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The map label for this gene is moaE

Identifier: 49478737

GI number: 49478737

Start: 4507859

End: 4508323

Strand: Reverse

Name: moaE

Synonym: BT9727_4452

Alternate gene names: 49478737

Gene position: 4508323-4507859 (Counterclockwise)

Preceding gene: 49481544

Following gene: 49478610

Centisome position: 86.07

GC content: 40.43

Gene sequence:

>465_bases
ATGACACATACGTATTATGAAGTAATTGATACAGAAATTTCAATTGAAGAGGTAGCGAATAAGGTTATTCGCCGCGAATG
TGGTGCTGTTACAACATTTATTGGAACGGTTAGAGAGTTTACGAAAGGGCGTCGTACATTATACTTAGAATATGTCGCTT
ATAAAACGATGGCAGAAAAGATGCTAGAGAAAATCGGATCAGAAGTGAAAAAGAAATGGCCAGGTACACACGTTGCGATT
ACACATCGCATCGGTACACTGCAAATTTCTGATATTGCGGTTGTTGTGGCTGTTTCAACGCCGCATCGTAAAGCGGCTTA
TGAAGCAAATGAATATATTATGGAACGCATAAAACAAATTGTTCCGATTTGGAAAAAGGAGTTTTGGGAAGATGGAGACT
CTTGGATTGGCGATCAATTAGAAAAAACGCCATATCCGGCGGGAGAGCCTGGGAAGGAGCTATAA

Upstream 100 bases:

>100_bases
ATGATGCTCCTGCAAACTTACGAGAAGAATATAAAGTATTTCATATAACAGAAGAAGAGTTGTATATAGACTGGTTTTTA
CAAACGGTAAGGAGTGCGAG

Downstream 100 bases:

>100_bases
GTATGATTCGAGTATTGTTATTTGCGAACTTGCAAGAAGAAGCAGGAAGAAGTGAATTACAAATAGAGAAAGAAAATATT
ACGGTTGCAGAGTTAAAAGA

Product: molybdopterin converting factor, large subunit (subunit 2)

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTHTYYEVIDTEISIEEVANKVIRRECGAVTTFIGTVREFTKGRRTLYLEYVAYKTMAEKMLEKIGSEVKKKWPGTHVAI
THRIGTLQISDIAVVVAVSTPHRKAAYEANEYIMERIKQIVPIWKKEFWEDGDSWIGDQLEKTPYPAGEPGKEL

Sequences:

>Translated_154_residues
MTHTYYEVIDTEISIEEVANKVIRRECGAVTTFIGTVREFTKGRRTLYLEYVAYKTMAEKMLEKIGSEVKKKWPGTHVAI
THRIGTLQISDIAVVVAVSTPHRKAAYEANEYIMERIKQIVPIWKKEFWEDGDSWIGDQLEKTPYPAGEPGKEL
>Mature_153_residues
THTYYEVIDTEISIEEVANKVIRRECGAVTTFIGTVREFTKGRRTLYLEYVAYKTMAEKMLEKIGSEVKKKWPGTHVAIT
HRIGTLQISDIAVVVAVSTPHRKAAYEANEYIMERIKQIVPIWKKEFWEDGDSWIGDQLEKTPYPAGEPGKEL

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=128, Percent_Identity=41.40625, Blast_Score=125, Evalue=2e-29,
Organism=Escherichia coli, GI1787003, Length=113, Percent_Identity=40.7079646017699, Blast_Score=90, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509279, Length=128, Percent_Identity=35.15625, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356259, Length=131, Percent_Identity=37.4045801526718, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 17694; Mature: 17562

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTYYEVIDTEISIEEVANKVIRRECGAVTTFIGTVREFTKGRRTLYLEYVAYKTMAEK
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
MLEKIGSEVKKKWPGTHVAITHRIGTLQISDIAVVVAVSTPHRKAAYEANEYIMERIKQI
HHHHHHHHHHHHCCCCEEEEEECCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
VPIWKKEFWEDGDSWIGDQLEKTPYPAGEPGKEL
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
THTYYEVIDTEISIEEVANKVIRRECGAVTTFIGTVREFTKGRRTLYLEYVAYKTMAEK
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
MLEKIGSEVKKKWPGTHVAITHRIGTLQISDIAVVVAVSTPHRKAAYEANEYIMERIKQI
HHHHHHHHHHHHCCCCEEEEEECCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
VPIWKKEFWEDGDSWIGDQLEKTPYPAGEPGKEL
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]