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The map label for this gene is phoB [H]

Identifier: 49478615

GI number: 49478615

Start: 4145002

End: 4146387

Strand: Reverse

Name: phoB [H]

Synonym: BT9727_4082

Alternate gene names: 49478615

Gene position: 4146387-4145002 (Counterclockwise)

Preceding gene: 49478617

Following gene: 49480657

Centisome position: 79.16

GC content: 36.94

Gene sequence:

>1386_bases
GTGAAAAAGTTTGTGAAAAAAGCATGGCCATTTGCAGTTGTGGCATCATTAGCAGTTACTTCGGTAGCAACATGGAATTT
TACACGTTCTAGTGAAGTGAGCGCAGATGAGAACGGAAGCAATGCAAAGATTAAAAATGTTATTGTGTTAATTGGAGATG
GTATGGGCCCTTCATATATGACAGCTCACCGTTATATGAAAGATAATCCAAAAACATTCGAGATGGAATCTACAGAGTTT
GATAAACATCTTGTAGGAACACAAAAAACATATCCGGAAGATGAACATGAAAATATCACAGACTCAGCATCAGCAGCAAC
AGCTATGTCAGCAGGAATTAAAACATATAATGCGGCAATTGCAGTTGATAATGATAAAGCAGAAGTGAAAACTGTTCTTG
AGCAAGCGAAAGAGAAAGGGAAATCAACAGGATTAGTTGCTACTTCTGAAATTACACATGCAACACCAGCTGCTTTCGGT
GCGCATGACATTAGTCGTAAAAATATGGATGCAATTGCAAATGATTATTTTGATGAGAAAATTAAAGGGAAGCATAAAGT
AGATGTTATGCTTGGCGGCGGCGTAAAAAACTTTGTTAGAAAAGATCGCAATCTTACAGAAGAATTTAAGAAATCTGGTT
ATAGTTACGTAACAGATCGAGATCAATTGTTAAATGATAAAAATGATCAAATTCTTGGTTTATTTGCACCAGGCGGTTTA
GATAAAATGATTGACCGCAATGAGAAGACACCTTCATTAGAAGAAATGACAAATGCAGCAATTGAGCGTTTGAACAAAAA
TAAAAAAGGTTTCTTCTTAATGGTTGAAGGAAGTCAAATTGACTGGGCAGGACACGATAATGATATCGTTGGTGCAATGA
GTGAGATGGAAGACTTTGAAAGAGCATTTAAAGCTGCGATTGAGTTTGCGAAAAAAGATAAAAATACGTTAGTCATTGCA
ACGGCAGATCACGCTACTGGCGGGTTATCTTTAGGTGCGAATGGTGAGTACAACTTTAAAGTAGATCCAATTAAAGCTGC
AAAACGCACACCGGACTTCATGGCAAATGAGATTGCAAAAGGTGCAAATGTAGAAGAAACATTGAAAAAATATATTGATT
TACAATTAACACCTGAAGAAATTAAAGCGGTAAATGATATTGCTCCTTCAAAAGATGTAACAAAGATTGATAATGCGATT
GAAGATATTTTCAATAAGCGTTCGGTTACAGGATGGACAACAGGTGGACATACAGGAGAAGATGTAAACGTATATGCATT
TGGCCCTGGTAAATACTTGTTCTCTGGCGTTCAAGAGAATACAAATCTCGCTAAACGTGTCTTTGATATTGTTGGCGGTG
GTGATCCGAATAAAGGAAGACGCTAA

Upstream 100 bases:

>100_bases
ATAAGTTAACAAAAAATTTACATATAACTAATTTTCCCTTAACAGTCTTCTATTAAATTTGATAGTGGGAAATGAATAAC
CTAATTAGGGGGACAAGACA

Downstream 100 bases:

>100_bases
TTATAAATGAGAGTGAGGCGAAATGCCTCACTTTTCTTTTTAGGAGATGGGAAGATGAGAGCTTTTTTCAGGGGGATAGG
GCCCTTTCTAATAGGTGTAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFAPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFERAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Sequences:

>Translated_461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFAPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFERAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
>Mature_461_residues
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYMTAHRYMKDNPKTFEMESTEF
DKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAIAVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFG
AHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFAPGGL
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFERAFKAAIEFAKKDKNTLVIA
TADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAI
EDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=485, Percent_Identity=30.7216494845361, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI94721246, Length=485, Percent_Identity=29.8969072164948, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=30.5309734513274, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI116734717, Length=453, Percent_Identity=30.4635761589404, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI294660770, Length=448, Percent_Identity=29.9107142857143, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI294660772, Length=393, Percent_Identity=29.0076335877863, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI48994877, Length=340, Percent_Identity=38.2352941176471, Blast_Score=178, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320689, Length=449, Percent_Identity=34.9665924276169, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21355981, Length=453, Percent_Identity=30.6843267108168, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI18859923, Length=415, Percent_Identity=28.9156626506024, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355151, Length=451, Percent_Identity=28.8248337028825, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921912, Length=469, Percent_Identity=30.7036247334755, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI85725200, Length=444, Percent_Identity=29.7297297297297, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI85815833, Length=444, Percent_Identity=29.7297297297297, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24651554, Length=493, Percent_Identity=29.6146044624746, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358067, Length=451, Percent_Identity=29.2682926829268, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24657835, Length=453, Percent_Identity=28.6975717439294, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355149, Length=464, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24657827, Length=456, Percent_Identity=30.4824561403509, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585213, Length=466, Percent_Identity=28.1115879828326, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24657842, Length=455, Percent_Identity=27.9120879120879, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24651556, Length=451, Percent_Identity=29.0465631929047, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50538; Mature: 50538

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYM
CCHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDNPKTFEMESTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
AVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFGAHDISRKNMDAIANDYFDEK
EECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
IKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFAPGGL
HCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHH
RAFKAAIEFAKKDKNTLVIATADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAIEDIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKFVKKAWPFAVVASLAVTSVATWNFTRSSEVSADENGSNAKIKNVIVLIGDGMGPSYM
CCHHHHHHCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHH
TAHRYMKDNPKTFEMESTEFDKHLVGTQKTYPEDEHENITDSASAATAMSAGIKTYNAAI
HHHHHHCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEE
AVDNDKAEVKTVLEQAKEKGKSTGLVATSEITHATPAAFGAHDISRKNMDAIANDYFDEK
EECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
IKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKSGYSYVTDRDQLLNDKNDQILGLFAPGGL
HCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCEECCHHHHHCCCCCCEEEEECCCCH
DKMIDRNEKTPSLEEMTNAAIERLNKNKKGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFE
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHH
RAFKAAIEFAKKDKNTLVIATADHATGGLSLGANGEYNFKVDPIKAAKRTPDFMANEIAK
HHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHC
GANVEETLKKYIDLQLTPEEIKAVNDIAPSKDVTKIDNAIEDIFNKRSVTGWTTGGHTGE
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
DVNVYAFGPGKYLFSGVQENTNLAKRVFDIVGGGDPNKGRR
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]