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The map label for this gene is bioF

Identifier: 49478538

GI number: 49478538

Start: 3942872

End: 3944059

Strand: Reverse

Name: bioF

Synonym: BT9727_3859

Alternate gene names: 49478538

Gene position: 3944059-3942872 (Counterclockwise)

Preceding gene: 49478539

Following gene: 49478537

Centisome position: 75.3

GC content: 38.13

Gene sequence:

>1188_bases
ATGAATCAACCGTGGCGCACACATCTTCAAACTAAGTTAAAACAATTACATGAGCAAGGGCAGTATCGCAATTTGCACGT
AACAGAACAAGCAGAAGAGACATGGCTTATTCGAGATGAGAAAAGGATGCTAAATTTAGCGTCAAATAATTATTTAGGAT
TAGCTGGAGATGAAAGGTTAAAAGAAGCTGCTATCGTATGCACAAGAAAATATGGAACTGGGGCGACAGCATCAAGGCTT
GTGGTAGGAAACTATTCACTGTATGAAGAGGTTGAGAGAAGTATATGCAATTGGAAAGGCACTGAAAAAGCCTTAGTTGT
AAATAGTGGATTTACCGCAAATGTTGGAGCGATTTCTTCATTAGTCTGTCGTCACGATATTGTTTTTAGCGATAAATTAA
ATCATGCGAGCATTGTTGATGGAATTATATTAAGCGGGGCAGAGCATAAAAGGTATCGACATAATGATTTAAATCATCTG
GAGGCGTTGTTGAAAACAGCATCTCCTGAAAAGCGGAAATTAATCGTAACAGATACTGTTTTTAGTATGGATGGTGATAC
TGCGCATTTGAGGGAGTTAGTGCAGCTGAAAGAGAAATACGGGGCAATCATTATAGTTGATGAAGCACATGCGAGTGGAA
TATATGGAATTGGTGGAGCAGGGTTATCTCATATAGAAAAAGATCTTGCTCAAAAAATAGATATACATATGGGGACGTTT
AGCAAGGCTTTAGGGTGTTATGGTGCGTATTTGACAGGTGATGCAATTTATATAGAGTATTTGCAAAATATGATGAGAAG
CTTTATTTTTACTACAGCTTTACCGCCAAGTACGTTAGGAGCAGTGCAAAAAGCAATTGAAATTGTACAAGAAGATCATA
AGAGAAGAGAGAACCTTATAGCAAATGGAGAATACTTCAGGAGTAAATTACGAGAAGCTGGATTTAATATAGGGAATAGT
TCAACTCATATTGTACCGATTGTAGTTGGTTCAAATGAAAATACTTTACGGTTTAGCAAAAGGTTACAAGAGGCAGGTAT
TGCAGCTATTGCAATTCGTCCGCCCACTGTACCGGTCCATAGTTCTCGAATTCGCTTTGCAGTTACATCTCAACATACAA
TAGCTGATTTAAAATGGGCTATTGACCGAATTACCCACATTGCGAAAGAAGAGGAGCTTTTCGTATGA

Upstream 100 bases:

>100_bases
GGCTTATTACCATTCTTTGAAGGTGAGTTTACAAAAGAAGAAGTATTGGAATCTGCTAAAGAATATATAATGATTTCAAA
ATTAGAGGAGTTCATCCGAA

Downstream 100 bases:

>100_bases
AAGAGCTGAAAATCATTTTTATCCCTGGATGGGGAATGGAAGAAAATATTTGGGATTTAGTACTTCCGCATTTTAAAGGA
TATTCTGTTCAATGCATTGA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase 2; 7-keto-8-amino-pelargonic acid synthase 2; 7-KAP synthase 2; KAPA synthase 2; 8-amino-7-ketopelargonate synthase 2; Alpha-oxoamine synthase 2; L-alanine--pimeloyl-CoA ligase 2 [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNQPWRTHLQTKLKQLHEQGQYRNLHVTEQAEETWLIRDEKRMLNLASNNYLGLAGDERLKEAAIVCTRKYGTGATASRL
VVGNYSLYEEVERSICNWKGTEKALVVNSGFTANVGAISSLVCRHDIVFSDKLNHASIVDGIILSGAEHKRYRHNDLNHL
EALLKTASPEKRKLIVTDTVFSMDGDTAHLRELVQLKEKYGAIIIVDEAHASGIYGIGGAGLSHIEKDLAQKIDIHMGTF
SKALGCYGAYLTGDAIYIEYLQNMMRSFIFTTALPPSTLGAVQKAIEIVQEDHKRRENLIANGEYFRSKLREAGFNIGNS
STHIVPIVVGSNENTLRFSKRLQEAGIAAIAIRPPTVPVHSSRIRFAVTSQHTIADLKWAIDRITHIAKEEELFV

Sequences:

>Translated_395_residues
MNQPWRTHLQTKLKQLHEQGQYRNLHVTEQAEETWLIRDEKRMLNLASNNYLGLAGDERLKEAAIVCTRKYGTGATASRL
VVGNYSLYEEVERSICNWKGTEKALVVNSGFTANVGAISSLVCRHDIVFSDKLNHASIVDGIILSGAEHKRYRHNDLNHL
EALLKTASPEKRKLIVTDTVFSMDGDTAHLRELVQLKEKYGAIIIVDEAHASGIYGIGGAGLSHIEKDLAQKIDIHMGTF
SKALGCYGAYLTGDAIYIEYLQNMMRSFIFTTALPPSTLGAVQKAIEIVQEDHKRRENLIANGEYFRSKLREAGFNIGNS
STHIVPIVVGSNENTLRFSKRLQEAGIAAIAIRPPTVPVHSSRIRFAVTSQHTIADLKWAIDRITHIAKEEELFV
>Mature_395_residues
MNQPWRTHLQTKLKQLHEQGQYRNLHVTEQAEETWLIRDEKRMLNLASNNYLGLAGDERLKEAAIVCTRKYGTGATASRL
VVGNYSLYEEVERSICNWKGTEKALVVNSGFTANVGAISSLVCRHDIVFSDKLNHASIVDGIILSGAEHKRYRHNDLNHL
EALLKTASPEKRKLIVTDTVFSMDGDTAHLRELVQLKEKYGAIIIVDEAHASGIYGIGGAGLSHIEKDLAQKIDIHMGTF
SKALGCYGAYLTGDAIYIEYLQNMMRSFIFTTALPPSTLGAVQKAIEIVQEDHKRRENLIANGEYFRSKLREAGFNIGNS
STHIVPIVVGSNENTLRFSKRLQEAGIAAIAIRPPTVPVHSSRIRFAVTSQHTIADLKWAIDRITHIAKEEELFV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=33.6206896551724, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI284448556, Length=348, Percent_Identity=33.6206896551724, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=32.3287671232877, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI119220554, Length=351, Percent_Identity=31.3390313390313, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI83977442, Length=336, Percent_Identity=33.6309523809524, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI83977440, Length=336, Percent_Identity=33.6309523809524, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI83977444, Length=336, Percent_Identity=33.6309523809524, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4502025, Length=346, Percent_Identity=31.7919075144509, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI40316939, Length=346, Percent_Identity=31.7919075144509, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI5454084, Length=262, Percent_Identity=30.5343511450382, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790046, Length=394, Percent_Identity=33.248730964467, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1786993, Length=360, Percent_Identity=32.5, Blast_Score=221, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=392, Percent_Identity=32.9081632653061, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI32566772, Length=372, Percent_Identity=30.9139784946237, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560912, Length=368, Percent_Identity=27.1739130434783, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17560914, Length=368, Percent_Identity=27.1739130434783, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71982617, Length=285, Percent_Identity=24.9122807017544, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71982625, Length=285, Percent_Identity=24.9122807017544, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320267, Length=367, Percent_Identity=28.3378746594005, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320438, Length=387, Percent_Identity=29.9741602067183, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323954, Length=267, Percent_Identity=29.9625468164794, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24662918, Length=388, Percent_Identity=33.7628865979381, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI17137420, Length=346, Percent_Identity=33.8150289017341, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17136286, Length=369, Percent_Identity=31.1653116531165, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653280, Length=280, Percent_Identity=27.1428571428571, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653276, Length=280, Percent_Identity=27.1428571428571, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24653278, Length=280, Percent_Identity=27.1428571428571, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 44101; Mature: 44101

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPWRTHLQTKLKQLHEQGQYRNLHVTEQAEETWLIRDEKRMLNLASNNYLGLAGDERL
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECHHHHHHHCCCCEEECCCHHHH
KEAAIVCTRKYGTGATASRLVVGNYSLYEEVERSICNWKGTEKALVVNSGFTANVGAISS
HHHHHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHH
LVCRHDIVFSDKLNHASIVDGIILSGAEHKRYRHNDLNHLEALLKTASPEKRKLIVTDTV
HHHHHCEEECCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEE
FSMDGDTAHLRELVQLKEKYGAIIIVDEAHASGIYGIGGAGLSHIEKDLAQKIDIHMGTF
EECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SKALGCYGAYLTGDAIYIEYLQNMMRSFIFTTALPPSTLGAVQKAIEIVQEDHKRRENLI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ANGEYFRSKLREAGFNIGNSSTHIVPIVVGSNENTLRFSKRLQEAGIAAIAIRPPTVPVH
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCC
SSRIRFAVTSQHTIADLKWAIDRITHIAKEEELFV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQPWRTHLQTKLKQLHEQGQYRNLHVTEQAEETWLIRDEKRMLNLASNNYLGLAGDERL
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECHHHHHHHCCCCEEECCCHHHH
KEAAIVCTRKYGTGATASRLVVGNYSLYEEVERSICNWKGTEKALVVNSGFTANVGAISS
HHHHHEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHH
LVCRHDIVFSDKLNHASIVDGIILSGAEHKRYRHNDLNHLEALLKTASPEKRKLIVTDTV
HHHHHCEEECCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEE
FSMDGDTAHLRELVQLKEKYGAIIIVDEAHASGIYGIGGAGLSHIEKDLAQKIDIHMGTF
EECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SKALGCYGAYLTGDAIYIEYLQNMMRSFIFTTALPPSTLGAVQKAIEIVQEDHKRRENLI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ANGEYFRSKLREAGFNIGNSSTHIVPIVVGSNENTLRFSKRLQEAGIAAIAIRPPTVPVH
CCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCC
SSRIRFAVTSQHTIADLKWAIDRITHIAKEEELFV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]