Click here to switch to the map view.

The map label for this gene is gntR

Identifier: 49478698

GI number: 49478698

Start: 4401977

End: 4402621

Strand: Reverse

Name: gntR

Synonym: BT9727_4331

Alternate gene names: 49478698

Gene position: 4402621-4401977 (Counterclockwise)

Preceding gene: 49481335

Following gene: 49476883

Centisome position: 84.06

GC content: 34.11

Gene sequence:

>645_bases
TTGACATCATCAAATACAAAAGTATATCTCGAAATTGTAAAAAAAATCCGTTCCATTATGGAAGAGGATGGATTGGTAGC
AGGGGATCGTTTACCGTCTGAGCGTGAGTTAAGTTCACGTTTAAACGTAGGACGTTCCTCTGTAAGAGAAGCGTTGCGTG
CTTTAGAACTTGTAGGGTTAATTGAAACGAGACGTGGTGAAGGGACGTTTATTCGAAACTTTTACGATAACGGTCTTGTA
CAATTGATTGCTCCGTTCTTGCTGCAAGATGAGAAAACAATTCGTGATTTATTACAAACAAAACGATTGCTTGAGAAAGA
TATGATTCGAATTGTATGTAATTTACCGAAAGAAACGTTTTCTAAAGTGCTAAGTAAATTACATCAAGTGCTTGAAGGAA
ATGAAAACTCAATTTCAATGCTTCACCAAACGTTCTTTAAAACTCTTATTGAACAAGTCGATAATTATTTACTATATCGC
ATTTGGATGATCGTAAATGATTATGTATCAACTCTTTCTTGTAAAGTTTCAGGAGATTCTATCGATATGTACAGAAAGCT
TTATGCTACTTTGGAAGAAAAACAAGAAAATGATGCACTAAAAATTTACGATGAATTAGTAGAGAATATACAGTTTCACT
CGTAA

Upstream 100 bases:

>100_bases
TGGAGACAGGAGTAGCTCGCCGTCAAGTGGATCCAAATGAAGTTGCTGAGAAAACAAAACAATTAGCGCTGATTGGTAAA
GAATAAGCGAGGAATTTCCA

Downstream 100 bases:

>100_bases
TGTATAAAATTTGTCGAAATGACCATGACACGTTATGACAAACATTCTATCGCGTAGCGGTTAAAGTTAAACGTAAAGAG
TGAGGTTATTAGACAGATTG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSSNTKVYLEIVKKIRSIMEEDGLVAGDRLPSERELSSRLNVGRSSVREALRALELVGLIETRRGEGTFIRNFYDNGLV
QLIAPFLLQDEKTIRDLLQTKRLLEKDMIRIVCNLPKETFSKVLSKLHQVLEGNENSISMLHQTFFKTLIEQVDNYLLYR
IWMIVNDYVSTLSCKVSGDSIDMYRKLYATLEEKQENDALKIYDELVENIQFHS

Sequences:

>Translated_214_residues
MTSSNTKVYLEIVKKIRSIMEEDGLVAGDRLPSERELSSRLNVGRSSVREALRALELVGLIETRRGEGTFIRNFYDNGLV
QLIAPFLLQDEKTIRDLLQTKRLLEKDMIRIVCNLPKETFSKVLSKLHQVLEGNENSISMLHQTFFKTLIEQVDNYLLYR
IWMIVNDYVSTLSCKVSGDSIDMYRKLYATLEEKQENDALKIYDELVENIQFHS
>Mature_213_residues
TSSNTKVYLEIVKKIRSIMEEDGLVAGDRLPSERELSSRLNVGRSSVREALRALELVGLIETRRGEGTFIRNFYDNGLVQ
LIAPFLLQDEKTIRDLLQTKRLLEKDMIRIVCNLPKETFSKVLSKLHQVLEGNENSISMLHQTFFKTLIEQVDNYLLYRI
WMIVNDYVSTLSCKVSGDSIDMYRKLYATLEEKQENDALKIYDELVENIQFHS

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=99, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24842; Mature: 24711

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNTKVYLEIVKKIRSIMEEDGLVAGDRLPSERELSSRLNVGRSSVREALRALELVGL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
IETRRGEGTFIRNFYDNGLVQLIAPFLLQDEKTIRDLLQTKRLLEKDMIRIVCNLPKETF
HHHCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKVLSKLHQVLEGNENSISMLHQTFFKTLIEQVDNYLLYRIWMIVNDYVSTLSCKVSGDS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IDMYRKLYATLEEKQENDALKIYDELVENIQFHS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSSNTKVYLEIVKKIRSIMEEDGLVAGDRLPSERELSSRLNVGRSSVREALRALELVGL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
IETRRGEGTFIRNFYDNGLVQLIAPFLLQDEKTIRDLLQTKRLLEKDMIRIVCNLPKETF
HHHCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKVLSKLHQVLEGNENSISMLHQTFFKTLIEQVDNYLLYRIWMIVNDYVSTLSCKVSGDS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IDMYRKLYATLEEKQENDALKIYDELVENIQFHS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]