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The map label for this gene is bctl

Identifier: 49478654

GI number: 49478654

Start: 4284582

End: 4285916

Strand: Reverse

Name: bctl

Synonym: BT9727_4221

Alternate gene names: 49478654

Gene position: 4285916-4284582 (Counterclockwise)

Preceding gene: 49478657

Following gene: 49478653

Centisome position: 81.83

GC content: 34.23

Gene sequence:

>1335_bases
GTGTTACACATGGGGAAAGATGTAAGTAACCATTCAAAATGGTTTGTTTTCACATTATGTTTTATCGTTTTATTAGGACC
GGTGAATGCGGTTTTATTTAATGTTGCGTTAGAGGATATGGCTCATGATTTATCCATTAGTCAATCGAAAGTAAGTTGGG
TTGTAGTAGGTTACTCCTTAGTTGTCGGTATTGGTTCGATGATATATGGGAAACTGGCTGATCGTTATAGTGTGAAAAAA
CTATTAATTATTTCAATCATCATATTTGTAGCGGGTTCTATTATTGGATTTGTAAATCAATCATATGCGATTGTCATTTT
TGCAAGATTAGTGCAGGCGAGTGGGGGCGCGGCGTTTATTGCGCTTAGTATGATTGCGGTAGCAAAATTAGTTGCTCCTG
CTAAGAAGCCTGGTGCTTTAGCGATGATTAGTTCTTCTATTGCGTTAGCGGTTGGTATTGGTCCTTTAGTTGGTGGGGCT
ATTACAAATACACTAGGGTGGCCATATTTATTTTTATTTATGATTATCTCAGTATTGGGGATTTTCTTGCTTATAAAATT
TATGCCAGGAGAAGCGCATCATACGGATGAAGTGTTTTATTTTGATTATATTGGAGCGGCGTTACTATTTGTATTTATTA
CGACTGTTTTGGTAGGTGTTAATATGAATAGTTGGCTATTTGTGTTATCGATAATTTCCTTATTTTTATTCACGGTTCGT
ATGAAGAAAGCGGAGCACCCATTTATCGATATTGAGTTATTTTCAAACAAAGCATTTCTTCGTTTAATAACAGTCGGATT
TATAATTAATGTGGCGTTATGTGCTAATTTATTATTATTGCCATTACTGTTAGGAAGAGTACACGGATTGTCGCCGTTTA
TTATCGGAGTTGTATTATTTGTTGCATCACTTTTCGGTATTGTGTCTAGTTTTATTACTGGAAAGATTATCCCTTCGTTT
GGAAATGTGAATATGATTTATGTAGCGTCTGTCATTATGATTGTTGGCTTTTTAATTTTAGGGTTTATTCCGAATGGAAG
TATAGTCGTTATTGTATTGGCGATTATTTTAACGTGTATGAGTTATTCTGCCATTCAAGTATCATTGAACACATTTATAC
CGAAAACATTACATGTAGCTAAAGTTGGAGTCGGTCTTGGTTTATATAATTTAATTAACTTTTTCGGTATGGCATTTGGA
CCAGCTGTAGCGAGCAAAATTATGGAATCCACAAATAGTTATCGTTTTAATTTTATTTTAATCGTCATGTTAATTTCTGC
TCATTTCTTCTTATTAATAGGAATGTCTTCTTTCCGAAAAAAGATGGAGCATTAA

Upstream 100 bases:

>100_bases
TTTGTCGCGCGATAAATGTATACATTTGATGAAGTTGTTTAGCAGCGATAGAAGCGCTAAACAACTTTATTTTCATTTAC
AGTTACTACAATCAGGAGAG

Downstream 100 bases:

>100_bases
ATATGTACTTTATATAGAAGAAACCGACTTTCCTATAGAAGGTCGGTTATTTTTCTGTCTTGCAAATTATGAAAAAAAGG
AGCAAAATAAAAAAGGTCCT

Product: tetracycline resistence protein, metal-tetracycline/H+ antiporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLHMGKDVSNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKK
LLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGA
ITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVR
MKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGVVLFVASLFGIVSSFITGKIIPSF
GNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTCMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFG
PAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH

Sequences:

>Translated_444_residues
MLHMGKDVSNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKK
LLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGA
ITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVR
MKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGVVLFVASLFGIVSSFITGKIIPSF
GNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTCMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFG
PAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH
>Mature_444_residues
MLHMGKDVSNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKK
LLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGA
ITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVR
MKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGVVLFVASLFGIVSSFITGKIIPSF
GNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTCMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFG
PAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=440, Percent_Identity=22.7272727272727, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788710, Length=280, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789042, Length=308, Percent_Identity=25, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081983, Length=392, Percent_Identity=23.469387755102, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=123, Percent_Identity=34.9593495934959, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=171, Percent_Identity=26.9005847953216, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48175; Mature: 48175

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2109312 [H]