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The map label for this gene is ytzG [H]

Identifier: 49478734

GI number: 49478734

Start: 4497053

End: 4497781

Strand: Reverse

Name: ytzG [H]

Synonym: BT9727_4438

Alternate gene names: 49478734

Gene position: 4497781-4497053 (Counterclockwise)

Preceding gene: 49481727

Following gene: 49478733

Centisome position: 85.87

GC content: 35.12

Gene sequence:

>729_bases
GTGAGATTAGATAAATTATTAGCAAATATGGGATATGGAAGTAGAAAAGAAGTAAAGAAATTATTGAAAGACGGCGTTGT
GAAAATTGATGGAATATCGGTGAAAGATGCAAAGGTTCATGTAAATGTAGAAGAACAAGAAGTTATGATTCACGGTGAAG
TCGTGGAATATAAAGAGTTTGTCTACCTAATGATGCATAAACCACAAGGCGTTATTTCAGCGACAGAAGATGATAATCAT
GAAACGGTAATAGATTTATTAGAGTTAGAAGATGCGATTTTTAATCCGTTCCCAGTTGGAAGACTTGATATTGATACAGA
AGGTTTCTTATTAATAACAAATGACGGGAAGTTATCACATCAATTGTTATCTCCGAAAAAGCATGTGCCGAAAAAATATT
ATGCACACGTTGCAGGAGTAGTAACAGAAGAGGATGTAAAAGAGTTTGCTAAAGGTGTTATTTTAGATGATGGCTATGAG
ACAAAGCCAGGTGCACTTACAATATTAAAAAGCGATGATATTTCTGAAATCGAGCTTGTCATTACAGAAGGAAAGTTCCA
TCAAGTGAAGCGTATGTTTGAAGCGGTAGGGAAAAAAGTAGTCTACTTAAAGAGAACAGAGATGGGACCGTTAGTGTTAG
ATGAAGAGCTAGAACTTGGACAATACCGTGAGTTAACAGATGAAGAAGTAGAGATGTTAAAAACATATCAAGTAGATACT
GAGAAATAG

Upstream 100 bases:

>100_bases
AGATCCAACGTAAACTTGGCAATAGATTCAAAATAAAGTTGAAATCAAAAGGGGCGTAATTACGTCCCCTTTTCTATGAT
AAATAAAGAAGGTGAAAAAC

Downstream 100 bases:

>100_bases
TGCTATTTTTAGGCGCAGAAAAGACCCCTTTCTAATGAGTTAGAAAGGGGTATGTATTTATTTACATACAATTATTTTTC
GTTATGAGGTCATTCTCACT

Product: pseudouridylate synthase (ribosomal small subunit pseudouridine synthase A)

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQVDT
EK

Sequences:

>Translated_242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQVDT
EK
>Mature_242_residues
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEFVYLMMHKPQGVISATEDDNH
ETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSHQLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYE
TKPGALTILKSDDISEIELVITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQVDT
EK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=37.9310344827586, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=32.3886639676113, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=29.7872340425532, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=30.939226519337, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27505; Mature: 27505

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCEEECCEEEEEECHHEEEEECHHHHHHHE
VYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSH
EEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEIELV
HHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEEEEE
ITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQVDT
EECCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCCHHCCCHHHHHHHHHHCCCC
EK
CC
>Mature Secondary Structure
MRLDKLLANMGYGSRKEVKKLLKDGVVKIDGISVKDAKVHVNVEEQEVMIHGEVVEYKEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCEEECCEEEEEECHHEEEEECHHHHHHHE
VYLMMHKPQGVISATEDDNHETVIDLLELEDAIFNPFPVGRLDIDTEGFLLITNDGKLSH
EEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
QLLSPKKHVPKKYYAHVAGVVTEEDVKEFAKGVILDDGYETKPGALTILKSDDISEIELV
HHCCCHHHCCHHHHHHHHHCCCHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCEEEEE
ITEGKFHQVKRMFEAVGKKVVYLKRTEMGPLVLDEELELGQYRELTDEEVEMLKTYQVDT
EECCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCCHHCCCHHHHHHHHHHCCCC
EK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]