
TATCGGCGGTGGTGACTCTGCAGCAGCTGTTGAAAAATTCGGTATGGCTGATAAAATGAGCCACATTTCTACTGGCGGCG
GTGCGTCATTAGAATTCATGGAAGGTAAAGAGCTTCCAGGTGTAGTTTGTCTTAACGACAAATAA

Upstream 100 bases:

>100_bases
AGGGTTTGTTCCCTCTCTCTCTTCTTTCGTTTTAAAAGCAAATGTGTTAAGCTTTTGAGTGGGTTTTGTCAATCCTAACT
AGTAGTCGGAGGGAAATCCA

Downstream 100 bases:

>100_bases
GTAGCAGCCAAAGAAAAAGGACGGTGCAAAGCATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCTA
TCTGAAGCAGTTAGCTTCGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSQGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Sequences:

>Translated_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSQGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
>Mature_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFSQGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=46.8599033816425, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.2469733656174, Blast_Score=344, Evalue=7e-95,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=46.7171717171717, Blast_Score=347, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=47.8155339805825, Blast_Score=363, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=49.0291262135922, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=46.9586374695864, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=45.5421686746988, Blast_Score=332, Evalue=3e-91,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42299; Mature: 42299

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQAVEELRLTPVAARLGELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFY
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFSQGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC
>Mature Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQAVEELRLTPVAARLGELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFY
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHCCHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFSQGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA