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The map label for this gene is cycB [H]

Identifier: 49478493

GI number: 49478493

Start: 3850183

End: 3851442

Strand: Reverse

Name: cycB [H]

Synonym: BT9727_3754

Alternate gene names: 49478493

Gene position: 3851442-3850183 (Counterclockwise)

Preceding gene: 49479365

Following gene: 49481603

Centisome position: 73.53

GC content: 37.14

Gene sequence:

>1260_bases
ATGAAGAAAGCATTATCATTATTAACTGTTTCAGCATTATCAATTGGTATGTTATCTGCATGCGGACCGAAAGATTCAGG
GAAAAAAGAAGAAAGTAAAGCAAAGAAAGATTACGATCTACTCGTTTGGGAAGATGATAAAAAAGGTGTTGGTCTAGAAC
CAGCAGTGAAAAGCTTTGAGGAAAAATATAAAGTAAAAGTAAAAGTTGTTGAAATGCAAATGACAGATCAAGTGAAGAAG
TTACGTCTTGATGGACCAGCGGGGACGGGACCAGATGTTGTAACATTACCACATGATCAAATCGGAAACGCAGTAACAGA
AGGTTTACTTTCTGAAGTGAAAGCTGATGATGCTGTAAAGAGTAAATTTACTGATTCATCAATAGAAGCACAAACGTATA
ACGGAAAATTATATGGTTTACCAAAAGCAATTGAGACACCAGTCTTTATTTACAATAAAAAGCTAATGCCAAAAGCGCCA
GAAACGATGGACGAGCTGTTTAACTTCTCAAAAGAATTTACGAAAGATGGCAAGTACGGATTTTTAGCATTAGGAGATAA
CTTCTACTTTGCTAATGCATTTATGGCAGGTATGGGTGGCTATGTGTTTGGTGAAAAAGATGGAAAACCAAATGCAAGTG
ATGTTGGATTAAATAATAGCGGAGCAGTACAAGGTGCTGAGTATCTTCAAAAATGGTACAAAGAAAAATTATTCCCGAAA
GGTATTATCGGTGAATCTGGTGGACCTGCTGCTGATGGACTATTTAACGAAGGAAAAGCAGCATCTATCATGAACGGCCC
TTGGGCGTTCCAAGCAATGGAAAAGAACGGAATTGATTATGGCGTTGCTCCAATGCCGAAATTACCAAATGGTCAACCAT
TGAAAACGTTTGTCGGGGTTAAAGGATGGCATGTAACAAGTTTCTCTAAACAAAATGATTTAGCTACAAAGTTCACTGAA
TGGGTAACGAATGAAGAAAACGCAAAAATTCGATTTGAGAAGACGAAAGAAATTCCTCCAGTAAAAGCAGTAATGGAAGA
TCCAATTATTAAAGATAACGAAGCTGCAAAAGCAGTTGCAACACAATCTGAAAATGGAATTCCAATGCCAAACATTCCAG
AAATGCAAGAAGTATGGAAACCAGCTGGAGATGCGCTTCAATTAGTTGTTACGGATAAAGAGGCACCAAAAGCAGCACTT
GATAATGCAGTGAAGCAAATTAAAGGTAATATTGAGGCAAATCATAGTAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GTTTTCATAGATTGATATATTGTTTGCGCTTCTATTAAATTATTTAATGCAAACGTTTTCAATTAGTAAGGTACATATAC
AATTTAGGGAGGTTTTTGGG

Downstream 100 bases:

>100_bases
GGTGAAATTCGTGCCTCTAATATGAGGGGCACGAATTCTCTCCCAAGAAAAAAGGGGGAAGGAACATGCAAACATCTATG
GAACCAGCAAACGGCTTAAA

Product: maltose-maltodextrin ABC-related solute binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKKALSLLTVSALSIGMLSACGPKDSGKKEESKAKKDYDLLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKK
LRLDGPAGTGPDVVTLPHDQIGNAVTEGLLSEVKADDAVKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPKAP
ETMDELFNFSKEFTKDGKYGFLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDVGLNNSGAVQGAEYLQKWYKEKLFPK
GIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNGIDYGVAPMPKLPNGQPLKTFVGVKGWHVTSFSKQNDLATKFTE
WVTNEENAKIRFEKTKEIPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAPKAAL
DNAVKQIKGNIEANHSKKK

Sequences:

>Translated_419_residues
MKKALSLLTVSALSIGMLSACGPKDSGKKEESKAKKDYDLLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKK
LRLDGPAGTGPDVVTLPHDQIGNAVTEGLLSEVKADDAVKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPKAP
ETMDELFNFSKEFTKDGKYGFLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDVGLNNSGAVQGAEYLQKWYKEKLFPK
GIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNGIDYGVAPMPKLPNGQPLKTFVGVKGWHVTSFSKQNDLATKFTE
WVTNEENAKIRFEKTKEIPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAPKAAL
DNAVKQIKGNIEANHSKKK
>Mature_419_residues
MKKALSLLTVSALSIGMLSACGPKDSGKKEESKAKKDYDLLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKK
LRLDGPAGTGPDVVTLPHDQIGNAVTEGLLSEVKADDAVKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPKAP
ETMDELFNFSKEFTKDGKYGFLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDVGLNNSGAVQGAEYLQKWYKEKLFPK
GIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNGIDYGVAPMPKLPNGQPLKTFVGVKGWHVTSFSKQNDLATKFTE
WVTNEENAKIRFEKTKEIPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAPKAAL
DNAVKQIKGNIEANHSKKK

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=418, Percent_Identity=31.5789473684211, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45658; Mature: 45658

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALSLLTVSALSIGMLSACGPKDSGKKEESKAKKDYDLLVWEDDKKGVGLEPAVKSFE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EKYKVKVKVVEMQMTDQVKKLRLDGPAGTGPDVVTLPHDQIGNAVTEGLLSEVKADDAVK
HHHEEEEEEEEEHHHHHHHHEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
SKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPKAPETMDELFNFSKEFTKDGKYG
HHCCCCCCEEEEECCEEEECCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEE
FLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDVGLNNSGAVQGAEYLQKWYKEKLFPK
EEEECCCEEEHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNGIDYGVAPMPKLPNGQPLKTFVGV
CCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHCC
KGWHVTSFSKQNDLATKFTEWVTNEENAKIRFEKTKEIPPVKAVMEDPIIKDNEAAKAVA
CCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHH
TQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAPKAALDNAVKQIKGNIEANHSKKK
CCCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKALSLLTVSALSIGMLSACGPKDSGKKEESKAKKDYDLLVWEDDKKGVGLEPAVKSFE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EKYKVKVKVVEMQMTDQVKKLRLDGPAGTGPDVVTLPHDQIGNAVTEGLLSEVKADDAVK
HHHEEEEEEEEEHHHHHHHHEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
SKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPKAPETMDELFNFSKEFTKDGKYG
HHCCCCCCEEEEECCEEEECCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEE
FLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDVGLNNSGAVQGAEYLQKWYKEKLFPK
EEEECCCEEEHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNGIDYGVAPMPKLPNGQPLKTFVGV
CCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHCC
KGWHVTSFSKQNDLATKFTEWVTNEENAKIRFEKTKEIPPVKAVMEDPIIKDNEAAKAVA
CCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHH
TQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAPKAALDNAVKQIKGNIEANHSKKK
CCCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]