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The map label for this gene is cbaH

Identifier: 49478365

GI number: 49478365

Start: 3595591

End: 3596577

Strand: Reverse

Name: cbaH

Synonym: BT9727_3508

Alternate gene names: 49478365

Gene position: 3596577-3595591 (Counterclockwise)

Preceding gene: 49479185

Following gene: 49480843

Centisome position: 68.67

GC content: 36.78

Gene sequence:

>987_bases
ATGTGTACTAGTTTGACATTAGAGACAAAAAACGGTCAGCATCTTTTTGCAAGAACGATGGACTTCACATTAGATATGAA
TCAAGAAGTAATAATCATTCCTCGACATTACCAGTGGAATAATATAACGGGTGAAATCATTAATACGAAACATGCTACGG
TTGGAATGGGAATTAATCATCAAGGAAGGATCATTATGGCGGACGGAGTAAATGAGGCAGGTATGACATGCGCAACACTC
TATTTTCCAGGATTCGCTACTTATAGTCAAAGCATAGATGACAATAAAATGAATTTGGCTCCATTTGATTTTGTAGCTTG
GAGTCTTACACAATTCAATTCTGTCAAAGAGTTAAAGAAATCTGTAGATAGCATTACCTTTTTGGATATACCATTACCGG
ATTTAGGATTTACGCCACCACTACATTGGATTTTAGCGGATAAATGGGGAGATTGCATTGTACTGGAGCCGACAAGTGAA
GGATTAAAATTGTATGATAACCCACTAGGAGTGATGACGAATAGTCCGGAGTTTAATTGGCATTTGCAAAATTTAAGACA
ATATATAGGCCTTAAATCGCAGCCATTCGCGCCAACAGAGTGGAGTAATTTACCATTAAGTGCTTTTGGCCAAGGCTCAG
GTTCAATGGGACTTCCAGGGGATTTCACCCCACCATCAAGGTTTGTGCGGGCAGCATATGGCAAACAAAATATTCAAGGT
ATAGATAACGAAGAAGAGGGAGTATCAGCCCTTTTTCATATCTTATCAAATTGCGAGATTCCTAAAGGCGGAGTAATAAC
AGAAGAAGGTACATTAGATAATACTATTTATACAAGCGTAATGTGTATGGAGTCCGGAACATACTATTATCATACTTACG
ATTGTAGACAAATTATAGCTATTCATTTATTTCATGAAAATTTAGATACAGATGAGATTAAAGCCTATCCGTTCCAACGG
AAACAAAAAATATTTTATGAAAATTAA

Upstream 100 bases:

>100_bases
TATGTGCTTTCTCATTTTTTGTAAGGAAAGAGGTTTTTAATTTACAGATTTATCAGAATTATGTAAAGTGTAGATAAAAT
CATAAAATGGGGTGTTCTTT

Downstream 100 bases:

>100_bases
GTAGCTTTGAAACAACGTTTGGTAAATTTTAAAATATATATAATAGAGCAATAATGCCTATAAAAAGTAGGGATTGGAAG
GGATATAAATTCAATCCAAT

Product: choloylglycine hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCTSLTLETKNGQHLFARTMDFTLDMNQEVIIIPRHYQWNNITGEIINTKHATVGMGINHQGRIIMADGVNEAGMTCATL
YFPGFATYSQSIDDNKMNLAPFDFVAWSLTQFNSVKELKKSVDSITFLDIPLPDLGFTPPLHWILADKWGDCIVLEPTSE
GLKLYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFAPTEWSNLPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IDNEEEGVSALFHILSNCEIPKGGVITEEGTLDNTIYTSVMCMESGTYYYHTYDCRQIIAIHLFHENLDTDEIKAYPFQR
KQKIFYEN

Sequences:

>Translated_328_residues
MCTSLTLETKNGQHLFARTMDFTLDMNQEVIIIPRHYQWNNITGEIINTKHATVGMGINHQGRIIMADGVNEAGMTCATL
YFPGFATYSQSIDDNKMNLAPFDFVAWSLTQFNSVKELKKSVDSITFLDIPLPDLGFTPPLHWILADKWGDCIVLEPTSE
GLKLYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFAPTEWSNLPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IDNEEEGVSALFHILSNCEIPKGGVITEEGTLDNTIYTSVMCMESGTYYYHTYDCRQIIAIHLFHENLDTDEIKAYPFQR
KQKIFYEN
>Mature_328_residues
MCTSLTLETKNGQHLFARTMDFTLDMNQEVIIIPRHYQWNNITGEIINTKHATVGMGINHQGRIIMADGVNEAGMTCATL
YFPGFATYSQSIDDNKMNLAPFDFVAWSLTQFNSVKELKKSVDSITFLDIPLPDLGFTPPLHWILADKWGDCIVLEPTSE
GLKLYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFAPTEWSNLPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IDNEEEGVSALFHILSNCEIPKGGVITEEGTLDNTIYTSVMCMESGTYYYHTYDCRQIIAIHLFHENLDTDEIKAYPFQR
KQKIFYEN

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 37020; Mature: 37020

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSLTLETKNGQHLFARTMDFTLDMNQEVIIIPRHYQWNNITGEIINTKHATVGMGINH
CCCEEEEECCCCCEEEEEEEEEEEECCCCEEEEECEEECCCCCEEEEECCCEEEECCCCC
QGRIIMADGVNEAGMTCATLYFPGFATYSQSIDDNKMNLAPFDFVAWSLTQFNSVKELKK
CCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SVDSITFLDIPLPDLGFTPPLHWILADKWGDCIVLEPTSEGLKLYDNPLGVMTNSPEFNW
HHCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEECCCCCEEECCCCCCE
HLQNLRQYIGLKSQPFAPTEWSNLPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
EHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
IDNEEEGVSALFHILSNCEIPKGGVITEEGTLDNTIYTSVMCMESGTYYYHTYDCRQIIA
CCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEECCEEEEEEECHHEEEE
IHLFHENLDTDEIKAYPFQRKQKIFYEN
EEEEECCCCCCCEEECCCCHHCCCCCCC
>Mature Secondary Structure
MCTSLTLETKNGQHLFARTMDFTLDMNQEVIIIPRHYQWNNITGEIINTKHATVGMGINH
CCCEEEEECCCCCEEEEEEEEEEEECCCCEEEEECEEECCCCCEEEEECCCEEEECCCCC
QGRIIMADGVNEAGMTCATLYFPGFATYSQSIDDNKMNLAPFDFVAWSLTQFNSVKELKK
CCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SVDSITFLDIPLPDLGFTPPLHWILADKWGDCIVLEPTSEGLKLYDNPLGVMTNSPEFNW
HHCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEECCCCCEEECCCCCCE
HLQNLRQYIGLKSQPFAPTEWSNLPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
EHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
IDNEEEGVSALFHILSNCEIPKGGVITEEGTLDNTIYTSVMCMESGTYYYHTYDCRQIIA
CCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEECCEEEEEEECHHEEEE
IHLFHENLDTDEIKAYPFQRKQKIFYEN
EEEEECCCCCCCEEECCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]