Click here to switch to the map view.

The map label for this gene is pbpB [H]

Identifier: 49478447

GI number: 49478447

Start: 3758849

End: 3760999

Strand: Reverse

Name: pbpB [H]

Synonym: BT9727_3658

Alternate gene names: 49478447

Gene position: 3760999-3758849 (Counterclockwise)

Preceding gene: 49479387

Following gene: 49478890

Centisome position: 71.81

GC content: 38.21

Gene sequence:

>2151_bases
ATGAAAAGGAATATGACTAAATTTCATACCAATAAGGGAGCAGGGTATTTTATGATTTTATTCCTCCTGCTCTTTTTGCT
GTTATTAGCCCGATTTTTTTACATACAAGCGACAGGAACAGTGCATAATCAGGATTTAGATACACTTGCTAAGCAAAAAC
ATAGTAAAACAGGAGTTCTGGAAGCGAATCGTGGGACGATTTATGATCAAAATTCCCATGTGTTAGCGCAAGATGCAAAC
TCTTATAAACTTGTAGCGGAATTAAAAGGTGCGAAGCCGGTTGAGAATAAAGAGGATACTGCTAAGAAAATTGCGGGAGT
ACTCGGAACAGAAGAAGAGAAGATTTTAGCTACTTTAAATAAAGAGGGGAGAAGTCAAGTAGAGTTTGGGAAATTAGGTA
AAGGCTTGACGAAAGAACAGAAAGAACAAATAGAAGCATTGAAATTACCAGGTATATCTTTTATTACTGAAAATGCAAGA
GTGTATCCGAACGGTGATTTTGCATCTTATGTTGTAGGTCATGCGAGACCTGATGAAAAGGGAATCGCTGTAGGTCAATT
TGGTTTAGAAAAGAGTTTGAATAAGTATTTAGGTGCTTCCAATGGAGAGGTAGCTTATACAGGCGATCGTAGAGGCGTAT
CTCTTGACGGTGGAAAAGTGAATGTGAAGGCACCTAAAAATGGAGATAATGTATATTTAACACTTGACCAGCGTATTCAA
AGTTATTTAGAAGATGCAATGAAAGAAGCGAGTAAACATTATGAGCCAGAAAGCTTAATAGGAATTGTTGCGGATCCAAA
AACAGGGAAAATATTAGCGATGTCTAGTAAGCCTAGTTATGATCCGAACCAAGAGAATAATAATTATTTTTATAATGACG
CAATTGCAAATGCATTTGAACCTGGATCAACAATGAAAATTTTCACACTAGCGGCAGCTATTAATGAAGGTGTGTATAAG
GGACAAGATTATTATCAGTCTGGTACATATCCAGTCGGGAATCGAAAAATAAAGGATCATAATGGCGGCGCTGGATGGGG
ATCTATTACTTTTGATGAAGGGGTAGAGAGATCTTCCAACGTAGCGTTTGCAATTTTAGGGGATCAAAAACTTGGTCCAG
ACCGTTTCCGAAAGTATATTCACGCTTTCGGATTAGATGAAAAAACGAATATTGATTTACCTGGTGAAGGTTCAAATACC
ATTGTATTCGATCAGCAAATACAGCAAGTAACAACAGCTTTTGGACAAGGTTCTACTGTAACACCAATTCAGCTTGTACA
AGCTGCAACTGCTATAGCAAATGATGGGAAAATGATGAAACCTTATACAATTGATAAAATTGTAGATCCGATAACAGAGG
AAGTAAAATTAGAACATAAACCAGAAGAAGTGGGAAAACCTGTTACAAAAGAGACAGCAGCGCAAGTAAGACAGTTATTA
GAGCGTGTTGTTACATCACCGAAAGGAACAGGGACTGCTTATAAAGTCGATGGATATTCAGTTGGTGGTAAAACAGGAAC
AGCACAAATTCCGGATGGAAAAGGTGGCTATATGACGGGAAGAGAGAATTATATATTCTCGTTCCTAGGTATGGCACCTA
TGGACGATCCGCAACTTGTTGTGTATGTAGCTGTTAAAAAGCCGAAATTGAAAGATGATGAGAGCGGTGCACAACCTTTA
GCTGATATTTTTAAATATGTAACGAAGAATAGTTTAGAGTATTTAAAGATTAAGCCTAATGAAGTGAAAGATCCGAAGAA
ATATGTGAAAGAGCAACAAACTGCTGTTCCAGATGTAACCGAAAAAACGATGGAAGAAGCGAAAAAAGCCATTGATAAAG
CAAAACTTCGTCCAATCGTATTAGGTGAAGGGAAAGTACAGCAACAAGTACCGAAAGCAACTGAACAAACATTGAAGGGT
GACCGAGTCTTCTTAGTGGGAGATAAACCTACAATGCCGAATATACAAGGCTGGGCACTGCGTGATGTTATGAATTTAGC
AAAAACATTAAAGCTTAACCTAAAACCTACTGGTACCGGATACGTTACTGAACAGAGTGTGCCAGAAGGAACATTGTTAC
AACCTGGTACAGAGTTAGGTGTAACACTTGTACCACCACTGGAACCACAACAAGAAGCAGAAAAACCGTAA

Upstream 100 bases:

>100_bases
TCGTTATGAACGAATCGCTGAAGTTGCAAAAAAACACGGGCTAGAAATTAATGCAAATAATGTGAAAGGCCTCAAATAAG
GCAATAGGTGTGAAGGGAAA

Downstream 100 bases:

>100_bases
TGGTAAAAGAGAAGATGTTCTAATTAAATAAGTACAAGCATATATTGGAACAAGCTAGGCGTAAGGGAGGCTTGTTCCAA
TATGCGTGTATCAAATGTAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGTVHNQDLDTLAKQKHSKTGVLEANRGTIYDQNSHVLAQDAN
SYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDEKGIAVGQFGLEKSLNKYLGASNGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILAMSSKPSYDPNQENNNYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPDRFRKYIHAFGLDEKTNIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Sequences:

>Translated_716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGTVHNQDLDTLAKQKHSKTGVLEANRGTIYDQNSHVLAQDAN
SYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDEKGIAVGQFGLEKSLNKYLGASNGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILAMSSKPSYDPNQENNNYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPDRFRKYIHAFGLDEKTNIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
>Mature_716_residues
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGTVHNQDLDTLAKQKHSKTGVLEANRGTIYDQNSHVLAQDAN
SYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENAR
VYPNGDFASYVVGHARPDEKGIAVGQFGLEKSLNKYLGASNGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
SYLEDAMKEASKHYEPESLIGIVADPKTGKILAMSSKPSYDPNQENNNYFYNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPDRFRKYIHAFGLDEKTNIDLPGEGSNT
IVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAVKKPKLKDDESGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=602, Percent_Identity=27.4086378737542, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1786854, Length=548, Percent_Identity=23.7226277372263, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78460; Mature: 78460

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGTVHNQDLDTLAKQKHSKTGVL
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCCCEE
EANRGTIYDQNSHVLAQDANSYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHC
KEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDEK
CCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHEECCCCCCC
GIAVGQFGLEKSLNKYLGASNGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
CEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHH
SYLEDAMKEASKHYEPESLIGIVADPKTGKILAMSSKPSYDPNQENNNYFYNDAIANAFE
HHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEEEHHHHHHHCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPDRFRKYIHAFGLDEKTNIDLPGEGSNTIVFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYVAVKKPKLKDDESGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHH
EKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
HHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEEECCCCCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC
>Mature Secondary Structure
MKRNMTKFHTNKGAGYFMILFLLLFLLLLARFFYIQATGTVHNQDLDTLAKQKHSKTGVL
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCCCEE
EANRGTIYDQNSHVLAQDANSYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHC
KEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDEK
CCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHHEECCCCCCC
GIAVGQFGLEKSLNKYLGASNGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQ
CEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHH
SYLEDAMKEASKHYEPESLIGIVADPKTGKILAMSSKPSYDPNQENNNYFYNDAIANAFE
HHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEEEHHHHHHHCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPDRFRKYIHAFGLDEKTNIDLPGEGSNTIVFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKVDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYVAVKKPKLKDDESGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHH
EKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
HHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEEECCCCCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]